dbPTM

HNF4A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HNF4A_MOUSE
UniProt AC	P49698
Protein Name	Hepatocyte nuclear factor 4-alpha
Gene Name	Hnf4a
Organism	Mus musculus (Mouse).
Sequence Length	474
Subcellular Localization	Nucleus.
Protein Description	Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine..
Protein Sequence	MRLSKTLAGMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHTHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
139	Phosphorylation	NERDRISTRRSSYED CHHHHHHHCHHHCCC	27.94	-
142	Phosphorylation	DRISTRRSSYEDSSL HHHHHCHHHCCCCCC	33.70	22817900
143	Phosphorylation	RISTRRSSYEDSSLP HHHHCHHHCCCCCCH	30.63	22817900
144	Phosphorylation	ISTRRSSYEDSSLPS HHHCHHHCCCCCCHH	25.63	-
147	Phosphorylation	RRSSYEDSSLPSINA CHHHCCCCCCHHHHH	23.10	23984901
148	Phosphorylation	RSSYEDSSLPSINAL HHHCCCCCCHHHHHH	57.53	23984901
151	Phosphorylation	YEDSSLPSINALLQA CCCCCCHHHHHHHHH	33.49	23984901
162	Phosphorylation	LLQAEVLSQQITSPI HHHHHHHHHHCCCCC	25.80	23984901
166	Phosphorylation	EVLSQQITSPISGIN HHHHHHCCCCCCCCC	24.90	23984901
167	Phosphorylation	VLSQQITSPISGING HHHHHCCCCCCCCCC	23.12	22817900
170	Phosphorylation	QQITSPISGINGDIR HHCCCCCCCCCCCHH	36.68	23984901
180	Ubiquitination	NGDIRAKKIANITDV CCCHHHHHHHCHHHH	46.17	22790023
188	S-palmitoylation	IANITDVCESMKEQL HHCHHHHHHHHHHHH	3.40	28526873
234	Ubiquitination	HLLLGATKRSMVFKD HHHHCCHHHHHHHHC	41.77	22790023
300	Ubiquitination	IFFDPDAKGLSDPGK EEECCCCCCCCCHHH	68.70	22790023
313	Phosphorylation	GKIKRLRSQVQVSLE HHHHHHHHHEEEEHH	39.02	-
429	Phosphorylation	RPRGQAATPETPQPS CCCCCCCCCCCCCCC	25.36	25521595
432	Phosphorylation	GQAATPETPQPSPPS CCCCCCCCCCCCCCC	28.55	25521595
436	Phosphorylation	TPETPQPSPPSGSGS CCCCCCCCCCCCCCC	44.17	25521595
439	Phosphorylation	TPQPSPPSGSGSESY CCCCCCCCCCCCCCE	49.31	19060867
441	Phosphorylation	QPSPPSGSGSESYKL CCCCCCCCCCCCEEE	43.41	19060867
443	Phosphorylation	SPPSGSGSESYKLLP CCCCCCCCCCEEECC	25.68	25521595
445	Phosphorylation	PSGSGSESYKLLPGA CCCCCCCCEEECCCC	29.30	23984901
446	Phosphorylation	SGSGSESYKLLPGAI CCCCCCCEEECCCCE	11.12	23984901
458	Acetylation	GAITTIVKPPSAIPQ CCEEEEECCCCCCCC	47.57	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
143	S	Phosphorylation	Kinase	PKACA	P17612	PSP
143	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
313	S	Phosphorylation	Kinase	AMPK	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
313	S	Phosphorylation	-
Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA (By similarity).
458	K	Acetylation	-
Acetylation at Lys-458 lowers transcriptional activation by about two-fold.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HNF4A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
TF7L2_MOUSE	Tcf7l2	physical	19805521
NR0B1_MOUSE	Nr0b1	physical	19651776

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HNF4A_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-429 AND THR-432, ANDMASS SPECTROMETRY.	17242355 [Abstract]

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