dbPTM

HCLS1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HCLS1_MOUSE
UniProt AC	P49710
Protein Name	Hematopoietic lineage cell-specific protein
Gene Name	Hcls1
Organism	Mus musculus (Mouse).
Sequence Length	486
Subcellular Localization	Mitochondrion.
Protein Description	Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression (By similarity)..
Protein Sequence	MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKKKELESGPKASHGYGGRFGVERDRMDKSAVGHEYVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDYKGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSREVQQPSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSPLQNHLEDNEEPPALPPRTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVGELDRQDEDAEGDYEDVLEPEDTPSLSYQAGPSAGAGGAGISAIALYDYQGEGSDELSFDPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
41	Acetylation	KEQRWGAKTIEGSGR HHHHHCCEECCCCCC	46.20	-
62	Phosphorylation	HQLRNKVSEEHDILK HHHHHHHHHHHHHHH	38.59	27600695
97	Phosphorylation	ERDRMDKSAVGHEYV CCCCCCHHHCCCEEE	24.75	25367039
103	Phosphorylation	KSAVGHEYVADVEKH HHHCCCEEEEHHHHH	8.68	25159016
123	Acetylation	AARGFGGKYGVERDR HHHCCCCCCCCCCCC	38.93	-
124	Phosphorylation	ARGFGGKYGVERDRA HHCCCCCCCCCCCCC	29.67	25367039
140	Phosphorylation	KSAVGFDYKGEVEKH HHCCCCCCCCCHHHH	20.73	25159016
149	Phosphorylation	GEVEKHASQKDYSHG CCHHHHCCCCCCCCC	37.17	25266776
153	Phosphorylation	KHASQKDYSHGFGGR HHCCCCCCCCCCCCC	15.03	25159016
154	Phosphorylation	HASQKDYSHGFGGRY HCCCCCCCCCCCCCC	27.54	25367039
175	Phosphorylation	RDKAALGYDYKGETE HHHHHCCCCCCCCCC	19.56	25367039
177	Phosphorylation	KAALGYDYKGETEKH HHHCCCCCCCCCCCC	16.65	-
186	Phosphorylation	GETEKHESQRDYAKG CCCCCCHHHHHHHHC	30.18	25367039
190	Phosphorylation	KHESQRDYAKGFGGQ CCHHHHHHHHCCCCC	16.64	25367039
192	Acetylation	ESQRDYAKGFGGQYG HHHHHHHHCCCCCCC	48.85	-
198	Phosphorylation	AKGFGGQYGIQKDRV HHCCCCCCCCCCCCC	21.33	25159016
220	Phosphorylation	NEMEAPTTAYKKTTP CCCCCCCCCCCCCCC	27.25	25367039
222	Phosphorylation	MEAPTTAYKKTTPIE CCCCCCCCCCCCCHH	15.78	22817900
225	Phosphorylation	PTTAYKKTTPIEAAS CCCCCCCCCCHHHHH	33.38	29176673
226	Phosphorylation	TTAYKKTTPIEAASS CCCCCCCCCHHHHHH	31.02	29176673
232	Phosphorylation	TTPIEAASSGARGLK CCCHHHHHHCCHHHH	36.03	29176673
233	Phosphorylation	TPIEAASSGARGLKA CCHHHHHHCCHHHHH	31.57	29176673
241	Acetylation	GARGLKAKFESLAEE CCHHHHHHHHHHHHH	48.34	-
244	Phosphorylation	GLKAKFESLAEEKRK HHHHHHHHHHHHHHH	35.69	28059163
275	Phosphorylation	RKAVVKMSREVQQPS HHHHHHHHHHHCCCC	21.41	27600695
282	Phosphorylation	SREVQQPSMPVEEPA HHHHCCCCCCCCCCC	31.23	24719451
299	Phosphorylation	AQLPKKISSEVWPPA CCCCCCCCCCCCCCH	29.35	25266776
300	Phosphorylation	QLPKKISSEVWPPAE CCCCCCCCCCCCCHH	39.37	27180971
319	Phosphorylation	PESQPVRSRREYPVP CCCCCCCCCCCCCCC	35.60	25367039
323	Phosphorylation	PVRSRREYPVPSLPT CCCCCCCCCCCCCCC	14.02	28833060
327	Phosphorylation	RREYPVPSLPTRQSP CCCCCCCCCCCCCCC	47.41	28833060
330	Phosphorylation	YPVPSLPTRQSPLQN CCCCCCCCCCCCCHH	46.78	28725479
333	Phosphorylation	PSLPTRQSPLQNHLE CCCCCCCCCCHHHCC	25.04	27742792
364	Phosphorylation	QVVEEPVYEAAPELE EEEEEEHHHCCCCCC	15.57	-
384	Phosphorylation	DYEPEPETEPDYEDV CCCCCCCCCCCCCCC	64.63	-
388	Phosphorylation	EPETEPDYEDVGELD CCCCCCCCCCCCCCC	25.41	19917685
405	Phosphorylation	DEDAEGDYEDVLEPE CCCCCCCHHHCCCCC	25.53	19917685
471	Glutathionylation	EGWWRGQCRGHFGLF CCCCCCCCCCCCCEE	6.65	24333276

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
222	Y	Phosphorylation	Kinase	FGR	P14234	Uniprot
388	Y	Phosphorylation	Kinase	FES	P16879	Uniprot
388	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot
405	Y	Phosphorylation	Kinase	FES	P16879	Uniprot
405	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of HCLS1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HCLS1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
NFE2_MOUSE	Nfe2	physical	20211142
H1BP3_MOUSE	Hs1bp3	physical	10590261

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HCLS1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative time-resolved phosphoproteomic analysis of mast cellsignaling."; Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,Kawakami T., Salomon A.R.; J. Immunol. 179:5864-5876(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-140, AND MASSSPECTROMETRY.	17947660 [Abstract]