KAP3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: KAP3_MOUSE
• UniProt AC: P31324
• Protein Name: cAMP-dependent protein kinase type II-beta regulatory subunit
• Gene Name: Prkar2b
• Organism: Mus musculus (Mouse).
• Sequence Length: 416
• Subcellular Localization: Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and at the cell membrane..
• Protein Description: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase..
• Protein Sequence: MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGAARFGHEGRTWGDAGAAAGGGIPSKGVNFAEEPMRSDSENGEEEEAAEAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQGDLADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCFRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSIEIPAGL ------CCCCCCCCH	29.00	20415495
71	Phosphorylation	AAGGGIPSKGVNFAE HCCCCCCCCCCCCCC	39.87	25338131
83	Phosphorylation	FAEEPMRSDSENGEE CCCCCCCCCCCCCCH	38.96	25521595
85	Phosphorylation	EEPMRSDSENGEEEE CCCCCCCCCCCCHHH	33.92	25521595
112	Phosphorylation	NRFTRRASVCAEAYN HHHHHHHHCHHHHHC	18.87	24925903
118	Phosphorylation	ASVCAEAYNPDEEED HHCHHHHHCCCCCCC	20.80	25521595
129	Phosphorylation	EEEDDAESRIIHPKT CCCCCHHHCCCCCCC	30.42	25619855
174	Ubiquitination	AMFEKLVKEGEHVID HHHHHHHHCCCCEEC	71.13	22790023
200	Phosphorylation	DRGTFDIYVKCDGVG ECCEEEEEEEECCCC	8.83	-
256	Acetylation	TFRRIIVKNNAKKRK EEEEEHHCCCHHHHH	33.79	-
256	Ubiquitination	TFRRIIVKNNAKKRK EEEEEHHCCCHHHHH	33.79	27667366
265	Phosphorylation	NAKKRKMYESFIESL CHHHHHHHHHHHHHC	16.08	29899451
267	Phosphorylation	KKRKMYESFIESLPF HHHHHHHHHHHHCHH	17.65	27180971
281	Phosphorylation	FLKSLEVSERLKVVD HHHHCCHHHCCEEEE	13.97	28059163
357	Ubiquitination	ELALVTNKPRAASAH EEEEEECCCCCCHHH	26.89	27667366
362	Phosphorylation	TNKPRAASAHAIGTV ECCCCCCHHHHHHHH	21.43	23684622
368	Phosphorylation	ASAHAIGTVKCLAMD CHHHHHHHHHHHHCC	15.64	25338131
396	Phosphorylation	IMKRNIATYEEQLVA HHHHHHCCHHHHHHH	27.89	29895711
397	Phosphorylation	MKRNIATYEEQLVAL HHHHHCCHHHHHHHH	14.40	29895711

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of KAP3_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of KAP3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of KAP3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KAPCA_HUMAN	PRKACA	physical	26496610
KAPCB_HUMAN	PRKACB	physical	26496610
KAP2_HUMAN	PRKAR2A	physical	26496610
MYOME_HUMAN	PDE4DIP	physical	26496610
AKAP9_HUMAN	AKAP9	physical	26496610
CK5P2_HUMAN	CDK5RAP2	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large scale localization of protein phosphorylation by use ofelectron capture dissociation mass spectrometry.";
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
Mol. Cell. Proteomics 8:904-912(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASSSPECTROMETRY.
PubMed: 19131326

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASSSPECTROMETRY.
PubMed: 19367708

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASSSPECTROMETRY.
PubMed: 17114649

"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry.";
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
J. Proteome Res. 6:250-262(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASSSPECTROMETRY.
PubMed: 17203969

"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations.";
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.;
Mol. Cell. Proteomics 5:914-922(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASSSPECTROMETRY.
PubMed: 16452087