dbPTM

ICAL_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ICAL_HUMAN
UniProt AC	P20810
Protein Name	Calpastatin
Gene Name	CAST
Organism	Homo sapiens (Human).
Sequence Length	708
Subcellular Localization
Protein Description	Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue..
Protein Sequence	MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2 (in isoform 10)	Phosphorylation	-	52.80	26055452
2 (in isoform 5)	Phosphorylation	-	52.80	26055452
2 (in isoform 6)	Phosphorylation	-	52.80	26055452
2 (in isoform 7)	Phosphorylation	-	52.80	26055452
2 (in isoform 9)	Phosphorylation	-	52.80	26055452
11 (in isoform 10)	Phosphorylation	-	7.06	22167270
11 (in isoform 5)	Phosphorylation	-	7.06	22167270
11 (in isoform 6)	Phosphorylation	-	7.06	22167270
11 (in isoform 7)	Phosphorylation	-	7.06	22167270
11 (in isoform 9)	Phosphorylation	-	7.06	22167270
15	Sulfoxidation	AIPVSQQMEGPHLPN CCCHHHHCCCCCCCC	5.08	30846556
23	Malonylation	EGPHLPNKKKHKKQA CCCCCCCCHHHHHHH	62.35	26320211
30 (in isoform 4)	Phosphorylation	-	17.41	25849741
31 (in isoform 10)	Phosphorylation	-	7.95	20068231
31 (in isoform 5)	Phosphorylation	-	7.95	20068231
31 (in isoform 6)	Phosphorylation	-	7.95	20068231
31 (in isoform 7)	Phosphorylation	-	7.95	20068231
31 (in isoform 9)	Phosphorylation	-	7.95	20068231
32	Sumoylation	KHKKQAVKTEPEKKS HHHHHHCCCCCCHHC	50.62	-
32	Sumoylation	KHKKQAVKTEPEKKS HHHHHHCCCCCCHHC	50.62	28112733
32 (in isoform 10)	Phosphorylation	-	50.62	20068231
32 (in isoform 5)	Phosphorylation	-	50.62	20068231
32 (in isoform 6)	Phosphorylation	-	50.62	20068231
32 (in isoform 7)	Phosphorylation	-	50.62	20068231
32 (in isoform 9)	Phosphorylation	-	50.62	20068231
34 (in isoform 10)	Phosphorylation	-	45.83	25159151
34 (in isoform 5)	Phosphorylation	-	45.83	25159151
34 (in isoform 6)	Phosphorylation	-	45.83	25159151
34 (in isoform 7)	Phosphorylation	-	45.83	25159151
34 (in isoform 9)	Phosphorylation	-	45.83	25159151
36 (in isoform 10)	Phosphorylation	-	67.87	20068231
36 (in isoform 5)	Phosphorylation	-	67.87	20068231
36 (in isoform 6)	Phosphorylation	-	67.87	20068231
36 (in isoform 7)	Phosphorylation	-	67.87	20068231
36 (in isoform 9)	Phosphorylation	-	67.87	20068231
39	Phosphorylation	KTEPEKKSQSTKLSV CCCCCHHCCCCCCEE	40.19	23312004
39 (in isoform 2)	Phosphorylation	-	40.19	24719451
41	Phosphorylation	EPEKKSQSTKLSVVH CCCHHCCCCCCEEEE	34.30	28258704
42	Phosphorylation	PEKKSQSTKLSVVHE CCHHCCCCCCEEEEC	28.50	29214152
43	Acetylation	EKKSQSTKLSVVHEK CHHCCCCCCEEEECC	43.97	25953088
45	Phosphorylation	KSQSTKLSVVHEKKS HCCCCCCEEEECCCC	24.53	28857561
45 (in isoform 2)	Phosphorylation	-	24.53	27251275
48 (in isoform 5)	Methylation	-	38.72	-
50	Acetylation	KLSVVHEKKSQEGKP CCEEEECCCCCCCCC	42.73	-
51	Methylation	LSVVHEKKSQEGKPK CEEEECCCCCCCCCC	55.15	-
51 (in isoform 10)	Phosphorylation	-	55.15	25159151
51 (in isoform 5)	Phosphorylation	-	55.15	25159151
51 (in isoform 6)	Phosphorylation	-	55.15	25159151
51 (in isoform 7)	Phosphorylation	-	55.15	25159151
