dbPTM

IRS1_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	IRS1_RAT
UniProt AC	P35570
Protein Name	Insulin receptor substrate 1
Gene Name	Irs1
Organism	Rattus norvegicus (Rat).
Sequence Length	1235
Subcellular Localization
Protein Description	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit..
Protein Sequence	MASPPDTDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKAHHDGAGGGCGGSCSGSSGVGEAGEDLSYDTGPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPRTKSQSSSSCSNPISVPLRRHHLNNPPPSQVGLTRRSRTESITATSPASMVGGKPGSFRVRASSDGEGTMSRPASVDGSPVSPSTNRTHAHRHRGSSRLHPPLNHSRSIPMPSSRCSPSATSPVSLSSSSTSGHGSTSDCLFPRRSSASVSGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPARGEEELSNYICMGGKGASTLTAPNGHYILSRGGNGHRYIPGATMGTSPALTGDEAAGAADLDNRFRKRTHSAGTSPTISHQKTPSQSSVVSIEEYTEMMPAAYPPGGGSGGRLPGYRHSAFVPTHSYPEEGLEMHHLERRGGHHRPDSSNLHTDDGYMPMSPGVAPVPSNRKGNGDYMPMSPKSVSAPQQIINPIRRHPQRVDPNGYMMMSPSGSCSPDIGGGSCSSSSISAAPSGSSYGKPWTNGVGGHHTHALPHAKPPVESGGGKLLPCTGDYMNMSPVGDSNTSSPSECYYGPEDPQHKPVLSYYSLPRSFKHTQRPGEPEEGARHQHLRLSSSSGRLRYTATAEDSSSSTSSDSLGGGYCGARPESSVTHPHHHALQPHLPRKVDTAAQTNSRLARPTRLSLGDPKASTLPRVREQQQQQQQQQQSSLHPPEPKSPGEYVNIEFGSGQPGYLAGPATSRSSPSVRCLPQLHPAPREETGSEEYMNMDLGPGRRATWQESGGVELGRVGPAPPGAASICRPTRSVPNSRGDYMTMQIGCPRQSYVDTSPVAPVSYADMRTGIAAEKVSLPRTTGAAPPPSSTASASASVTPQGAAEQAAHSSLLGGPQGPGGMSAFTRVNLSPNHNQSAKVIRADTQGCRRRHSSETFSAPTRAANTVSFGAGAAGGGSGGGSEDVKRHSSASFENVWLRPGDLGGASKESAPGCGAAGGLEKSLNYIDLDLVKDVKQHPQDCPSQQQSLPPPPPHQPLGSNEGSSPRRSSEDLSTYASINFQKQPEDRQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MASPPDTDGF -----CCCCCCCCCC	49.15	27097102
7	Phosphorylation	-MASPPDTDGFSDVR -CCCCCCCCCCCCCC	43.74	23984901
11	Phosphorylation	PPDTDGFSDVRKVGY CCCCCCCCCCCCCCE	40.50	25575281
24	Phosphorylation	GYLRKPKSMHKRFFV CEECCCCCHHHHHHH	34.35	22817900
99	Phosphorylation	HFAIAADSEAEQDSW CEEEECCCHHHHCHH	33.70	22817900
265	Phosphorylation	EFRPRTKSQSSSSCS HCCCCCCCCCCCCCC	34.38	29779826
267	Phosphorylation	RPRTKSQSSSSCSNP CCCCCCCCCCCCCCC	39.00	27097102
268	Phosphorylation	PRTKSQSSSSCSNPI CCCCCCCCCCCCCCC	20.73	23984901
269	Phosphorylation	RTKSQSSSSCSNPIS CCCCCCCCCCCCCCC	40.38	27097102
270	Phosphorylation	TKSQSSSSCSNPISV CCCCCCCCCCCCCCC	24.42	27097102
272	Phosphorylation	SQSSSSCSNPISVPL CCCCCCCCCCCCCCC	47.81	27097102
298	Phosphorylation	QVGLTRRSRTESITA HCCCCCCCCCCCEEE	40.02	25575281
300	Phosphorylation	GLTRRSRTESITATS CCCCCCCCCCEEECC	35.02	25575281
302	Phosphorylation	TRRSRTESITATSPA CCCCCCCCEEECCCH	25.32	22817900
304	Phosphorylation	RSRTESITATSPASM CCCCCCEEECCCHHH	32.91	27097102
306	Phosphorylation	RTESITATSPASMVG CCCCEEECCCHHHHC	26.96	27097102
307	Phosphorylation	TESITATSPASMVGG CCCEEECCCHHHHCC	18.92	14556646
310	Phosphorylation	ITATSPASMVGGKPG EEECCCHHHHCCCCC	19.86	27097102
315	Acetylation	PASMVGGKPGSFRVR CHHHHCCCCCEEEEE	40.03	22902405
318	Phosphorylation	MVGGKPGSFRVRASS HHCCCCCEEEEEECC	20.57	29779826
