dbPTM

ETS1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ETS1_MOUSE
UniProt AC	P27577
Protein Name	Protein C-ets-1
Gene Name	Ets1
Organism	Mus musculus (Mouse).
Sequence Length	440
Subcellular Localization	Cytoplasm . Nucleus . Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Protein Description	Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (By similarity)..
Protein Sequence	MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEMMSQALKATFSGFTKEQQRLGIPKDPRQWTETHVRDWVMWAVNEFSLKGVDFQKFCMSGAALCALGKECFLELAPDFVGDILWEHLEILQKEDVKPYQVNGANPTYPESCYTSDYFISYGIEHAQCVPPSEFSEPSFITESYQTLHPISSEELLSLKYENDYPSVILQDPLQTDTLQTDYFAIKQEVLTPDNMCLGRASRGKLGGQDSFESVESYDSCDRLTQSWSSQSSFNSLQRVPSYDSFDYEDYPAALPNHKPKGTFKDYVRDRADLNKDKPVIPAAALAGYTGSGPIQLWQFLLELLTDKSCQSFISWTGDGWEFKLSDPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQSLLGYTPEELHAMLDVKPDAD

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MKAAVDLKP ------CCCCCCCCC	45.08	22826441
8	Acetylation	MKAAVDLKPTLTIIK CCCCCCCCCEEEEEE	31.34	-
15	Acetylation	KPTLTIIKTEKVDLE CCEEEEEEECCCCCC	46.94	23236377
15	Sumoylation	KPTLTIIKTEKVDLE CCEEEEEEECCCCCC	46.94	-
15	Sumoylation	KPTLTIIKTEKVDLE CCEEEEEEECCCCCC	46.94	-
26	Phosphorylation	VDLELFPSPDMECAD CCCCCCCCCCCCCCC	26.63	25338131
38	Phosphorylation	CADVPLLTPSSKEMM CCCCCCCCCCHHHHH	28.89	9770451
41	Phosphorylation	VPLLTPSSKEMMSQA CCCCCCCHHHHHHHH	33.56	20534573
58	Ubiquitination	ATFSGFTKEQQRLGI HHHCCCCHHHHHHCC	51.79	22790023
223	Phosphorylation	TDTLQTDYFAIKQEV CCCCCCCCEEEECCE	9.78	-
227	Sumoylation	QTDYFAIKQEVLTPD CCCCEEEECCEECCC	36.66	16862185
227	Sumoylation	QTDYFAIKQEVLTPD CCCCEEEECCEECCC	36.66	-
245	Acetylation	LGRASRGKLGGQDSF CCCCCCCCCCCCCCC	42.73	23236377
251	Phosphorylation	GKLGGQDSFESVESY CCCCCCCCCCCHHHH	24.03	28833060
254	Phosphorylation	GGQDSFESVESYDSC CCCCCCCCHHHHHCC	29.22	28833060
257	Phosphorylation	DSFESVESYDSCDRL CCCCCHHHHHCCCHH	31.89	-
265	Phosphorylation	YDSCDRLTQSWSSQS HHCCCHHCHHCCCCC	22.81	-
267	Phosphorylation	SCDRLTQSWSSQSSF CCCHHCHHCCCCCCC	24.56	27566939
269	Phosphorylation	DRLTQSWSSQSSFNS CHHCHHCCCCCCCCC	24.60	27566939
270	Phosphorylation	RLTQSWSSQSSFNSL HHCHHCCCCCCCCCC	28.02	-
282	Phosphorylation	NSLQRVPSYDSFDYE CCCCCCCCCCCCCCC	38.42	27742792
283	Phosphorylation	SLQRVPSYDSFDYED CCCCCCCCCCCCCCC	14.99	25619855
285	Phosphorylation	QRVPSYDSFDYEDYP CCCCCCCCCCCCCCC	16.28	25619855
288	Phosphorylation	PSYDSFDYEDYPAAL CCCCCCCCCCCCCCC	14.53	25619855
291	Phosphorylation	DSFDYEDYPAALPNH CCCCCCCCCCCCCCC	5.26	29472430
305	Acetylation	HKPKGTFKDYVRDRA CCCCCCHHHHHHHHH	48.24	-
377	Ubiquitination	EVARRWGKRKNKPKM HHHHHHHHCCCCCCC	53.92	-
379	Ubiquitination	ARRWGKRKNKPKMNY HHHHHHCCCCCCCCH	73.19	-
381	Ubiquitination	RWGKRKNKPKMNYEK HHHHCCCCCCCCHHH	49.39	-
405	Phosphorylation	DKNIIHKTAGKRYVY HCCCEECCCCCHHHH	26.87	-
410	Phosphorylation	HKTAGKRYVYRFVCD ECCCCCHHHHHHHHH	12.81	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
38	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
38	T	Phosphorylation	Kinase	ERK2	P63085	PSP
38	T	Phosphorylation	Kinase	MAPK3	P27361	GPS
38	T	Phosphorylation	Kinase	MAPK-FAMILY	-	GPS
38	T	Phosphorylation	Kinase	MAPK	-	Uniprot
41	S	Phosphorylation	Kinase	ERK2	P63085	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
15	K	Sumoylation	16862185
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.
227	K	Sumoylation	16862185
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ETS1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ETS1_MOUSE	Ets1	physical	20211142
HDAC1_MOUSE	Hdac1	physical	22266280
PRDM1_MOUSE	Prdm1	physical	17977828

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ETS1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Structure of the ets-1 pointed domain and mitogen-activated proteinkinase phosphorylation site."; Slupsky C.M., Gentile L.N., Donaldson L.W., Mackereth C.D.,Seidel J.J., Graves B.J., McIntosh L.P.; Proc. Natl. Acad. Sci. U.S.A. 95:12129-12134(1998). Cited for: STRUCTURE BY NMR OF 29-138, AND PHOSPHORYLATION AT THR-38.	9770451 [Abstract]
Sumoylation
Reference	PubMed
"Regulation of the Ets-1 transcription factor by sumoylation andubiquitinylation."; Ji Z., Degerny C., Vintonenko N., Deheuninck J., Foveau B., Leroy C.,Coll J., Tulasne D., Baert J.-L., Fafeur V.; Oncogene 26:395-406(2007). Cited for: SUMOYLATION AT LYS-15 AND LYS-227, AND UBIQUITINATION.	16862185 [Abstract]

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