51 (in isoform 9)	Phosphorylation	-	55.15	25159151
52	Phosphorylation	SVVHEKKSQEGKPKE EEEECCCCCCCCCCC	44.52	26657352
52 (in isoform 2)	Phosphorylation	-	44.52	24719451
54 (in isoform 10)	Phosphorylation	-	76.03	28985074
54 (in isoform 5)	Phosphorylation	-	76.03	28985074
54 (in isoform 6)	Phosphorylation	-	76.03	28985074
54 (in isoform 7)	Phosphorylation	-	76.03	28985074
54 (in isoform 9)	Phosphorylation	-	76.03	25159151
55 (in isoform 10)	Phosphorylation	-	43.88	25159151
55 (in isoform 5)	Phosphorylation	-	43.88	25159151
55 (in isoform 6)	Phosphorylation	-	43.88	25159151
55 (in isoform 7)	Phosphorylation	-	43.88	25159151
55 (in isoform 9)	Phosphorylation	-	43.88	25159151
56	Methylation	EKKSQEGKPKEHTEP CCCCCCCCCCCCCCC	52.76	-
57 (in isoform 10)	Phosphorylation	-	59.11	25159151
57 (in isoform 5)	Phosphorylation	-	59.11	25159151
57 (in isoform 6)	Phosphorylation	-	59.11	25159151
57 (in isoform 7)	Phosphorylation	-	59.11	25159151
57 (in isoform 9)	Phosphorylation	-	59.11	25159151
58	Methylation	KSQEGKPKEHTEPKS CCCCCCCCCCCCCCC	66.66	-
58 (in isoform 10)	Phosphorylation	-	66.66	25159151
58 (in isoform 5)	Phosphorylation	-	66.66	25159151
58 (in isoform 6)	Phosphorylation	-	66.66	25159151
58 (in isoform 7)	Phosphorylation	-	66.66	25159151
58 (in isoform 9)	Phosphorylation	-	66.66	25159151
60 (in isoform 10)	Phosphorylation	-	41.48	28152594
60 (in isoform 5)	Phosphorylation	-	41.48	28152594
60 (in isoform 6)	Phosphorylation	-	41.48	28152594
60 (in isoform 7)	Phosphorylation	-	41.48	28152594
60 (in isoform 9)	Phosphorylation	-	41.48	28152594
61 (in isoform 10)	Phosphorylation	-	55.89	28152594
61 (in isoform 5)	Phosphorylation	-	55.89	28152594
61 (in isoform 6)	Phosphorylation	-	55.89	28152594
61 (in isoform 7)	Phosphorylation	-	55.89	28152594
61 (in isoform 9)	Phosphorylation	-	55.89	28152594
64	2-Hydroxyisobutyrylation	PKEHTEPKSLPKQAS CCCCCCCCCCCCCCC	59.39	-
65	Phosphorylation	KEHTEPKSLPKQASD CCCCCCCCCCCCCCC	61.70	-
65 (in isoform 10)	Phosphorylation	-	61.70	22199227
65 (in isoform 5)	Phosphorylation	-	61.70	22199227
65 (in isoform 6)	Phosphorylation	-	61.70	22199227
65 (in isoform 7)	Phosphorylation	-	61.70	22199227
65 (in isoform 9)	Phosphorylation	-	61.70	22199227
67 (in isoform 10)	Phosphorylation	-	33.53	22199227
67 (in isoform 5)	Phosphorylation	-	33.53	22199227
67 (in isoform 6)	Phosphorylation	-	33.53	22199227
67 (in isoform 7)	Phosphorylation	-	33.53	22199227
67 (in isoform 9)	Phosphorylation	-	33.53	22199227
69 (in isoform 10)	Phosphorylation	-	44.13	22199227
69 (in isoform 5)	Phosphorylation	-	44.13	22199227
69 (in isoform 6)	Phosphorylation	-	44.13	22199227
69 (in isoform 7)	Phosphorylation	-	44.13	22199227
69 (in isoform 9)	Phosphorylation	-	44.13	22199227
71	Phosphorylation	KSLPKQASDTGSNDA CCCCCCCCCCCCCHH	32.96	21955146
73	Phosphorylation	LPKQASDTGSNDAHN CCCCCCCCCCCHHHH	39.76	26699800
74 (in isoform 10)	Phosphorylation	-	29.46	24719451
74 (in isoform 5)	Phosphorylation	-	29.46	24719451
74 (in isoform 6)	Phosphorylation	-	29.46	24719451