324	Phosphorylation	GSFRVRASSDGEGTM CEEEEEECCCCCCCC	20.56	27097102
325	Phosphorylation	SFRVRASSDGEGTMS EEEEEECCCCCCCCC	48.96	21738781
330	Phosphorylation	ASSDGEGTMSRPASV ECCCCCCCCCCCCCC	13.99	27097102
332	Phosphorylation	SDGEGTMSRPASVDG CCCCCCCCCCCCCCC	34.64	27097102
336	Phosphorylation	GTMSRPASVDGSPVS CCCCCCCCCCCCCCC	24.35	23298284
340	Phosphorylation	RPASVDGSPVSPSTN CCCCCCCCCCCCCCC	20.05	27097102
343	Phosphorylation	SVDGSPVSPSTNRTH CCCCCCCCCCCCCCC	19.27	27097102
345	Phosphorylation	DGSPVSPSTNRTHAH CCCCCCCCCCCCCCC	30.70	27097102
346	Phosphorylation	GSPVSPSTNRTHAHR CCCCCCCCCCCCCCC	31.91	27097102
357	Phosphorylation	HAHRHRGSSRLHPPL CCCCCCCCCCCCCCC	16.55	22817900
358	Phosphorylation	AHRHRGSSRLHPPLN CCCCCCCCCCCCCCC	41.50	22817900
369	Phosphorylation	PPLNHSRSIPMPSSR CCCCCCCCCCCCCCC	33.77	22817900
378	Phosphorylation	PMPSSRCSPSATSPV CCCCCCCCCCCCCCC	22.30	27097102
380	Phosphorylation	PSSRCSPSATSPVSL CCCCCCCCCCCCCCC	28.08	27097102
382	Phosphorylation	SRCSPSATSPVSLSS CCCCCCCCCCCCCCC	37.59	27097102
383	Phosphorylation	RCSPSATSPVSLSSS CCCCCCCCCCCCCCC	24.03	27097102
386	Phosphorylation	PSATSPVSLSSSSTS CCCCCCCCCCCCCCC	26.39	27097102
388	Phosphorylation	ATSPVSLSSSSTSGH CCCCCCCCCCCCCCC	22.22	27097102
389	Phosphorylation	TSPVSLSSSSTSGHG CCCCCCCCCCCCCCC	33.33	27097102
390	Phosphorylation	SPVSLSSSSTSGHGS CCCCCCCCCCCCCCC	33.97	27097102
391	Phosphorylation	PVSLSSSSTSGHGST CCCCCCCCCCCCCCC	28.65	27097102
392	Phosphorylation	VSLSSSSTSGHGSTS CCCCCCCCCCCCCCC	40.45	27097102
393	Phosphorylation	SLSSSSTSGHGSTSD CCCCCCCCCCCCCCC	30.64	27097102
397	Phosphorylation	SSTSGHGSTSDCLFP CCCCCCCCCCCCCCC	21.04	27097102
398	Phosphorylation	STSGHGSTSDCLFPR CCCCCCCCCCCCCCC	32.93	27097102
399	Phosphorylation	TSGHGSTSDCLFPRR CCCCCCCCCCCCCCC	28.20	27097102
407	Phosphorylation	DCLFPRRSSASVSGS CCCCCCCCCCCCCCC	31.38	22817900
408	Phosphorylation	CLFPRRSSASVSGSP CCCCCCCCCCCCCCC	23.81	22817900
410	Phosphorylation	FPRRSSASVSGSPSD CCCCCCCCCCCCCCC	20.82	23984901
412	Phosphorylation	RRSSASVSGSPSDGG CCCCCCCCCCCCCCC	30.93	23984901
414	Phosphorylation	SSASVSGSPSDGGFI CCCCCCCCCCCCCCC	17.45	29779826
416	Phosphorylation	ASVSGSPSDGGFISS CCCCCCCCCCCCCCC	51.05	23984901
422	Phosphorylation	PSDGGFISSDEYGSS CCCCCCCCCCCCCCC	29.49	28689409
423	Phosphorylation	SDGGFISSDEYGSSP CCCCCCCCCCCCCCC	29.64	28689409
426	Phosphorylation	GFISSDEYGSSPCDF CCCCCCCCCCCCCCC	27.57	28689409
428	Phosphorylation	ISSDEYGSSPCDFRS CCCCCCCCCCCCCHH	30.15	28689409
429	Phosphorylation	SSDEYGSSPCDFRSS CCCCCCCCCCCCHHH	25.78	28689409
439	Phosphorylation	DFRSSFRSVTPDSLG CCHHHHHCCCHHHCC	28.47	27097102
441	Phosphorylation	RSSFRSVTPDSLGHT HHHHHCCCHHHCCCC	23.71	27097102
444	Phosphorylation	FRSVTPDSLGHTPPA HHCCCHHHCCCCCCC	37.14	27097102
448	Phosphorylation	TPDSLGHTPPARGEE CHHHCCCCCCCCCCH	29.21	27097102
458	Phosphorylation	ARGEEELSNYICMGG CCCCHHHHCCEECCC	30.76	23984901
