dbPTM

EP15R_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EP15R_MOUSE
UniProt AC	Q60902
Protein Name	Epidermal growth factor receptor substrate 15-like 1
Gene Name	Eps15l1
Organism	Mus musculus (Mouse).
Sequence Length	907
Subcellular Localization	Cell membrane Peripheral membrane protein . Nucleus . Membrane, coated pit . Localized to plasma membrane coated pits.
Protein Description	Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2 (By similarity)..
Protein Sequence	MAAPLVPLSQQIPGGNPLYESYYKQVDPAYTGRVGASEAALFLKKSGLSDIILGKIWDLADPEGKGFLDKQGFYVALRLVACAQSGHEVTLSSLSLTMPPPKFHDTSSPLMATQSSAETHWAVRVEEKAKFDGIFESLLPVNGLLSGDKVKPVLMNSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSILPPPLIPPSKRKKTVFAGAVPVLPASPPPKDSLRSTPSHGSVSSLNSTGSLSPKHSVKQPPVAWVVPVADKMRFDEIFLKTDLDLDGYVSGQEVKEIFMHSGLTQNLLAHIWALADTRQTGKLSKEQFALAMYFIQQKVSKGIDPPQVLSPDMVPPSERGTPIPDSSSTLASGEFTGVKELDDISQEIAQLQREKYSLEQDIREKEEAIRQKTSEVQELQNDLDRETSSLQELEAQKQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQTISSLKTQIQSQESDLKSQEDDLNRAKSELNRLQQEETQLEQSIQAGRAQLETILRSLKCTQDDINQARSKLSQLQESHLEAHRSLEQYDQVPDGVSGTSLPDLATLNEGILLAERGGFGAMDDPFKNKALLFSNNSQELHPDPFQAEDPFKSDPFKGADPFKGDPFQSDPFSEQQTAATDPFGGDPFKESDPFHSSSSDDFFKKQTKNDPFTSDPFTKNPSLPSKLDPFESSDPFSSSSISSKGSDPFGTLDPFGSSSFSSAEGFADFSQMSKPPPSGPFSSSLGGTGFSDDPFKSKQDTPALPPKKPAPPRPKPPSGQSTPVSQLGSSDFPESPDPFQPLGADSGDPFQNKKGFGDPFSGKDPFAPSSSAKPPKTSSSGFADFTSFGNEEQQLAWAKRESEKAEQERLARLRRQEQEDLELAIALSKADMPA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAPLVPLS ------CCCCCCCHH	20.14	-
9	Phosphorylation	AAPLVPLSQQIPGGN CCCCCCHHHCCCCCC	17.61	19144319
30	Phosphorylation	YKQVDPAYTGRVGAS HCCCCHHHCCCCCHH	18.34	19144319
31	Phosphorylation	KQVDPAYTGRVGASE CCCCHHHCCCCCHHH	22.18	19144319
49	Phosphorylation	FLKKSGLSDIILGKI HHHHCCCCHHHHHHH	30.23	22942356
74	Phosphorylation	FLDKQGFYVALRLVA CCCCCCHHHHHHHHH	7.52	16452087
106	Phosphorylation	PPPKFHDTSSPLMAT CCCCCCCCCCCCEEC	23.64	26239621
107	Phosphorylation	PPKFHDTSSPLMATQ CCCCCCCCCCCEECC	35.70	23335269
108	Phosphorylation	PKFHDTSSPLMATQS CCCCCCCCCCEECCC	24.93	26824392
113	Phosphorylation	TSSPLMATQSSAETH CCCCCEECCCCCCCE	18.28	28833060
115	Phosphorylation	SPLMATQSSAETHWA CCCEECCCCCCCEEE	27.45	28833060
116	Phosphorylation	PLMATQSSAETHWAV CCEECCCCCCCEEEE	21.95	28833060
119	Phosphorylation	ATQSSAETHWAVRVE ECCCCCCCEEEEEHH	23.57	28833060
217	Phosphorylation	PPSKRKKTVFAGAVP CCHHCCCEEEECCEE	24.72	25619855
229	Phosphorylation	AVPVLPASPPPKDSL CEEECCCCCCCCCCC	35.68	24925903
235	Phosphorylation	ASPPPKDSLRSTPSH CCCCCCCCCCCCCCC	31.63	22942356
238	Phosphorylation	PPKDSLRSTPSHGSV CCCCCCCCCCCCCCC	50.38	24925903
238 (in isoform 2)	Phosphorylation	-	50.38	19144319
239	Phosphorylation	PKDSLRSTPSHGSVS CCCCCCCCCCCCCCC	23.52	24925903
241	Phosphorylation	DSLRSTPSHGSVSSL CCCCCCCCCCCCCCC	40.34	24925903
244	Phosphorylation	RSTPSHGSVSSLNST CCCCCCCCCCCCCCC	17.25	24925903
246	Phosphorylation	TPSHGSVSSLNSTGS CCCCCCCCCCCCCCC	30.70	24925903
247	Phosphorylation	PSHGSVSSLNSTGSL CCCCCCCCCCCCCCC	29.43	24925903
250	Phosphorylation	GSVSSLNSTGSLSPK CCCCCCCCCCCCCCC	39.16	24925903
251	Phosphorylation	SVSSLNSTGSLSPKH CCCCCCCCCCCCCCC	29.71	24925903
253	Phosphorylation	SSLNSTGSLSPKHSV CCCCCCCCCCCCCCC	26.26	24925903
