MED14_DROME - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MED14_DROME
• UniProt AC: Q9W0P8
• Protein Name: Mediator of RNA polymerase II transcription subunit 14
• Gene Name: MED14
• Organism: Drosophila melanogaster (Fruit fly).
• Sequence Length: 1553
• Subcellular Localization: Nucleus .
• Protein Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for activated transcription of the MtnA, MtnB and MtnD genes..
• Protein Sequence: MAPTPLPLEQMPGVGGGGGGYLPAAQEGGPRINTMSMSVLIDFIIQRTYHELTVLAELLPRKTDMERKVEIYNYAARTRHLFTRLNALVKWGNSVSKVDKSSQIMSFLDKQNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPADAITPAEKRQTLLRLNQVIQHRLVTGKLLPQMREFRIRNGRVTFEVKHEFSVSLTVMGDNPTVPWRLLDIDVLVEDKETGDGKSLVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLNYERLDDNNITVEEYVPGVKLTVSYWRDLKSELGYRLTVQSDPSEIGRPLAVVHVPSLGAKESAEVADRAVRSEHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDFNLQGTPAILTVPVLSPCLRAEQIHITIDTHTGMFRCHVPKHLDCPIMEEMQDCLNGDRSKLPALLSELRFWITHRRCDKTLQHLPATATETLPFLVQPDQEILQPGRHKIYVKLHRHPNIVLVVQLKEKTTMPNEMEYTFHLGFVAYQKDELDVIDDSAMQLVSIVAQPHSDIPKCYTKLMRLIEFDTFVATHGPGTEVDAEVSPHKRKSNGDLLAPPAKQQKTIFPAYFIPELAHVVAMCDEKIPFMNLAQTLSKHNIPHSGLQVEANATSLVLKILALPQPGKTTFATQQQQQQGAPAVAGENKPSGTSGLPKIDSHVWDDLMRRVLSISIRSQTNKNSQVRIWVVEFVFYSTPLQSSHPKEQGSRRTVYLTYEQANYDFSKTVEDLLNDWSKIVYLYTLVYDFAEQLLNKRLSLCDMLVVKSYSYMNLLLGYGPKKEVSCNIYWSVQSHGFRLTFVGGMSAVNGHSMMRDQLAQHLNQQHSITQIAQILHETYNPLSSIAKLPVLPFLGIPRPQVPVLSFCVLAQSPCLIRLTYQAVYCLELRFRANRLVSIRDGASSRFERNVVEEFTPIQGLKAFLSKYVDESAVYRGRASHEDDNPLSPMGMEDNFGGPSSVAGVSAGGSSPFLGTGMRGPQSPRDSGLRFPAPHTPPSSSNPHTPASPHPSAGAGGGSGPQGHGNFNLTSPPAPHMPHPSPSGLMPSSPLNPQPSPHMVHSPGPNTLYMQSHQDSPFTAMSPANNNWPGSPSMPRPSPRPGQSPDHKSTGGGAGVAGGTDRGGSRGTLNRPWAGAVPTLLTHEALETLCRPSPYPNKDINVPDMSPLERFLGCVYMRRQLHRNIQSEETLTALNSTEPGVVLFKVDGLQCQVVLNQMHMQTLHLKVSQLPPMPDKHPPPFQLSQDDLLVIEQYFDTRVAAPPYRPNSLHSICRLLNLPAQVLKDFVQIMRLDLKPELGGDQLKWTVQICLRMPPSAVPIVPSGNACVVMGRMKILFFLQITRIPYGAVIGVGKDWKDSPSLVLPIVYDIQTNVTQLAERTGQVISPTMTAASTLLRRFAEFNAQQNQCTLFPAITDLLTNLQLAAEMPQPPPNQSIGPPVGVGVGVGSSPNPMMPMQQLPQQVGPQGPVGPGGYPQMGPNPGGPQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
615	Phosphorylation	TEVDAEVSPHKRKSN CEECEECCCCCCCCC	16.88	22817900
1007	Phosphorylation	AVYRGRASHEDDNPL HHHCCCCCCCCCCCC	26.50	23607784
1015	Phosphorylation	HEDDNPLSPMGMEDN CCCCCCCCCCCCCCC	17.69	23607784
1027	Phosphorylation	EDNFGGPSSVAGVSA CCCCCCCCCCCCCCC	40.49	23607784
1028	Phosphorylation	DNFGGPSSVAGVSAG CCCCCCCCCCCCCCC	21.08	23607784
1038	Phosphorylation	GVSAGGSSPFLGTGM CCCCCCCCCCCCCCC	23.97	22817900
1171	Phosphorylation	PSPRPGQSPDHKSTG CCCCCCCCCCCCCCC	37.90	19429919

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MED14_DROME !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MED14_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MED14_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MED17_DROME	MED17	physical	22036573
CPSF6_DROME	CG7185	physical	22036573
MED11_DROME	MED11	physical	22036573
MED7_DROME	MED7	physical	22036573
MED30_DROME	MED30	physical	22036573
MED27_DROME	MED27	physical	22036573
MED4_DROME	MED4	physical	22036573
MED10_DROME	MED10	physical	22036573
Y1505_DROME	CG11505	physical	22036573
MED25_DROME	MED25	physical	22036573
MED24_DROME	MED24	physical	22036573
MED31_DROME	MED31	physical	22036573
Y2199_DROME	CG2199	physical	22036573

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of Drosophila melanogaster embryos.";
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
J. Proteome Res. 7:1675-1682(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-615 AND SER-1015, ANDMASS SPECTROMETRY.
PubMed: 18327897