74 (in isoform 7)	Phosphorylation	-	29.46	24719451
74 (in isoform 9)	Phosphorylation	-	29.46	24719451
75	Phosphorylation	KQASDTGSNDAHNKK CCCCCCCCCHHHHHH	33.36	20068231
80 (in isoform 5)	Methylation	-	49.60	-
80 (in isoform 6)	Methylation	-	49.60	-
81 (in isoform 10)	Phosphorylation	-	32.04	27251275
81 (in isoform 5)	Phosphorylation	-	32.04	27251275
81 (in isoform 6)	Phosphorylation	-	32.04	27251275
81 (in isoform 7)	Phosphorylation	-	32.04	27251275
81 (in isoform 9)	Phosphorylation	-	32.04	23911959
83 (in isoform 10)	Phosphorylation	-	13.21	27251275
83 (in isoform 5)	Phosphorylation	-	13.21	27251275
83 (in isoform 6)	Phosphorylation	-	13.21	27251275
83 (in isoform 7)	Phosphorylation	-	13.21	27251275
83 (in isoform 9)	Phosphorylation	-	13.21	27251275
85	Phosphorylation	AHNKKAVSRSAEQQP HHHHHHHHHCHHCCC	25.94	25056879
87	Phosphorylation	NKKAVSRSAEQQPSE HHHHHHHCHHCCCCC	28.75	20068231
87 (in isoform 10)	Phosphorylation	-	28.75	-
87 (in isoform 5)	Phosphorylation	-	28.75	-
87 (in isoform 9)	Phosphorylation	-	28.75	-
89 (in isoform 10)	Phosphorylation	-	51.50	-
89 (in isoform 5)	Phosphorylation	-	51.50	-
89 (in isoform 9)	Phosphorylation	-	51.50	-
93	Phosphorylation	RSAEQQPSEKSTEPK HCHHCCCCCCCCCCC	54.01	30242111
95	Acetylation	AEQQPSEKSTEPKTK HHCCCCCCCCCCCCC	68.00	26051181
96	Phosphorylation	EQQPSEKSTEPKTKP HCCCCCCCCCCCCCH	33.67	18669648
100 (in isoform 3)	Phosphorylation	-	64.05	23090842
101	Phosphorylation	EKSTEPKTKPQDMIS CCCCCCCCCHHHCHH	59.68	28857561
108	Phosphorylation	TKPQDMISAGGESVA CCHHHCHHCCCCCCC	17.77	20068231
113	Phosphorylation	MISAGGESVAGITAI CHHCCCCCCCCEEEE	22.12	20068231
113 (in isoform 9)	Phosphorylation	-	22.12	24719451
118	Phosphorylation	GESVAGITAISGKPG CCCCCCEEEECCCCC	19.61	24719451
118 (in isoform 3)	Phosphorylation	-	19.61	28450419
119 (in isoform 3)	Phosphorylation	-	7.63	28450419
121	Phosphorylation	VAGITAISGKPGDKK CCCEEEECCCCCCCH	38.00	20068231
122 (in isoform 3)	Phosphorylation	-	33.78	28450419
123	Acetylation	GITAISGKPGDKKKE CEEEECCCCCCCHHC	38.75	26051181
123	Malonylation	GITAISGKPGDKKKE CEEEECCCCCCCHHC	38.75	26320211
125 (in isoform 3)	Phosphorylation	-	63.96	23927012
127 (in isoform 3)	Phosphorylation	-	72.21	28450419
133	Phosphorylation	DKKKEKKSLTPAVPV CCHHCCCCCCCCCCC	48.85	23927012
135	Phosphorylation	KKEKKSLTPAVPVES HHCCCCCCCCCCCCC	19.12	23927012
135 (in isoform 7)	Phosphorylation	-	19.12	25849741
142	Phosphorylation	TPAVPVESKPDKPSG CCCCCCCCCCCCCCC	50.12	23927012
143	Malonylation	PAVPVESKPDKPSGK CCCCCCCCCCCCCCC	43.67	26320211
146	Malonylation	PVESKPDKPSGKSGM CCCCCCCCCCCCCCH	50.00	26320211
148	Phosphorylation	ESKPDKPSGKSGMDA CCCCCCCCCCCCHHH	65.67	23927012
150	Phosphorylation	KPDKPSGKSGMDAAL CCCCCCCCCCHHHHH	48.45	-
150 (in isoform 9)	Phosphorylation	-	48.45	24719451
155	Phosphorylation	SGKSGMDAALDDLID CCCCCHHHHHHHHHH	10.88	-
155 (in isoform 9)	Phosphorylation	-	10.88	24719451
160 (in isoform 9)	Phosphorylation	-	3.68	24719451
165	Acetylation	DDLIDTLGGPEETEE HHHHHHHCCCHHCCC	52.26	-
