IRAK4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IRAK4_MOUSE
• UniProt AC: Q8R4K2
• Protein Name: Interleukin-1 receptor-associated kinase 4
• Gene Name: Irak4
• Organism: Mus musculus (Mouse).
• Sequence Length: 459
• Subcellular Localization: Cytoplasm.
• Protein Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections (By similarity)..
• Protein Sequence: MNKPLTPSTYIRNLNVGILRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTCELLFDWGTTNCTVGDLVDLLVQIELFAPATLLLPDAVPQTVKSLPPREAATVAQTHGPCQEKDRTSVMPMPKLEHSCEPPDSSSPDNRSVESSDTRFHSFSFHELKSITNNFDEQPASAGGNRMGEGGFGVVYKGCVNNTIVAVKKLGAMVEISTEELKQQFDQEIKVMATCQHENLVELLGFSSDSDNLCLVYAYMPNGSLLDRLSCLDGTPPLSWHTRCKVAQGTANGIRFLHENHHIHRDIKSANILLDKDFTAKISDFGLARASARLAQTVMTSRIVGTTAYMAPEALRGEITPKSDIYSFGVVLLELITGLAAVDENREPQLLLDIKEEIEDEEKTIEDYTDEKMSDADPASVEAMYSAASQCLHEKKNRRPDIAKVQQLLQEMSA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MNKPLTPS -------CCCCCCHH	15.24	-
6	Phosphorylation	--MNKPLTPSTYIRN --CCCCCCHHHHHHH	24.58	29472430
8	Phosphorylation	MNKPLTPSTYIRNLN CCCCCCHHHHHHHCC	28.71	29472430
9	Phosphorylation	NKPLTPSTYIRNLNV CCCCCHHHHHHHCCH	24.95	29472430
34	Acetylation	DPQEGWKKLAVAIKK CHHHCHHHHEEEEEC	34.86	-
43	Phosphorylation	AVAIKKPSGDDRYNQ EEEEECCCCCCHHHH	64.83	29109428
144	Phosphorylation	PMPKLEHSCEPPDSS CCCCCCCCCCCCCCC	15.70	25619855
145	Glutathionylation	MPKLEHSCEPPDSSS CCCCCCCCCCCCCCC	11.11	24333276
150	Phosphorylation	HSCEPPDSSSPDNRS CCCCCCCCCCCCCCC	38.19	25619855
151	Phosphorylation	SCEPPDSSSPDNRSV CCCCCCCCCCCCCCC	53.95	25619855
152	Phosphorylation	CEPPDSSSPDNRSVE CCCCCCCCCCCCCCC	39.98	25619855
342	Phosphorylation	ASARLAQTVMTSRIV HHHHHHHHHHHHCCC	13.46	30635358
345	Phosphorylation	RLAQTVMTSRIVGTT HHHHHHHHHCCCCCC	15.54	30635358
346	Phosphorylation	LAQTVMTSRIVGTTA HHHHHHHHCCCCCCC	11.25	30635358
351	Phosphorylation	MTSRIVGTTAYMAPE HHHCCCCCCCCCCHH	9.43	30635358
352	Phosphorylation	TSRIVGTTAYMAPEA HHCCCCCCCCCCHHH	15.17	30635358
354	Phosphorylation	RIVGTTAYMAPEALR CCCCCCCCCCHHHHC	7.54	30635358

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of IRAK4_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IRAK4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IRAK4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MYD88_MOUSE	Myd88	physical	19378012
TIRAP_MOUSE	Tirap	physical	19378012
IRAK1_MOUSE	Irak1	physical	19378012

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.