255	Phosphorylation	LNSTGSLSPKHSVKQ CCCCCCCCCCCCCCC	33.33	24925903
255 (in isoform 2)	Phosphorylation	-	33.33	19144319
259	Phosphorylation	GSLSPKHSVKQPPVA CCCCCCCCCCCCCEE	36.82	-
291	Phosphorylation	TDLDLDGYVSGQEVK CCCCCCCCCCHHHHH	7.36	25195567
293	Phosphorylation	LDLDGYVSGQEVKEI CCCCCCCCHHHHHHH	26.69	26525534
304	Phosphorylation	VKEIFMHSGLTQNLL HHHHHHCCCHHHHHH	24.58	20495213
353	Phosphorylation	IDPPQVLSPDMVPPS CCCCCCCCCCCCCCH	21.72	25521595
360	Phosphorylation	SPDMVPPSERGTPIP CCCCCCCHHCCCCCC	33.58	26239621
364	Phosphorylation	VPPSERGTPIPDSSS CCCHHCCCCCCCCCC	25.02	25521595
369	Phosphorylation	RGTPIPDSSSTLASG CCCCCCCCCCCCCCC	22.34	28833060
370	Phosphorylation	GTPIPDSSSTLASGE CCCCCCCCCCCCCCC	34.42	28833060
371	Phosphorylation	TPIPDSSSTLASGEF CCCCCCCCCCCCCCC	31.24	25521595
372	Phosphorylation	PIPDSSSTLASGEFT CCCCCCCCCCCCCCC	28.43	28833060
375	Phosphorylation	DSSSTLASGEFTGVK CCCCCCCCCCCCCCC	41.80	26824392
379	Phosphorylation	TLASGEFTGVKELDD CCCCCCCCCCCCHHH	36.44	28833060
400	Phosphorylation	QLQREKYSLEQDIRE HHHHHHHCHHHHHHH	36.18	28066266
430	Phosphorylation	QNDLDRETSSLQELE HHHHHHHHHHHHHHH	25.22	28066266
431	Phosphorylation	NDLDRETSSLQELEA HHHHHHHHHHHHHHH	25.05	28066266
432	Phosphorylation	DLDRETSSLQELEAQ HHHHHHHHHHHHHHH	41.30	28066266
462	Phosphorylation	AKLRDMLSDVRQKCQ HHHHHHHHHHHHHHH	27.67	29514104
480	Phosphorylation	QTISSLKTQIQSQES HHHHHHHHHHHHHHH	35.88	18779572
484	Phosphorylation	SLKTQIQSQESDLKS HHHHHHHHHHHHHHH	37.23	18779572
487	Phosphorylation	TQIQSQESDLKSQED HHHHHHHHHHHHHHH	40.38	18779572
490	Ubiquitination	QSQESDLKSQEDDLN HHHHHHHHHHHHHHH	55.90	22790023
532	Ubiquitination	ETILRSLKCTQDDIN HHHHHHCCCCHHHHH	36.16	22790023
533	Glutathionylation	TILRSLKCTQDDINQ HHHHHCCCCHHHHHH	5.11	24333276
544	Ubiquitination	DINQARSKLSQLQES HHHHHHHHHHHHHHH	46.75	22790023
546	Phosphorylation	NQARSKLSQLQESHL HHHHHHHHHHHHHHH	32.36	29899451
558	Phosphorylation	SHLEAHRSLEQYDQV HHHHHHHCHHHHHCC	26.79	22499769
558 (in isoform 4)	Phosphorylation	-	26.79	29899451
562	Phosphorylation	AHRSLEQYDQVPDGV HHHCHHHHHCCCCCC	10.04	22499769
562 (in isoform 4)	Phosphorylation	-	10.04	29899451
570	Phosphorylation	DQVPDGVSGTSLPDL HCCCCCCCCCCCCCH	41.71	22499769
572	Phosphorylation	VPDGVSGTSLPDLAT CCCCCCCCCCCCHHH	21.42	22499769
573	Phosphorylation	PDGVSGTSLPDLATL CCCCCCCCCCCHHHH	41.24	22499769
579	Phosphorylation	TSLPDLATLNEGILL CCCCCHHHHCCCEEE	37.10	23984901
579 (in isoform 4)	Phosphorylation	-	37.10	29514104
600	Ubiquitination	GAMDDPFKNKALLFS CCCCCCCCCCEEEEC	64.03	22790023
602	Ubiquitination	MDDPFKNKALLFSNN CCCCCCCCEEEECCC	40.79	22790023
607	Phosphorylation	KNKALLFSNNSQELH CCCEEEECCCCCCCC	34.76	26239621
610	Phosphorylation	ALLFSNNSQELHPDP EEEECCCCCCCCCCC	29.31	26824392
610 (in isoform 2)	Phosphorylation	-	29.31	19144319
625	Ubiquitination	FQAEDPFKSDPFKGA CCCCCCCCCCCCCCC	60.38	-
626	Phosphorylation	QAEDPFKSDPFKGAD CCCCCCCCCCCCCCC	51.39	25293948
630	Ubiquitination	PFKSDPFKGADPFKG CCCCCCCCCCCCCCC	59.59	22790023
636	Ubiquitination	FKGADPFKGDPFQSD CCCCCCCCCCCCCCC	68.72	-
646	Phosphorylation	PFQSDPFSEQQTAAT CCCCCCCCHHHHCCC	39.62	23984901
650	Phosphorylation	DPFSEQQTAATDPFG CCCCHHHHCCCCCCC	20.25	23984901
