CNBL4_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CNBL4_ARATH
• UniProt AC: O81223
• Protein Name: Calcineurin B-like protein 4
• Gene Name: CBL4
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 222
• Subcellular Localization: Cell membrane ; Lipid-anchor . Cytoplasm . Nucleus . The cell membrane localization is S-acylation dependent.
• Protein Description: Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation..
• Protein Sequence: MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	N-myristoyl glycine	------MGCSVSKKK ------CCCCCCCCC	16.65	-
2	Myristoylation	------MGCSVSKKK ------CCCCCCCCC	16.65	18502848
205	Phosphorylation	DINRTFPSFVSSCEE HHHHHCHHHHHHHHH	33.80	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of CNBL4_ARATH !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CNBL4_ARATH !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CNBL4_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CIPKO_ARATH	SOS2	physical	10725350
CNBL4_ARATH	SOS3	physical	15644219
CIPKA_ARATH	SIP1	physical	11402167
CIPKF_ARATH	CIPK15	physical	11402167
CIPK9_ARATH	CIPK9	physical	11402167
CIPK7_ARATH	CIPK7	physical	11402167
CDPK1_ARATH	CPK1	physical	22737156
CIPKO_ARATH	SOS2	physical	22253446
GIGAN_ARATH	GI	physical	23322040
CIPKO_ARATH	SOS2	physical	11006339

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Myristoylation

"SOS3 function in plant salt tolerance requires N-myristoylation andcalcium binding.";
Ishitani M., Liu J., Halfter U., Kim C.-S., Shi W., Zhu J.-K.;
Plant Cell 12:1667-1677(2000).
Cited for: FUNCTION, MYRISTOYLATION AT GLY-2, AND MUTAGENESIS OF GLY-2.
PubMed: 11006339