175	Phosphorylation	EETEEENTTYTGPEV HHCCCCCCCCCCCCC	25.18	-
175 (in isoform 9)	Phosphorylation	-	25.18	24719451
177	Phosphorylation	TEEENTTYTGPEVSD CCCCCCCCCCCCCCC	13.99	-
177 (in isoform 9)	Phosphorylation	-	13.99	27251275
178	Phosphorylation	EEENTTYTGPEVSDP CCCCCCCCCCCCCCC	44.14	1995645
183	Phosphorylation	TYTGPEVSDPMSSTY CCCCCCCCCCCCCHH	34.04	18452278
184	Phosphorylation	YTGPEVSDPMSSTYI CCCCCCCCCCCCHHH	48.36	17287340
187	Phosphorylation	PEVSDPMSSTYIEEL CCCCCCCCCHHHHHH	25.56	18452278
188	Phosphorylation	EVSDPMSSTYIEELG CCCCCCCCHHHHHHH	20.98	18452278
188	Ubiquitination	EVSDPMSSTYIEELG CCCCCCCCHHHHHHH	20.98	-
189	Phosphorylation	VSDPMSSTYIEELGK CCCCCCCHHHHHHHC	22.63	18452278
190	Phosphorylation	SDPMSSTYIEELGKR CCCCCCHHHHHHHCC	14.32	18452278
190 (in isoform 9)	Phosphorylation	-	14.32	27251275
200	Phosphorylation	ELGKREVTIPPKYRE HHHCCEECCCHHHHH	24.02	1995645
204	2-Hydroxyisobutyrylation	REVTIPPKYRELLAK CEECCCHHHHHHHHH	52.14	-
205	Phosphorylation	EVTIPPKYRELLAKK EECCCHHHHHHHHHH	18.33	28674419
205 (in isoform 2)	Phosphorylation	-	18.33	23090842
216	Phosphorylation	LAKKEGITGPPADSS HHHHCCCCCCCCCCC	55.26	23403867
222	Phosphorylation	ITGPPADSSKPIGPD CCCCCCCCCCCCCCC	42.22	23927012
223	Phosphorylation	TGPPADSSKPIGPDD CCCCCCCCCCCCCCH	42.89	23927012
223 (in isoform 2)	Phosphorylation	-	42.89	28450419
224 (in isoform 2)	Phosphorylation	-	44.60	28450419
225	Phosphorylation	PPADSSKPIGPDDAI CCCCCCCCCCCCHHH	38.42	-
227 (in isoform 2)	Phosphorylation	-	28.28	28450419
229	Phosphorylation	SSKPIGPDDAIDALS CCCCCCCCHHHHHHH	53.40	-
230	Phosphorylation	SKPIGPDDAIDALSS CCCCCCCHHHHHHHC	48.86	-
230 (in isoform 2)	Phosphorylation	-	48.86	23927012
231	Phosphorylation	KPIGPDDAIDALSSD CCCCCCHHHHHHHCC	14.67	-
232	Phosphorylation	PIGPDDAIDALSSDF CCCCCHHHHHHHCCC	4.02	-
232 (in isoform 2)	Phosphorylation	-	4.02	28450419
236	Phosphorylation	DDAIDALSSDFTCGS CHHHHHHHCCCCCCC	29.58	23927012
237	Phosphorylation	DAIDALSSDFTCGSP HHHHHHHCCCCCCCC	37.28	22167270
240	Phosphorylation	DALSSDFTCGSPTAA HHHHCCCCCCCCCCC	22.43	29255136
242	Phosphorylation	LSSDFTCGSPTAAGK HHCCCCCCCCCCCCC	33.33	1995645
243	Phosphorylation	SSDFTCGSPTAAGKK HCCCCCCCCCCCCCC	22.63	19664994
245	Phosphorylation	DFTCGSPTAAGKKTE CCCCCCCCCCCCCCC	30.73	29255136
246	Ubiquitination	FTCGSPTAAGKKTEK CCCCCCCCCCCCCCC	20.01	-
247	Phosphorylation	TCGSPTAAGKKTEKE CCCCCCCCCCCCCCH	32.58	1995645
249	2-Hydroxyisobutyrylation	GSPTAAGKKTEKEES CCCCCCCCCCCCHHH	52.36	-
249	Acetylation	GSPTAAGKKTEKEES CCCCCCCCCCCCHHH	52.36	26051181
250	Acetylation	SPTAAGKKTEKEEST CCCCCCCCCCCHHHH	62.08	26051181
251	Phosphorylation	PTAAGKKTEKEESTE CCCCCCCCCCHHHHH	56.63	26074081
253	2-Hydroxyisobutyrylation	AAGKKTEKEESTEVL CCCCCCCCHHHHHHH	72.51	-
254	Ubiquitination	AGKKTEKEESTEVLK CCCCCCCHHHHHHHH	51.45	-
256	Phosphorylation	KKTEKEESTEVLKAQ CCCCCHHHHHHHHHH	30.84	21815630
257	Phosphorylation	KTEKEESTEVLKAQS CCCCHHHHHHHHHHH	33.13	21815630
261	Methylation	EESTEVLKAQSAGTV HHHHHHHHHHHCCCC	49.94	-
