dbPTM

CCD50_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CCD50_HUMAN
UniProt AC	Q8IVM0
Protein Name	Coiled-coil domain-containing protein 50
Gene Name	CCDC50
Organism	Homo sapiens (Human).
Sequence Length	306
Subcellular Localization	Cytoplasm. Associated with microtubules of the cytoskeleton and mitotic apparatus..
Protein Description	Involved in EGFR signaling..
Protein Sequence	MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQAQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYTHFTNQQSSTRHFSKSESSHKGFHYKH

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAEVSIDQS ------CCCCCCCHH	26.37	22814378
5	Phosphorylation	---MAEVSIDQSKLP ---CCCCCCCHHHCC	16.38	30108239
9	Phosphorylation	AEVSIDQSKLPGVKE CCCCCCHHHCCCHHH	32.20	27251275
15	Ubiquitination	QSKLPGVKEVCRDFA HHHCCCHHHHHHHHH	50.79	33845483
28	Phosphorylation	FAVLEDHTLAHSLQE HHHHCCCCHHHHHHH	37.65	27080861
32	Phosphorylation	EDHTLAHSLQEQEIE CCCCHHHHHHHHHHH	26.58	29255136
60	Ubiquitination	QHDLQVAKQLQEEDL HHHHHHHHHHCHHHH	53.32	22053931
60 (in isoform 2)	Ubiquitination	-	53.32	21890473
60	Acetylation	QHDLQVAKQLQEEDL HHHHHHHHHHCHHHH	53.32	26051181
60 (in isoform 1)	Ubiquitination	-	53.32	21890473
68	Ubiquitination	QLQEEDLKAQAQLQK HHCHHHHHHHHHHHH	51.26	32015554
68 (in isoform 1)	Ubiquitination	-	51.26	21890473
68 (in isoform 2)	Ubiquitination	-	51.26	21890473
75	Ubiquitination	KAQAQLQKRYKDLEQ HHHHHHHHHHHHHHH	67.31	32142685
77	Phosphorylation	QAQLQKRYKDLEQQD HHHHHHHHHHHHHHH	18.85	27067055
78	Ubiquitination	AQLQKRYKDLEQQDC HHHHHHHHHHHHHHH	60.93	29967540
78	Acetylation	AQLQKRYKDLEQQDC HHHHHHHHHHHHHHH	60.93	27452117
94	Ubiquitination	IAQEIQEKLAIEAER HHHHHHHHHHHHHHH	27.18	21963094
107	Ubiquitination	ERRRIQEKKDEDIAR HHHHHHHHCHHHHHH	49.50	22817900
108	Ubiquitination	RRRIQEKKDEDIARL HHHHHHHCHHHHHHH	66.65	21963094
108 (in isoform 1)	Ubiquitination	-	66.65	21890473
108 (in isoform 2)	Ubiquitination	-	66.65	21890473
119	Ubiquitination	IARLLQEKELQEEKK HHHHHHHHHHHHHHH	51.25	32142685
119 (in isoform 1)	Ubiquitination	-	51.25	21890473
119 (in isoform 2)	Ubiquitination	-	51.25	21890473
125	Ubiquitination	EKELQEEKKRKKHFP HHHHHHHHHHHHCCC	58.06	23000965
126	Ubiquitination	KELQEEKKRKKHFPE HHHHHHHHHHHCCCC	73.13	23000965
128	Ubiquitination	LQEEKKRKKHFPEFP HHHHHHHHHCCCCCH	60.52	23000965
129	Ubiquitination	QEEKKRKKHFPEFPA HHHHHHHHCCCCCHH	55.28	23000965
129 (in isoform 1)	Ubiquitination	-	55.28	21890473
129 (in isoform 2)	Ubiquitination	-	55.28	21890473
137	Phosphorylation	HFPEFPATRAYADSY CCCCCHHHHHHCCCE	18.97	27470641
140	Phosphorylation	EFPATRAYADSYYYE CCHHHHHHCCCEECC	13.98	21945579
143	Phosphorylation	ATRAYADSYYYEDGG HHHHHCCCEECCCCC	13.58	21945579
144	Phosphorylation	TRAYADSYYYEDGGM HHHHCCCEECCCCCC	15.60	21945579
144 (in isoform 2)	Phosphorylation	-	15.60	17016520
145	Phosphorylation	RAYADSYYYEDGGMK HHHCCCEECCCCCCC	12.34	21945579
145 (in isoform 2)	Phosphorylation	-	12.34	15951569
146	Phosphorylation	AYADSYYYEDGGMKP HHCCCEECCCCCCCC	10.18	21945579
152	Ubiquitination	YYEDGGMKPRVMKEA ECCCCCCCCHHHHHH	32.71	23000965
152	Neddylation	YYEDGGMKPRVMKEA ECCCCCCCCHHHHHH	32.71	32015554
152 (in isoform 1)	Ubiquitination	-	32.71	21890473
154 (in isoform 2)	Phosphorylation	-	35.33	27251275
157	Ubiquitination	GMKPRVMKEAVSTPS CCCCHHHHHHHCCHH	39.40	23000965
157 (in isoform 1)	Ubiquitination	-	39.40	21890473
161	Phosphorylation	RVMKEAVSTPSRMAH HHHHHHHCCHHHHHH	41.47	21815630
162	Phosphorylation	VMKEAVSTPSRMAHR HHHHHHCCHHHHHHC	20.77	21815630
164	Phosphorylation	KEAVSTPSRMAHRDQ HHHHCCHHHHHHCCH	34.47	30576142
174	Phosphorylation	AHRDQEWYDAEIARK HHCCHHHHHHHHHHH	12.43	21945579
181	Ubiquitination	YDAEIARKLQEEELL HHHHHHHHHHHHHHH	45.56	21963094
181 (in isoform 1)	Ubiquitination	-	45.56	21890473
190	Phosphorylation	QEEELLATQVDMRAA HHHHHHHHHHHHHHH	29.15	27470641