653	Phosphorylation	SEQQTAATDPFGGDP CHHHHCCCCCCCCCC	41.56	23984901
664	Phosphorylation	GGDPFKESDPFHSSS CCCCCCCCCCCCCCC	51.69	27742792
669	Phosphorylation	KESDPFHSSSSDDFF CCCCCCCCCCHHHHH	32.09	27742792
670	Phosphorylation	ESDPFHSSSSDDFFK CCCCCCCCCHHHHHH	25.87	27742792
671	Phosphorylation	SDPFHSSSSDDFFKK CCCCCCCCHHHHHHH	40.58	27742792
672	Phosphorylation	DPFHSSSSDDFFKKQ CCCCCCCHHHHHHHH	43.05	27742792
677	Ubiquitination	SSSDDFFKKQTKNDP CCHHHHHHHHCCCCC	43.92	-
678	Ubiquitination	SSDDFFKKQTKNDPF CHHHHHHHHCCCCCC	59.03	-
681	Ubiquitination	DFFKKQTKNDPFTSD HHHHHHCCCCCCCCC	57.78	-
687	Phosphorylation	TKNDPFTSDPFTKNP CCCCCCCCCCCCCCC	43.60	18779572
692	Ubiquitination	FTSDPFTKNPSLPSK CCCCCCCCCCCCCCC	68.21	22790023
695	Phosphorylation	DPFTKNPSLPSKLDP CCCCCCCCCCCCCCC	63.41	30352176
699	Ubiquitination	KNPSLPSKLDPFESS CCCCCCCCCCCCCCC	55.77	22790023
706	Phosphorylation	KLDPFESSDPFSSSS CCCCCCCCCCCCCCC	41.95	26060331
713	Phosphorylation	SDPFSSSSISSKGSD CCCCCCCCCCCCCCC	28.56	26060331
715	Phosphorylation	PFSSSSISSKGSDPF CCCCCCCCCCCCCCC	28.13	26060331
732	Phosphorylation	LDPFGSSSFSSAEGF CCCCCCCCCCCCCCC	30.74	-
774	Phosphorylation	PFKSKQDTPALPPKK CCCCCCCCCCCCCCC	14.15	27600695
791	Phosphorylation	PPRPKPPSGQSTPVS CCCCCCCCCCCCCHH	58.97	29899451
794	Phosphorylation	PKPPSGQSTPVSQLG CCCCCCCCCCHHHCC	37.90	25521595
795	Phosphorylation	KPPSGQSTPVSQLGS CCCCCCCCCHHHCCC	21.59	29899451
798	Phosphorylation	SGQSTPVSQLGSSDF CCCCCCHHHCCCCCC	22.38	25521595
808	Phosphorylation	GSSDFPESPDPFQPL CCCCCCCCCCCCCCC	34.54	26060331
817 (in isoform 2)	Phosphorylation	-	18.58	27149854
827	Ubiquitination	GDPFQNKKGFGDPFS CCCCCCCCCCCCCCC	68.00	22790023
836	Ubiquitination	FGDPFSGKDPFAPSS CCCCCCCCCCCCCCC	62.11	22790023
846	Ubiquitination	FAPSSSAKPPKTSSS CCCCCCCCCCCCCCC	64.89	22790023
849	Ubiquitination	SSSAKPPKTSSSGFA CCCCCCCCCCCCCCC	70.35	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of EP15R_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EP15R_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EP15R_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AGFG1_HUMAN	AGFG1	physical	9303539
AGFG2_HUMAN	AGFG2	physical	9303539
NUMB_HUMAN	NUMB	physical	9303539
NUMBL_HUMAN	NUMBL	physical	9303539
PRP17_HUMAN	CDC40	physical	9303539
EPN2_HUMAN	EPN2	physical	9303539

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EP15R_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The phagosomal proteome in interferon-gamma-activated macrophages."; Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.; Immunity 30:143-154(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; TYR-30; THR-31;SER-238 AND SER-255, AND MASS SPECTROMETRY.	19144319 [Abstract]
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-244; SER-250 ANDSER-255, AND MASS SPECTROMETRY.	17242355 [Abstract]
"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations."; Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.; Mol. Cell. Proteomics 6:283-293(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229; SER-353 ANDTHR-364, AND MASS SPECTROMETRY.	17114649 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-74, AND MASSSPECTROMETRY.	16452087 [Abstract]