264	Phosphorylation	TEVLKAQSAGTVRSA HHHHHHHHCCCCCCC	33.26	20068231
265	Phosphorylation	EVLKAQSAGTVRSAA HHHHHHHCCCCCCCC	12.95	-
266 (in isoform 5)	Phosphorylation	-	29.95	23090842
267	Phosphorylation	LKAQSAGTVRSAAPP HHHHHCCCCCCCCCH	16.68	26853621
270	Phosphorylation	QSAGTVRSAAPPQEK HHCCCCCCCCCHHHH	25.17	25159151
277	2-Hydroxyisobutyrylation	SAAPPQEKKRKVEKD CCCCHHHHCCCCCCC	53.26	-
278	Phosphorylation	AAPPQEKKRKVEKDT CCCHHHHCCCCCCCC	57.61	-
279	Phosphorylation	APPQEKKRKVEKDTM CCHHHHCCCCCCCCC	60.36	18669648
279 (in isoform 9)	Phosphorylation	-	60.36	27251275
282	Phosphorylation	QEKKRKVEKDTMSDQ HHHCCCCCCCCCCHH	48.53	18669648
284 (in isoform 5)	Phosphorylation	-	33.85	28450419
285	Phosphorylation	KRKVEKDTMSDQALE CCCCCCCCCCHHHHH	29.64	23403867
285 (in isoform 5)	Phosphorylation	-	29.64	28450419
285 (in isoform 9)	Phosphorylation	-	29.64	24719451
286	Sulfoxidation	RKVEKDTMSDQALEA CCCCCCCCCHHHHHH	6.28	21406390
287	Phosphorylation	KVEKDTMSDQALEAL CCCCCCCCHHHHHHH	28.76	23403867
288 (in isoform 5)	Phosphorylation	-	27.27	28450419
291 (in isoform 5)	Phosphorylation	-	3.08	23927012
293 (in isoform 5)	Phosphorylation	-	13.99	28450419
295	Phosphorylation	DQALEALSASLGTRQ HHHHHHHHHHCCCCC	23.90	23403867
297	Phosphorylation	ALEALSASLGTRQAE HHHHHHHHCCCCCCC	24.77	20201521
300	Phosphorylation	ALSASLGTRQAEPEL HHHHHCCCCCCCCCC	25.54	23403867
303	Methylation	ASLGTRQAEPELDLR HHCCCCCCCCCCCHH	30.90	-
303	Ubiquitination	ASLGTRQAEPELDLR HHCCCCCCCCCCCHH	30.90	-
311	Phosphorylation	EPELDLRSIKEVDEA CCCCCHHHHHHHHHH	45.36	25159151
313	2-Hydroxyisobutyrylation	ELDLRSIKEVDEAKA CCCHHHHHHHHHHHH	53.59	-
313	Acetylation	ELDLRSIKEVDEAKA CCCHHHHHHHHHHHH	53.59	12433401
325	Ubiquitination	AKAKEEKLEKCGEDD HHHHHHHHHHCCCCC	9.58	-
329	Phosphorylation	EEKLEKCGEDDETIP HHHHHHCCCCCCCCC	53.05	-
329 (in isoform 9)	Phosphorylation	-	53.05	27251275
337	Phosphorylation	EDDETIPSEYRLKPA CCCCCCCHHHCCCCC	43.33	26356563
337 (in isoform 9)	Phosphorylation	-	43.33	24719451
339	Phosphorylation	DETIPSEYRLKPATD CCCCCHHHCCCCCCC	26.11	26356563
342	Phosphorylation	IPSEYRLKPATDKDG CCHHHCCCCCCCCCC	24.49	-
353	Phosphorylation	DKDGKPLLPEPEEKP CCCCCCCCCCCCCCC	6.48	-
353 (in isoform 9)	Phosphorylation	-	6.48	24719451
364	Phosphorylation	EEKPKPRSESELIDE CCCCCCCCHHHHHHH	54.74	25159151
366	Phosphorylation	KPKPRSESELIDELS CCCCCCHHHHHHHHH	38.66	25159151
373	Phosphorylation	SELIDELSEDFDRSE HHHHHHHHHHCCHHH	32.83	30266825
379	Phosphorylation	LSEDFDRSECKEKPS HHHHCCHHHHHCCCC	49.55	25159151
381	Glutathionylation	EDFDRSECKEKPSKP HHCCHHHHHCCCCCC	7.91	22555962
386	Phosphorylation	SECKEKPSKPTEKTE HHHHCCCCCCCCCCH	63.44	26657352
389	Phosphorylation	KEKPSKPTEKTEESK HCCCCCCCCCCHHHH	54.58	26657352
392	Phosphorylation	PSKPTEKTEESKAAA CCCCCCCCHHHHCCC	38.46	26657352
403	Ubiquitination	KAAAPAPVSEAVCRT HCCCCCCCCHHHHHH	9.80	-
404	O-linked_Glycosylation	AAAPAPVSEAVCRTS CCCCCCCCHHHHHHC	20.84	23301498
404	Phosphorylation	AAAPAPVSEAVCRTS CCCCCCCCHHHHHHC	20.84	28555341
406	Phosphorylation	APAPVSEAVCRTSMC CCCCCCHHHHHHCCC	9.75	18669648