460	Phosphorylation	GEEELSNYICMGGKG CCHHHHCCEECCCCC	7.66	22817900
489	Nitration	RGGNGHRYIPGATMG CCCCCCCCCCCCCCC	13.04	-
489	Phosphorylation	RGGNGHRYIPGATMG CCCCCCCCCCCCCCC	13.04	28689409
494	Phosphorylation	HRYIPGATMGTSPAL CCCCCCCCCCCCCCC	23.27	23298284
497	Phosphorylation	IPGATMGTSPALTGD CCCCCCCCCCCCCCC	22.25	28689409
498	Phosphorylation	PGATMGTSPALTGDE CCCCCCCCCCCCCCH	11.49	22817900
502	Phosphorylation	MGTSPALTGDEAAGA CCCCCCCCCCHHHCC	44.70	22817900
520	Phosphorylation	DNRFRKRTHSAGTSP CHHHHHHHHCCCCCC	24.18	25575281
522	Phosphorylation	RFRKRTHSAGTSPTI HHHHHHHCCCCCCCC	27.99	23298284
525	Phosphorylation	KRTHSAGTSPTISHQ HHHHCCCCCCCCCCC	31.11	27097102
526	Phosphorylation	RTHSAGTSPTISHQK HHHCCCCCCCCCCCC	21.09	21738781
528	Phosphorylation	HSAGTSPTISHQKTP HCCCCCCCCCCCCCC	34.57	27097102
570	Phosphorylation	RLPGYRHSAFVPTHS CCCCCCCCCCCCCCC	17.88	22817900
575	Phosphorylation	RHSAFVPTHSYPEEG CCCCCCCCCCCCCCC	20.39	25575281
577	Phosphorylation	SAFVPTHSYPEEGLE CCCCCCCCCCCCCCC	44.88	25575281
578	Phosphorylation	AFVPTHSYPEEGLEM CCCCCCCCCCCCCCC	13.68	25575281
599	Phosphorylation	GGHHRPDSSNLHTDD CCCCCCCCCCCCCCC	24.65	25575281
600	Phosphorylation	GHHRPDSSNLHTDDG CCCCCCCCCCCCCCC	51.28	25575281
604	Phosphorylation	PDSSNLHTDDGYMPM CCCCCCCCCCCCCCC	38.66	25575281
608	Phosphorylation	NLHTDDGYMPMSPGV CCCCCCCCCCCCCCC	12.68	22817900
612	Phosphorylation	DDGYMPMSPGVAPVP CCCCCCCCCCCCCCC	16.78	16354680
628	Phosphorylation	NRKGNGDYMPMSPKS CCCCCCCCCCCCHHC	12.43	22817900
632	Phosphorylation	NGDYMPMSPKSVSAP CCCCCCCCHHCCCCC	24.25	23298284
635	Phosphorylation	YMPMSPKSVSAPQQI CCCCCHHCCCCCHHH	25.37	16354680
637	Phosphorylation	PMSPKSVSAPQQIIN CCCHHCCCCCHHHCC	41.12	-
658	Phosphorylation	QRVDPNGYMMMSPSG CCCCCCCCEEECCCC	6.62	11572799
662	Phosphorylation	PNGYMMMSPSGSCSP CCCCEEECCCCCCCC	9.68	-
727	Phosphorylation	LLPCTGDYMNMSPVG EEECCCCCCCCCCCC	7.54	11572799
731	Phosphorylation	TGDYMNMSPVGDSNT CCCCCCCCCCCCCCC	16.07	-
761	Phosphorylation	KPVLSYYSLPRSFKH CCCEEEEECCCCCCC	24.54	23984901
789	Phosphorylation	QHLRLSSSSGRLRYT CEEEEECCCCCEEEE	33.17	22817900
857	Phosphorylation	LARPTRLSLGDPKAS CCCCCCCCCCCCCHH	27.32	30181290
891	Phosphorylation	LHPPEPKSPGEYVNI CCCCCCCCCCCEEEE	49.18	29779826
895	Phosphorylation	EPKSPGEYVNIEFGS CCCCCCCEEEEECCC	12.52	23298284
902	Phosphorylation	YVNIEFGSGQPGYLA EEEEECCCCCCCEEC	39.31	23984901
914	O-linked_Glycosylation	YLAGPATSRSSPSVR EECCCCCCCCCCCCC	32.07	19671924
939	Nitration	EETGSEEYMNMDLGP HHCCCHHHCCCCCCC	6.96	-
939	Phosphorylation	EETGSEEYMNMDLGP HHCCCHHHCCCCCCC	6.96	22817900
987	Phosphorylation	VPNSRGDYMTMQIGC CCCCCCCEEEEEEEC	9.41	22817900
998	Phosphorylation	QIGCPRQSYVDTSPV EEECCCCCCCCCCCC	28.53	28689409
999	Nitration	IGCPRQSYVDTSPVA EECCCCCCCCCCCCC	8.26	-
999	Phosphorylation	IGCPRQSYVDTSPVA EECCCCCCCCCCCCC	8.26	28689409
1002	Phosphorylation	PRQSYVDTSPVAPVS CCCCCCCCCCCCCCC	25.53	23984901
