dbPTM

KCNN4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KCNN4_HUMAN
UniProt AC	O15554
Protein Name	Intermediate conductance calcium-activated potassium channel protein 4
Gene Name	KCNN4
Organism	Homo sapiens (Human).
Sequence Length	427
Subcellular Localization	Cell membrane Multi-pass membrane protein .
Protein Description	Forms a voltage-independent potassium channel that is activated by intracellular calcium. [PubMed: 26148990 Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin]
Protein Sequence	MGGDLVLGLGALRRRKRLLEQEKSLAGWALVLAGTGIGLMVLHAEMLWFGGCSWALYLFLVKCTISISTFLLLCLIVAFHAKEVQLFMTDNGLRDWRVALTGRQAAQIVLELVVCGLHPAPVRGPPCVQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
178	Phosphorylation	SGVLLNASYRSIGAL CCCCCCCCCCCCCCC	21.72	28348404
179	Phosphorylation	GVLLNASYRSIGALN CCCCCCCCCCCCCCH	13.13	23312004
199	Phosphorylation	HWFVAKLYMNTHPGR HHHHHHHHCCCCCHH	6.39	-
329	Phosphorylation	AWMFYKHTRRKESHA HHHHHHHHHHHHHHH	28.19	12029379
331	Ubiquitination	MFYKHTRRKESHAAR HHHHHHHHHHHHHHH	48.53	21890473
334	Phosphorylation	KHTRRKESHAARRHQ HHHHHHHHHHHHHHH	22.82	22817900
358	Phosphorylation	FRQVRLKHRKLREQV HHHHHHHHHHHHHHH	36.38	17157250
362	Ubiquitination	RLKHRKLREQVNSMV HHHHHHHHHHHHHHH	35.02	21890473
367	Phosphorylation	KLREQVNSMVDISKM HHHHHHHHHHHHHHH	22.71	25850435
394	Ubiquitination	SSHRALEKQIDTLAG HHHHHHHHHHHHHHH	54.10	23000965
398	Phosphorylation	ALEKQIDTLAGKLDA HHHHHHHHHHHHHHH	21.82	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
334	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
358	H	Phosphorylation		17157250
Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KCNN4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CALM_DROME	Cam	physical	10026195
RAB7A_HUMAN	RAB7A	physical	20720181
TS101_HUMAN	TSG101	physical	20720181

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB00257	Clotrimazole
DB00228	Enflurane
DB01159	Halothane
DB01110	Miconazole
DB00721	Procaine
DB00468	Quinine

Regulatory Network of KCNN4_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Histidine phosphorylation of the potassium channel KCa3.1 bynucleoside diphosphate kinase B is required for activation of KCa3.1and CD4 T cells."; Srivastava S., Li Z., Ko K., Choudhury P., Albaqumi M., Johnson A.K.,Yan Y., Backer J.M., Unutmaz D., Coetzee W.A., Skolnik E.Y.; Mol. Cell 24:665-675(2006). Cited for: FUNCTION, AND PHOSPHORYLATION AT HIS-358.	17157250 [Abstract]