406 (in isoform 9)	Phosphorylation	-	9.75	24719451
408	S-nitrosocysteine	APVSEAVCRTSMCSI CCCCHHHHHHCCCCC	5.18	-
408	Glutathionylation	APVSEAVCRTSMCSI CCCCHHHHHHCCCCC	5.18	22555962
408	Phosphorylation	APVSEAVCRTSMCSI CCCCHHHHHHCCCCC	5.18	18669648
408	S-nitrosylation	APVSEAVCRTSMCSI CCCCHHHHHHCCCCC	5.18	22178444
408 (in isoform 9)	Phosphorylation	-	5.18	24719451
410	Phosphorylation	VSEAVCRTSMCSIQS CCHHHHHHCCCCCCC	18.71	25159151
411	Phosphorylation	SEAVCRTSMCSIQSA CHHHHHHCCCCCCCC	9.84	25159151
412	Sulfoxidation	EAVCRTSMCSIQSAP HHHHHHCCCCCCCCC	1.74	21406390
413	S-nitrosylation	AVCRTSMCSIQSAPP HHHHHCCCCCCCCCC	3.04	2212679
414	Phosphorylation	VCRTSMCSIQSAPPE HHHHCCCCCCCCCCC	18.28	25159151
415	Phosphorylation	CRTSMCSIQSAPPEP HHHCCCCCCCCCCCC	2.98	18669648
415 (in isoform 9)	Phosphorylation	-	2.98	24719451
417	Phosphorylation	TSMCSIQSAPPEPAT HCCCCCCCCCCCCCC	40.86	20068231
421	Phosphorylation	SIQSAPPEPATLKGT CCCCCCCCCCCCCCC	46.65	18669648
421 (in isoform 9)	Phosphorylation	-	46.65	27251275
424	Phosphorylation	SAPPEPATLKGTVPD CCCCCCCCCCCCCCH	39.45	20068231
431	Phosphorylation	TLKGTVPDDAVEALA CCCCCCCHHHHHHHH	52.76	-
440	Phosphorylation	AVEALADSLGKKEAD HHHHHHHHHCCCCCC	33.15	29255136
443	Acetylation	ALADSLGKKEADPED HHHHHHCCCCCCCCC	53.38	26051181
452	Acetylation	EADPEDGKPVMDKVK CCCCCCCCCCHHHHH	46.92	26051181
452	Phosphorylation	EADPEDGKPVMDKVK CCCCCCCCCCHHHHH	46.92	18669648
453	Phosphorylation	ADPEDGKPVMDKVKE CCCCCCCCCHHHHHH	31.99	18669648
455	Sulfoxidation	PEDGKPVMDKVKEKA CCCCCCCHHHHHHHH	5.83	30846556
456	Phosphorylation	EDGKPVMDKVKEKAK CCCCCCHHHHHHHHH	53.48	-
456 (in isoform 9)	Phosphorylation	-	53.48	24719451
457	2-Hydroxyisobutyrylation	DGKPVMDKVKEKAKE CCCCCHHHHHHHHHH	36.79	-
457	Acetylation	DGKPVMDKVKEKAKE CCCCCHHHHHHHHHH	36.79	26051181
459	Phosphorylation	KPVMDKVKEKAKEED CCCHHHHHHHHHHHH	60.27	-
473	2-Hydroxyisobutyrylation	DREKLGEKEETIPPD HHHHHCCCCCCCCCC	60.08	-
480 (in isoform 3)	Ubiquitination	-	35.51	21906983
481	Phosphorylation	EETIPPDYRLEEVKD CCCCCCCCCHHHCCC	23.91	25394399
482	Phosphorylation	ETIPPDYRLEEVKDK CCCCCCCCHHHCCCC	42.90	-
482 (in isoform 9)	Phosphorylation	-	42.90	24719451
485	Ubiquitination	PPDYRLEEVKDKDGK CCCCCHHHCCCCCCC	60.50	-
493 (in isoform 4)	Ubiquitination	-	44.66	21906983
499	Phosphorylation	KPLLPKESKEQLPPM CCCCCHHHHHHCCCC	47.85	25159151
500	Acetylation	PLLPKESKEQLPPMS CCCCHHHHHHCCCCC	51.04	20167786
506	Sulfoxidation	SKEQLPPMSEDFLLD HHHHCCCCCHHHHHH	6.91	30846556
507	Phosphorylation	KEQLPPMSEDFLLDA HHHCCCCCHHHHHHH	39.53	22199227
516	Phosphorylation	DFLLDALSEDFSGPQ HHHHHHHCCCCCCCC	36.52	22199227
520	Phosphorylation	DALSEDFSGPQNASS HHHCCCCCCCCCHHH	62.98	22199227
521 (in isoform 2)	Ubiquitination	-	28.01	21906983
523	Phosphorylation	SEDFSGPQNASSLKF CCCCCCCCCHHHCCH	62.74	-
526	Phosphorylation	FSGPQNASSLKFEDA CCCCCCHHHCCHHHH	43.69	22199227
527	Phosphorylation	SGPQNASSLKFEDAK CCCCCHHHCCHHHHH	32.92	29496963
529	Acetylation	PQNASSLKFEDAKLA CCCHHHCCHHHHHHH	48.97	20167786
534	Ubiquitination	SLKFEDAKLAAAISE HCCHHHHHHHHHHHH	51.48	2190698