1003	Phosphorylation	RQSYVDTSPVAPVSY CCCCCCCCCCCCCCH	16.95	23298284
1009	O-linked_Glycosylation	TSPVAPVSYADMRTG CCCCCCCCHHHHCCC	17.34	19719639
1009	Phosphorylation	TSPVAPVSYADMRTG CCCCCCCCHHHHCCC	17.34	25575281
1010	Nitration	SPVAPVSYADMRTGI CCCCCCCHHHHCCCC	13.57	-
1010	Phosphorylation	SPVAPVSYADMRTGI CCCCCCCHHHHCCCC	13.57	25575281
1036	O-linked_Glycosylation	GAAPPPSSTASASAS CCCCCCCCCCCCCCC	33.49	19671924
1041	O-linked_Glycosylation	PSSTASASASVTPQG CCCCCCCCCCCCCCC	20.69	19671924
1041	Phosphorylation	PSSTASASASVTPQG CCCCCCCCCCCCCCC	20.69	-
1045	Phosphorylation	ASASASVTPQGAAEQ CCCCCCCCCCCHHHH	13.85	22817900
1077	Phosphorylation	AFTRVNLSPNHNQSA CCEEEECCCCCCCCC	20.60	29779826
1083	Phosphorylation	LSPNHNQSAKVIRAD CCCCCCCCCCEEEEC	35.06	27097102
1099	Phosphorylation	QGCRRRHSSETFSAP CCHHHHCCCCCCCCC	28.23	29779826
1100	Phosphorylation	GCRRRHSSETFSAPT CHHHHCCCCCCCCCC	34.23	28689409
1102	Phosphorylation	RRRHSSETFSAPTRA HHHCCCCCCCCCCCC	25.49	27097102
1104	Phosphorylation	RHSSETFSAPTRAAN HCCCCCCCCCCCCCC	40.53	23298284
1107	Phosphorylation	SETFSAPTRAANTVS CCCCCCCCCCCCCEE	32.43	26437020
1135	Phosphorylation	SEDVKRHSSASFENV CHHHHHHCCCCEEEE	31.47	27097102
1136	Phosphorylation	EDVKRHSSASFENVW HHHHHHCCCCEEEEE	23.08	27097102
1138	Phosphorylation	VKRHSSASFENVWLR HHHHCCCCEEEEEEC	34.73	27097102
1153	Phosphorylation	PGDLGGASKESAPGC CCCCCCCCCCCCCCC	39.98	27097102
1169	Phosphorylation	AAGGLEKSLNYIDLD CCCCHHHHCCCCCHH	16.69	23984901
1172	Nitration	GLEKSLNYIDLDLVK CHHHHCCCCCHHHHH	10.91	-
1172	Phosphorylation	GLEKSLNYIDLDLVK CHHHHCCCCCHHHHH	10.91	22817900
1194	Phosphorylation	DCPSQQQSLPPPPPH CCCCHHCCCCCCCCC	38.21	27097102
1206	Phosphorylation	PPHQPLGSNEGSSPR CCCCCCCCCCCCCCC	39.12	27097102
1210	Phosphorylation	PLGSNEGSSPRRSSE CCCCCCCCCCCCCCC	30.87	27097102
1211	Phosphorylation	LGSNEGSSPRRSSED CCCCCCCCCCCCCCH	33.03	27097102
1215	Phosphorylation	EGSSPRRSSEDLSTY CCCCCCCCCCHHHHH	39.08	23298284
1216	Phosphorylation	GSSPRRSSEDLSTYA CCCCCCCCCHHHHHH	33.06	23298284
1220	Phosphorylation	RRSSEDLSTYASINF CCCCCHHHHHHHCCC	31.52	25575281
1221	Phosphorylation	RSSEDLSTYASINFQ CCCCHHHHHHHCCCC	30.33	25575281
1222	Phosphorylation	SSEDLSTYASINFQK CCCHHHHHHHCCCCC	8.58	11018022

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
24	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
24	S	Phosphorylation	Kinase	IRAK1	B2RYH5	GPS
24	S	Phosphorylation	Kinase	PRKCA	P20444	GPS
24	S	Phosphorylation	Kinase	PKCA	P17252	PSP
24	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
24	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
99	S	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
99	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
99	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
99	S	Phosphorylation	Kinase	CK2	-	Uniprot
265	S	Phosphorylation	Kinase	RPS6KB1	P67999	Uniprot
302	S	Phosphorylation	Kinase	ROCK1	Q63644	PSP
