FBXW8_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: FBXW8_MOUSE
• UniProt AC: Q8BIA4
• Protein Name: F-box/WD repeat-containing protein 8
• Gene Name: Fbxw8
• Organism: Mus musculus (Mouse).
• Sequence Length: 598
• Subcellular Localization: Cytoplasm, perinuclear region . Golgi apparatus . Colocalizes with CUL7 at the Golgi apparatus in neurons.
• Protein Description: Substrate-recognition component of a Cul7-RING ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (By similarity)..
• Protein Sequence: MDDHNLEEFRRHWQEELAQSQALRRRRRLEAGERRSPRRPEAGARGEPASGYLGLAQGLLEGAGRPPAPRPGRGGDRKDTSSRSRSPPDRDATEPEPLVDQLIRDLNELDDVPFFDVRLPYELAINIFQYLNRRELGLCAQVSKTWKVIAEDEVLWYRLCRQEGHLPHSRFSDYTCWKLILQECLAKEHTLRANWKNRKGAVSELEHVPDAVLCDVRSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESESEEEDPGMQPYVSFVRINSSLAVAAYEDGILNIWDLRTGRFPIFRFEHDARIQALALSQEKPIVATASAFDVVMLYPNEEGHWHVASEFEVQKLVDYLEIVPNTGRYPVAIATAGDLVYLLKADDSARTLHYVYGQPATCLDVSASQVAFGVKSLGWVYEGNKILVYSLEAERCLSKLGNALGDFTCVNIRDSPPNLMVSGNMDRRVRIHDLRSDKIALSLSAHQLGVSAVQMDDWKVVSGGEEGLVSVWDYRMNQKLWEVHSRHPVRYLSFNSHSLITANVPYEKVLRNSDLDNFACHRRHRGLIHAYEFAVDQLAFQSPLPVCRLPRDIMAGYSYDLALSFPHDSI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDDHNLEE -------CCCCCHHH	14.25	-
36	Phosphorylation	LEAGERRSPRRPEAG HHHCCCCCCCCCCCC	29.04	26824392
80	Phosphorylation	RGGDRKDTSSRSRSP CCCCCCCCCCCCCCC	31.47	27841257
81	Phosphorylation	GGDRKDTSSRSRSPP CCCCCCCCCCCCCCC	33.61	27841257
82	Phosphorylation	GDRKDTSSRSRSPPD CCCCCCCCCCCCCCC	36.05	27841257
84	Phosphorylation	RKDTSSRSRSPPDRD CCCCCCCCCCCCCCC	38.77	26824392
86	Phosphorylation	DTSSRSRSPPDRDAT CCCCCCCCCCCCCCC	41.87	26824392
93	Phosphorylation	SPPDRDATEPEPLVD CCCCCCCCCCCHHHH	57.26	27742792
104	Dimethylation	PLVDQLIRDLNELDD HHHHHHHHHHHHCCC	51.46	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
86	S	Phosphorylation	Kinase	MTOR	P42345	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of FBXW8_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of FBXW8_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CUL1_MOUSE	Cul1	physical	16880526
CUL7_MOUSE	Cul7	physical	16880526
SKP1_MOUSE	Skp1a	physical	16880526
RBX1_MOUSE	Rbx1	physical	16880526

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, AND MASSSPECTROMETRY.
PubMed: 17242355