534 (in isoform 1)	Ubiquitination	-	51.48	21906983
540	Phosphorylation	AKLAAAISEVVSQTP HHHHHHHHHHHHCCC	21.44	20068231
541	Phosphorylation	KLAAAISEVVSQTPA HHHHHHHHHHHCCCC	40.60	-
541 (in isoform 9)	Phosphorylation	-	40.60	27251275
542	Acetylation	LAAAISEVVSQTPAS HHHHHHHHHHCCCCC	3.92	-
542	Ubiquitination	LAAAISEVVSQTPAS HHHHHHHHHHCCCCC	3.92	-
544	Phosphorylation	AAISEVVSQTPASTT HHHHHHHHCCCCCCC	33.25	30576142
546	Phosphorylation	ISEVVSQTPASTTQA HHHHHHCCCCCCCCC	17.20	30576142
549	Phosphorylation	VVSQTPASTTQAGAP HHHCCCCCCCCCCCC	32.94	26657352
550	Phosphorylation	VSQTPASTTQAGAPP HHCCCCCCCCCCCCC	25.65	23090842
551	Phosphorylation	SQTPASTTQAGAPPR HCCCCCCCCCCCCCC	17.58	30576142
558	Phosphorylation	TQAGAPPRDTSQSDK CCCCCCCCCCCCCCH	59.80	18669648
558 (in isoform 9)	Phosphorylation	-	59.80	24719451
560	Phosphorylation	AGAPPRDTSQSDKDL CCCCCCCCCCCCHHH	29.91	25159151
561	Phosphorylation	GAPPRDTSQSDKDLD CCCCCCCCCCCHHHH	31.79	23927012
563	Phosphorylation	PPRDTSQSDKDLDDA CCCCCCCCCHHHHHH	46.53	25159151
568	Phosphorylation	SQSDKDLDDALDKLS CCCCHHHHHHHHHHH	49.63	-
569	Phosphorylation	QSDKDLDDALDKLSD CCCHHHHHHHHHHHH	57.22	-
569 (in isoform 9)	Phosphorylation	-	57.22	27251275
571	Acetylation	DKDLDDALDKLSDSL CHHHHHHHHHHHHHH	8.42	-
573	Acetylation	DLDDALDKLSDSLGQ HHHHHHHHHHHHHCC	51.22	23236377
575	Phosphorylation	DDALDKLSDSLGQRQ HHHHHHHHHHHCCCC	30.78	25463755
576	Ubiquitination	DALDKLSDSLGQRQP HHHHHHHHHHCCCCC	59.32	-
577	Phosphorylation	ALDKLSDSLGQRQPD HHHHHHHHHCCCCCC	30.90	29255136
580	Ubiquitination	KLSDSLGQRQPDPDE HHHHHHCCCCCCCCC	46.24	21906983
587	Acetylation	QRQPDPDENKPMEDK CCCCCCCCCCCCHHH	72.97	19608861
588	Phosphorylation	RQPDPDENKPMEDKV CCCCCCCCCCCHHHH	62.20	-
591	Sulfoxidation	DPDENKPMEDKVKEK CCCCCCCCHHHHHHH	12.73	30846556
591 (in isoform 9)	Phosphorylation	-	12.73	24719451
592 (in isoform 9)	Phosphorylation	-	64.31	27251275
594	Acetylation	ENKPMEDKVKEKAKA CCCCCHHHHHHHHHH	41.73	-
603	Phosphorylation	KEKAKAEHRDKLGER HHHHHHHHHHHHCCC	48.36	18669648
603 (in isoform 9)	Phosphorylation	-	48.36	24719451
605	Phosphorylation	KAKAEHRDKLGERDD HHHHHHHHHHCCCCC	53.06	18669648
607	Acetylation	KAEHRDKLGERDDTI HHHHHHHHCCCCCCC	11.46	19608861
613	Phosphorylation	KLGERDDTIPPEYRH HHCCCCCCCCHHHHH	39.41	29214152
615	Acetylation	GERDDTIPPEYRHLL CCCCCCCCHHHHHHH	20.12	-
615	Ubiquitination	GERDDTIPPEYRHLL CCCCCCCCHHHHHHH	20.12	-
617	Phosphorylation	RDDTIPPEYRHLLDD CCCCCCHHHHHHHCC	50.25	-
617 (in isoform 9)	Phosphorylation	-	50.25	24719451
618	Phosphorylation	DDTIPPEYRHLLDDN CCCCCHHHHHHHCCC	14.76	23917254
619	Acetylation	DTIPPEYRHLLDDNG CCCCHHHHHHHCCCC	16.59	-
619	Phosphorylation	DTIPPEYRHLLDDNG CCCCHHHHHHHCCCC	16.59	18669648
619 (in isoform 9)	Phosphorylation	-	16.59	24719451
629	Acetylation	LDDNGQDKPVKPPTK HCCCCCCCCCCCCCC	43.77	23954790
635	Phosphorylation	DKPVKPPTKKSEDSK CCCCCCCCCCCCCCC	60.68	28555341
638	Phosphorylation	VKPPTKKSEDSKKPA CCCCCCCCCCCCCCC	48.47	20166139
641	Phosphorylation	PTKKSEDSKKPADDQ CCCCCCCCCCCCCCC	38.23	20166139