302	S	Phosphorylation	Kinase	RPS6KB1	P67999	Uniprot
307	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
307	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
307	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
307	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
307	S	Phosphorylation	Kinase	JNK1	P49185	PSP
307	S	Phosphorylation	Kinase	RPS6KB1	P67999	Uniprot
307	S	Phosphorylation	Kinase	IKKB	Q9QY78	Uniprot
307	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
318	S	Phosphorylation	Kinase	PKCZ	Q05513	PSP
318	S	Phosphorylation	Kinase	PRKCZ	P09217	GPS
332	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
336	S	Phosphorylation	Kinase	GSK-FAMILY	-	GPS
357	S	Phosphorylation	Kinase	PRKCD	P09215	GPS
422	S	Phosphorylation	Kinase	MTOR	P42346	PSP
423	S	Phosphorylation	Kinase	MTOR	P42346	PSP
460	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
498	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
502	T	Phosphorylation	Kinase	CK2	-	Uniprot
502	T	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
502	T	Phosphorylation	Kinase	CK2_GROUP	-	PhosphoELM
502	T	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
522	S	Phosphorylation	Kinase	AKT1	P47196	PSP
522	S	Phosphorylation	Kinase	RPS6KB1	P67999	Uniprot
570	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
608	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
608	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
612	S	Phosphorylation	Kinase	PKCA	P05696	PSP
612	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
612	S	Phosphorylation	Kinase	PRKCZ	Q05513	GPS
612	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
612	S	Phosphorylation	Kinase	CK2-FAMILY	-	GPS
612	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
628	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
628	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
632	S	Phosphorylation	Kinase	RPS6KB1	P67999	Uniprot
632	S	Phosphorylation	Kinase	MTOR	P42345	PSP
632	S	Phosphorylation	Kinase	ROCK1	Q63644	PSP
632	S	Phosphorylation	Kinase	ROCK2	Q62868	Uniprot
635	S	Phosphorylation	Kinase	MTOR	P42345	PSP
635	S	Phosphorylation	Kinase	ROCK1	Q63644	PSP
658	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
727	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
789	S	Phosphorylation	Kinase	AMPK	Q09137	Uniprot
789	S	Phosphorylation	Kinase	AMPK_GROUP	-	PhosphoELM
789	S	Phosphorylation	Kinase	SIK2	-	Uniprot
789	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
789	S	Phosphorylation	Kinase	QIK	Q8CFH6	PSP
789	S	Phosphorylation	Kinase	SIK2	Q9H0K1	GPS
895	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
895	Y	Phosphorylation	Kinase	FYN	P39688	PSP
895	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
939	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
939	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
