dbPTM

M2K1_ARATH - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	M2K1_ARATH
UniProt AC	Q94A06
Protein Name	Mitogen-activated protein kinase kinase 1
Gene Name	MKK1
Organism	Arabidopsis thaliana (Mouse-ear cress).
Sequence Length	354
Subcellular Localization
Protein Description	MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H(2)O(2) production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination..
Protein Sequence	MNRGSLCPNPICLPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIKVIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQSFYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKDPRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLAN

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Phosphorylation	QSISKFLTQSGTFKD HHHHHHHHCCCCCCC	24.66	23776212
27	Phosphorylation	ISKFLTQSGTFKDGD HHHHHHCCCCCCCCC	34.27	23776212
29	Phosphorylation	KFLTQSGTFKDGDLR HHHHCCCCCCCCCEE	32.53	30291188
218	Phosphorylation	VSKILTSTSSLANSF HHHHHHCCHHHHHHC	19.66	16211390
220	Phosphorylation	KILTSTSSLANSFVG HHHHCCHHHHHHCCC	31.43	-
224	Phosphorylation	STSSLANSFVGTYPY CCHHHHHHCCCCCCC	18.72	16211390
228	Phosphorylation	LANSFVGTYPYMSPE HHHHCCCCCCCCCHH	18.93	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of M2K1_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
218	T	Phosphorylation	11875555
Phosphorylation at Thr-218 and Ser-224 by MAP kinase kinase kinases positively regulates kinase activity.
224	S	Phosphorylation	11875555
Phosphorylation at Thr-218 and Ser-224 by MAP kinase kinase kinases positively regulates kinase activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of M2K1_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
M3K1_ARATH	MEKK1	physical	9878570
MPK4_ARATH	MPK4	physical	9878570
M3K1_ARATH	MEKK1	physical	9804171
MPK4_ARATH	MPK4	physical	9804171
MPK4_ARATH	MPK4	physical	17059410

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of M2K1_ARATH

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"MAP kinase signalling cascade in Arabidopsis innate immunity."; Asai T., Tena G., Plotnikova J., Willmann M.R., Chiu W.-L.,Gomez-Gomez L., Boller T., Ausubel F.M., Sheen J.; Nature 415:977-983(2002). Cited for: PHOSPHORYLATION AT THR-218 AND SER-224, AND MUTAGENESIS OF THR-218 ANDSER-224.	11875555 [Abstract]