648	Ubiquitination	SKKPADDQDPIDALS CCCCCCCCCHHHHHC	59.95	-
655	Phosphorylation	QDPIDALSGDLDSCP CCHHHHHCCCCCCCC	31.79	25159151
660	Phosphorylation	ALSGDLDSCPSTTET HHCCCCCCCCCCCCC	34.91	26657352
661	S-nitrosocysteine	LSGDLDSCPSTTETS HCCCCCCCCCCCCCC	2.85	-
661	S-nitrosylation	LSGDLDSCPSTTETS HCCCCCCCCCCCCCC	2.85	22178444
663	Phosphorylation	GDLDSCPSTTETSQN CCCCCCCCCCCCCCH	52.90	25693802
664	Phosphorylation	DLDSCPSTTETSQNT CCCCCCCCCCCCCHH	17.50	26657352
665	Phosphorylation	LDSCPSTTETSQNTA CCCCCCCCCCCCHHH	41.42	30242111
667	Phosphorylation	SCPSTTETSQNTAKD CCCCCCCCCCHHHHH	33.26	22798277
668	Phosphorylation	CPSTTETSQNTAKDK CCCCCCCCCHHHHHH	18.39	30242111
671	Acetylation	TTETSQNTAKDKCKK CCCCCCHHHHHHHHH	27.70	19608861
671	Phosphorylation	TTETSQNTAKDKCKK CCCCCCHHHHHHHHH	27.70	21712546
671	Ubiquitination	TTETSQNTAKDKCKK CCCCCCHHHHHHHHH	27.70	-
673	Acetylation	ETSQNTAKDKCKKAA CCCCHHHHHHHHHHH	56.53	26051181
674	Ubiquitination	TSQNTAKDKCKKAAS CCCHHHHHHHHHHHH	60.15	-
677	Acetylation	NTAKDKCKKAASSSK HHHHHHHHHHHHCCC	51.71	-
678	Ubiquitination	TAKDKCKKAASSSKA HHHHHHHHHHHCCCC	59.67	-
682	Phosphorylation	KCKKAASSSKAPKNG HHHHHHHCCCCCCCC	31.00	21601212
684	Acetylation	KKAASSSKAPKNGGK HHHHHCCCCCCCCCC	71.32	30587407
687	Acetylation	ASSSKAPKNGGKAKD HHCCCCCCCCCCCCC	73.43	30587413
691	Acetylation	KAPKNGGKAKDSAKT CCCCCCCCCCCCCCC	54.37	30587419
693	Acetylation	PKNGGKAKDSAKTTE CCCCCCCCCCCCCCC	56.56	-
697	Phosphorylation	GKAKDSAKTTEETSK CCCCCCCCCCCCCCC	60.99	-
702	Phosphorylation	SAKTTEETSKPKDD- CCCCCCCCCCCCCC-	35.50	-
706	Phosphorylation	TEETSKPKDD----- CCCCCCCCCC-----	77.57	-
710	Phosphorylation	SKPKDD--------- CCCCCC---------		-
713	Phosphorylation	KDD------------ CCC------------		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
655	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ICAL_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ICAL_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CAN1_HUMAN	CAPN1	physical	12684003
PSIP1_HUMAN	PSIP1	physical	22939629
PSA5_HUMAN	PSMA5	physical	22863883
KCRB_HUMAN	CKB	physical	26344197
CRK_HUMAN	CRK	physical	26344197
LZIC_HUMAN	LZIC	physical	26344197
PPME1_HUMAN	PPME1	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
616295	Peeling skin with leukonychia, acral punctate keratoses, cheilitis, and knuckle pads (PLACK)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ICAL_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-629, AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243; SER-364; SER-366AND SER-373, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Global proteomic profiling of phosphopeptides using electron transferdissociation tandem mass spectrometry."; Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.; Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, AND MASSSPECTROMETRY.	17287340 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243, AND MASSSPECTROMETRY.	17081983 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243, AND MASSSPECTROMETRY.	15302935 [Abstract]