203 (in isoform 2)	Phosphorylation	-	38.94	-
205 (in isoform 2)	Phosphorylation	-	2.25	26657352
206 (in isoform 2)	Phosphorylation	-	10.19	28985074
208 (in isoform 2)	Phosphorylation	-	2.57	28985074
209 (in isoform 2)	Phosphorylation	-	2.26	-
211	Ubiquitination	EIARLLMAEEKKAYK HHHHHHHHHHHHHHH	23.05	27667366
214	Ubiquitination	RLLMAEEKKAYKKAK HHHHHHHHHHHHHHH	33.92	27667366
214 (in isoform 1)	Ubiquitination	-	33.92	21890473
215	Ubiquitination	LLMAEEKKAYKKAKE HHHHHHHHHHHHHHH	61.29	22817900
217	Phosphorylation	MAEEKKAYKKAKERE HHHHHHHHHHHHHHH	23.15	19059208
218	Acetylation	AEEKKAYKKAKEREK HHHHHHHHHHHHHHH	51.63	7704397
218	Ubiquitination	AEEKKAYKKAKEREK HHHHHHHHHHHHHHH	51.63	22817900
219	Acetylation	EEKKAYKKAKEREKS HHHHHHHHHHHHHHH	52.60	7704409
219	Ubiquitination	EEKKAYKKAKEREKS HHHHHHHHHHHHHHH	52.60	22817900
221	Acetylation	KKAYKKAKEREKSSL HHHHHHHHHHHHHHH	67.21	7704421
221	Ubiquitination	KKAYKKAKEREKSSL HHHHHHHHHHHHHHH	67.21	23503661
225	Ubiquitination	KKAKEREKSSLDKRK HHHHHHHHHHHHHHH	52.12	25015289
225 (in isoform 1)	Ubiquitination	-	52.12	21890473
227	Phosphorylation	AKEREKSSLDKRKQD HHHHHHHHHHHHHCC	52.21	27470641
230	Ubiquitination	REKSSLDKRKQDPEW HHHHHHHHHHCCCCC	67.78	22817900
232	Ubiquitination	KSSLDKRKQDPEWKP HHHHHHHHCCCCCCH	65.87	27667366
232 (in isoform 1)	Ubiquitination	-	65.87	21890473
238	Sumoylation	RKQDPEWKPKTAKAA HHCCCCCCHHHHHHH	34.67	-
238	Ubiquitination	RKQDPEWKPKTAKAA HHCCCCCCHHHHHHH	34.67	27667366
238	Sumoylation	RKQDPEWKPKTAKAA HHCCCCCCHHHHHHH	34.67	-
238 (in isoform 1)	Ubiquitination	-	34.67	21890473
240	Ubiquitination	QDPEWKPKTAKAANS CCCCCCHHHHHHHCC	58.34	21963094
241	O-linked_Glycosylation	DPEWKPKTAKAANSK CCCCCHHHHHHHCCC	41.75	30379171
241	Phosphorylation	DPEWKPKTAKAANSK CCCCCHHHHHHHCCC	41.75	27470641
243	Ubiquitination	EWKPKTAKAANSKSK CCCHHHHHHHCCCCC	53.95	22817900
247	O-linked_Glycosylation	KTAKAANSKSKESDE HHHHHHCCCCCCCCC	33.31	30379171
249	O-linked_Glycosylation	AKAANSKSKESDEPH HHHHCCCCCCCCCCC	41.29	30379171
250	Ubiquitination	KAANSKSKESDEPHH HHHCCCCCCCCCCCC	66.04	29967540
250 (in isoform 1)	Ubiquitination	-	66.04	21890473
259	Ubiquitination	SDEPHHSKNERPARP CCCCCCCCCCCCCCC	59.47	21963094
272	Phosphorylation	RPPPPIMTDGEDADY CCCCCCCCCCCCCCC	41.39	21945579
276 (in isoform 2)	Phosphorylation	-	50.71	27794612
279	Phosphorylation	TDGEDADYTHFTNQQ CCCCCCCCCCCCCCC	12.11	21945579
280	Phosphorylation	DGEDADYTHFTNQQS CCCCCCCCCCCCCCC	15.85	21945579
280 (in isoform 2)	Phosphorylation	-	15.85	-
282	Ubiquitination	EDADYTHFTNQQSST CCCCCCCCCCCCCCC	5.45	27667366
283	Phosphorylation	DADYTHFTNQQSSTR CCCCCCCCCCCCCCC	25.23	21945579
287	Phosphorylation	THFTNQQSSTRHFSK CCCCCCCCCCCCCCC	24.74	21945579
288	Phosphorylation	HFTNQQSSTRHFSKS CCCCCCCCCCCCCCC	25.73	21945579
289	Phosphorylation	FTNQQSSTRHFSKSE CCCCCCCCCCCCCCC	33.15	21945579
292 (in isoform 2)	Phosphorylation	-	9.91	28152594
293	Phosphorylation	QSSTRHFSKSESSHK CCCCCCCCCCCCCCC	29.19	24719451
294	Ubiquitination	SSTRHFSKSESSHKG CCCCCCCCCCCCCCC	58.25	25015289
300	Ubiquitination	SKSESSHKGFHYKH- CCCCCCCCCCCCCC-	66.28	21963094
304	Phosphorylation	SSHKGFHYKH----- CCCCCCCCCC-----	14.43	19059208
310 (in isoform 2)	Phosphorylation	-		26657352
314 (in isoform 2)	Phosphorylation	-		24275569
328	Ubiquitination	----------------------------- -----------------------------		23000965
333	Ubiquitination	---------------------------------- ----------------------------------		23000965
333 (in isoform 2)	Ubiquitination	-		21890473
357	Ubiquitination	---------------------------------------------------------- ----------------------------------------------------------		21963094
357 (in isoform 2)	Ubiquitination	-		21890473
390	Ubiquitination	------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------		27667366
