dbPTM

DPOLA_DROME - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	DPOLA_DROME
UniProt AC	P26019
Protein Name	DNA polymerase alpha catalytic subunit
Gene Name	DNApol-alpha180
Organism	Drosophila melanogaster (Fruit fly).
Sequence Length	1488
Subcellular Localization	Nucleus .
Protein Description	Polymerase alpha in a complex with DNA primase is a replicative polymerase. In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity..
Protein Sequence	MSESPSEPRAKRQRVDKNGRFAAMERLRQLKGTKNKCKVEDQVDDVYDVVDEREYAKRAQEKYGDDWIEEDGTGYAEDLRDFFEDEDEYSDGEEDRKDSKKKKGVAPNSKKRPRENEKPVTGKASIKNLFSNAVPKKMDVKTSVKDDDILADILGEIKEEPAATSEKAEKVIAPAKISVTSRKFDAAAAKEYMNSFLNNIKVQEQERKKAEASSDNEMLERILKPKAAVPNTKVAFFSSPTIKKEPMPEKTPAKKATEDPFSDNEMDFSCLDDDENQFDVEKTQQTEKVSQTKTAAEKTSQSKVAEKSAPKKETTGSPKESESEDISRLLNNWESICQMDDDFEKSVLTTEQDSTISSDQQLRFWYWEAYEDPVKMPGEVFLFGRTADGKSVCLRVQNINRVLYLLPRQFLLDPISKEPTKQKVTVADIYKEFDSEVANQLKLEFFRSRKVTKSFAHHAIGIEVPQSCDYLEVHYDGKKPLPNLSADKKYNSIAHIFGATTNALERFLLDRKIKGPCWLQVTGFKVSPTPMSWCNTEVTLTEPKNVELVQDKGKPAPPPPLTLLSLNVRTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMCALTRPAVVSWPLDLNFEMAKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQLNVITDQIVALKIPQWSRMGRLRLSQSFGKRLLEHFVGRMVCDVKRSAEECIRARSYDLQTLCKQVLKLKESERMEVNADDLLEMYEKGESITKLISLTMQDNSYLLRLMCELNIMPLALQITNICGNTMTRTLQGGRSERNEFLLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSGADATMQTKKKAAYAGGLVLEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTVQQPVDADELPTLPDSKTEPGILPLQLKRLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKGREILTNTQQLVQKMNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVNKLYKQLELDIDGVFGCLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLDAVHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRMNRERNRRYKKGDMVDYVICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLEGTDASRIAECLGMDPTKFRQNAQRTQRENTEQSEGESLLKTTLQLYRLCEPFRFQCVTCKTEQLMASAYRPGPSNSHIAVLQQCAKSECQTAPIQYLASVRNQLQLSMRQYVQRFYKNWLVCDHPDCNFNTRTHSLRKKSHRPLCQKCRSGSLLRQYTERDLYNQLCYLRFMFDLGKQTLQQKPTLTPELEQAYQLLYETVDQQLQSSSYVIISLSKLFARSLAQMSLQPSVAQPQIEAIPSALADVV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
89	Phosphorylation	FFEDEDEYSDGEEDR HHCCCCCCCCCCCHH	25.12	21082442
90	Phosphorylation	FEDEDEYSDGEEDRK HCCCCCCCCCCCHHH	36.77	19429919
214	Phosphorylation	RKKAEASSDNEMLER HHHHHHCCCHHHHHH	52.05	19429919
238	Phosphorylation	NTKVAFFSSPTIKKE CCEEEEEECCCCCCC	29.02	28490779
239	Phosphorylation	TKVAFFSSPTIKKEP CEEEEEECCCCCCCC	22.39	28490779
241	Phosphorylation	VAFFSSPTIKKEPMP EEEEECCCCCCCCCC	47.58	21082442
262	Phosphorylation	KATEDPFSDNEMDFS CCCCCCCCCCCCCCC	44.93	18327897
269	Phosphorylation	SDNEMDFSCLDDDEN CCCCCCCCCCCCCCC	14.98	18327897
290	Phosphorylation	TQQTEKVSQTKTAAE HHHHHCHHHHHHHHH	43.11	22817900
308	Phosphorylation	QSKVAEKSAPKKETT HHHHHHHCCCCCCCC	41.11	22668510
314	Phosphorylation	KSAPKKETTGSPKES HCCCCCCCCCCCCCC	45.39	19429919
315	Phosphorylation	SAPKKETTGSPKESE CCCCCCCCCCCCCCC	36.43	19429919
317	Phosphorylation	PKKETTGSPKESESE CCCCCCCCCCCCCHH	30.61	19429919
321	Phosphorylation	TTGSPKESESEDISR CCCCCCCCCHHHHHH	53.18	19429919
323	Phosphorylation	GSPKESESEDISRLL CCCCCCCHHHHHHHH	50.26	19429919

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of DPOLA_DROME !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of DPOLA_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of DPOLA_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IMA_DROME	Pen	physical	10882518
PRI1_DROME	DNApol-alpha50	physical	6403945
KMT5A_DROME	pr-set7	physical	24806961
PRI2_DROME	DNApol-alpha60	physical	6403945

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of DPOLA_DROME

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphoproteome analysis of Drosophila melanogaster embryos."; Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.; J. Proteome Res. 7:1675-1682(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239; SER-262; SER-269;THR-314 AND SER-317, AND MASS SPECTROMETRY.	18327897 [Abstract]