GRB14_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GRB14_HUMAN
• UniProt AC: Q14449
• Protein Name: Growth factor receptor-bound protein 14
• Gene Name: GRB14
• Organism: Homo sapiens (Human).
• Sequence Length: 540
• Subcellular Localization: Cytoplasm . Endosome membrane ; Peripheral membrane protein . Upon insulin stimulation, translocates to the plasma membrane.
• Protein Description: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal..
• Protein Sequence: MTTSLQDGQSAASRAAARDSPLAAQVCGAAQGRGDAHDLAPAPWLHARALLPLPDGTRGCAADRRKKKDLDVPEMPSIPNPFPELCCSPFTSVLSADLFPKANSRKKQVIKVYSEDETSRALDVPSDITARDVCQLLILKNHYIDDHSWTLFEHLPHIGVERTIEDHELVIEVLSNWGIEEENKLYFRKNYAKYEFFKNPMYFFPEHMVSFATETNGEISPTQILQMFLSSSTYPEIHGFLHAKEQGKKSWKKIYFFLRRSGLYFSTKGTSKEPRHLQFFSEFGNSDIYVSLAGKKKHGAPTNYGFCFKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKYGMQLYQNYMHPYQGRSGCSSQSISPMRSISENSLVAMDFSGQKSRVIENPTEALSVAVEEGLAWRKKGCLRLGTHGSPTASSQSSATNMAIHRSQPWFHHKISRDEAQRLIIQQGLVDGVFLVRDSQSNPKTFVLSMSHGQKIKHFQIIPVEDDGEMFHTLDDGHTRFTDLIQLVEFYQLNKGVLPCKLKHYCARIAL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MTTSLQDGQ ------CCCCCHHHH	25.70	19413330
3	Phosphorylation	-----MTTSLQDGQS -----CCCCCHHHHH	26.58	22210691
9 (in isoform 2)	Phosphorylation	-	37.88	25849741
13	Phosphorylation	QDGQSAASRAAARDS HHHHHHHHHHHHCCC	23.36	22210691
14 (in isoform 2)	Phosphorylation	-	24.42	24275569
20	Phosphorylation	SRAAARDSPLAAQVC HHHHHCCCHHHHHHH	19.09	22210691
113	Phosphorylation	KKQVIKVYSEDETSR CCEEEEEECCCCCCC	11.01	22817900
358	Phosphorylation	MHPYQGRSGCSSQSI CCCCCCCCCCCCCCC	51.48	-
362	Phosphorylation	QGRSGCSSQSISPMR CCCCCCCCCCCCCCC	31.83	-
366	Phosphorylation	GCSSQSISPMRSISE CCCCCCCCCCCCCCC	20.21	28857561
370	Phosphorylation	QSISPMRSISENSLV CCCCCCCCCCCCCEE	24.98	20166139
372	Phosphorylation	ISPMRSISENSLVAM CCCCCCCCCCCEEEE	31.78	25554490
375	Phosphorylation	MRSISENSLVAMDFS CCCCCCCCEEEECCC	21.85	23403867
416	Phosphorylation	KGCLRLGTHGSPTAS HCCEEECCCCCCCCC	27.88	29978859
419	Phosphorylation	LRLGTHGSPTASSQS EEECCCCCCCCCCCC	16.09	25849741
421	Phosphorylation	LGTHGSPTASSQSSA ECCCCCCCCCCCCHH	40.65	29978859
423	Phosphorylation	THGSPTASSQSSATN CCCCCCCCCCCHHHH	31.24	29978859
424	Phosphorylation	HGSPTASSQSSATNM CCCCCCCCCCHHHHH	31.45	29978859
426	Phosphorylation	SPTASSQSSATNMAI CCCCCCCCHHHHHHH	24.72	23312004
427	Phosphorylation	PTASSQSSATNMAIH CCCCCCCHHHHHHHH	31.32	29978859

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
358	S	Phosphorylation	Kinase	CK2A1	P68400	PSP
358	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
358	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
362	S	Phosphorylation	Kinase	CK2A1	P68400	PSP
362	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
362	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
366	S	Phosphorylation	Kinase	CK2A1	P68400	PSP
366	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
366	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
370	S	Phosphorylation	Kinase	CK2A1	P68400	PSP
370	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
419	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
419	S	Phosphorylation	Kinase	GSK3B	P49841	PSP
423	S	Phosphorylation	Kinase	GSK3A	P49840	PSP
423	S	Phosphorylation	Kinase	GSK3B	P49841	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GRB14_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GRB14_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KPCZ_HUMAN	PRKCZ	physical	12242277
EGFR_HUMAN	EGFR	physical	8647858
PGFRB_HUMAN	PDGFRB	physical	8647858
TNKS2_HUMAN	TNKS2	physical	11278563
FGFR1_HUMAN	FGFR1	physical	10713090
IGF1R_HUMAN	IGF1R	physical	21988832
CHFR_HUMAN	CHFR	physical	25578860
CHFR_XENLA	chfr	physical	25578860
STK26_HUMAN	STK26	physical	25814554

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.