987	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
987	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
1100	S	Phosphorylation	Kinase	RPS6KB1	Q8BSK8	GPS
1100	S	Phosphorylation	Kinase	RPS6KB1	P67999	Uniprot
1172	Y	Phosphorylation	Kinase	IGF1R	P24062	PhosphoELM
1172	Y	Phosphorylation	Kinase	FYN	P39688	PSP
1172	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot
1222	Y	Phosphorylation	Kinase	INSR	P15127	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
265	S	Phosphorylation	12006586
Phosphorylated at Ser-265, Ser-302, Ser-632 and Ser-1100 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (By similarity).
302	S	Phosphorylation	12006586
Phosphorylated at Ser-265, Ser-302, Ser-632 and Ser-1100 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (By similarity).
307	S	Phosphorylation	11606564
Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats.
632	S	Phosphorylation	12006586
Phosphorylated at Ser-265, Ser-302, Ser-632 and Ser-1100 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (By similarity).
789	S	Phosphorylation	12006586
Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats.
1100	S	Phosphorylation	12006586
Phosphorylated at Ser-265, Ser-302, Ser-632 and Ser-1100 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of IRS1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
P85A_RAT	Pik3r1	physical	12594288
UBC_RAT	Ubc	physical	15456867
GRB2_RAT	Grb2	physical	12960006
P85A_RAT	Pik3r1	physical	12960006
SQSTM_RAT	Sqstm1	physical	22761437
P85A_RAT	Pik3r1	physical	8628286

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of IRS1_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"In vivo phosphorylation of insulin receptor substrate 1 at serine 789by a novel serine kinase in insulin-resistant rodents."; Qiao L.Y., Zhande R., Jetton T.L., Zhou G., Sun X.-J.; J. Biol. Chem. 277:26530-26539(2002). Cited for: PHOSPHORYLATION AT SER-789.	12006586 [Abstract]
"Phosphorylation of Ser307 in insulin receptor substrate-1 blocksinteractions with the insulin receptor and inhibits insulin action."; Aguirre V., Werner E.D., Giraud J., Lee Y.H., Shoelson S.E.,White M.F.; J. Biol. Chem. 277:1531-1537(2002). Cited for: PHOSPHORYLATION AT SER-307.	11606564 [Abstract]
"Phosphorylation of the insulin receptor substrate IRS-1 by caseinkinase II."; Tanasijevic M.J., Myers M.G. Jr., Thoma R.S., Crimmins D.L.,White M.F., Sacks D.B.; J. Biol. Chem. 268:18157-18166(1993). Cited for: PHOSPHORYLATION AT SER-99 AND THR-502.	8349691 [Abstract]
"Pleiotropic insulin signals are engaged by multisite phosphorylationof IRS-1."; Sun X.-J., Crimmins D.L., Myers M.G. Jr., Miralpeix M., White M.F.; Mol. Cell. Biol. 13:7418-7428(1993). Cited for: PHOSPHORYLATION AT TYR-460; TYR-608; TYR-895; TYR-939; TYR-987;TYR-1172 AND TYR-1222.	7504175 [Abstract]

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