390 (in isoform 2)	Ubiquitination	-		21890473
391	Ubiquitination	-------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------		22817900
394	Ubiquitination	----------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------		22817900
395	Ubiquitination	------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------		22817900
397	Ubiquitination	-------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------		23503661
401	Ubiquitination	------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------		25015289
401 (in isoform 2)	Ubiquitination	-		21890473
406	Ubiquitination	----------------------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------------------		22817900
408	Ubiquitination	------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------		27667366
408 (in isoform 2)	Ubiquitination	-		21890473
414	Ubiquitination	------------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------------		27667366
414 (in isoform 2)	Ubiquitination	-		21890473
416	Ubiquitination	--------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------		21963094
419	Ubiquitination	------------------------------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------------------------------		22817900
426	Ubiquitination	------------------------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------------------------		29967540
426 (in isoform 2)	Ubiquitination	-		21890473
435	Ubiquitination	---------------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------------		21963094
470	Ubiquitination	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------		25015289
476	Ubiquitination	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		21963094

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
217	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
217	Y	Phosphorylation	Kinase	LCK	P06239	PSP
279	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
279	Y	Phosphorylation	Kinase	LCK	P06239	PSP
304	Y	Phosphorylation	Kinase	EGFR	P00533	PSP
304	Y	Phosphorylation	Kinase	LCK	P06239	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CCD50_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CCD50_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EGFR_HUMAN	EGFR	physical	16803894
UBC_HUMAN	UBC	physical	18029035
TNAP3_HUMAN	TNFAIP3	physical	18029035
RIPK1_HUMAN	RIPK1	physical	18029035
G3PT_HUMAN	GAPDHS	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
607453	Deafness, autosomal dominant, 44 (DFNA44)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CCD50_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.	19413330 [Abstract]
Phosphorylation
Reference	PubMed
"An extensive survey of tyrosine phosphorylation revealing new sitesin human mammary epithelial cells."; Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A.,Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D.,Wiley H.S., Qian W.-J.; J. Proteome Res. 8:3852-3861(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-144, AND MASSSPECTROMETRY.	19534553 [Abstract]
"Multiple reaction monitoring for robust quantitative proteomicanalysis of cellular signaling networks."; Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M.; Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-144 AND TYR-279, ANDMASS SPECTROMETRY.	17389395 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-144 AND TYR-279, ANDMASS SPECTROMETRY.	17081983 [Abstract]
"Time-resolved mass spectrometry of tyrosine phosphorylation sites inthe epidermal growth factor receptor signaling network reveals dynamicmodules."; Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J.,Lauffenburger D.A., White F.M.; Mol. Cell. Proteomics 4:1240-1250(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-140 AND TYR-145, ANDMASS SPECTROMETRY.	15951569 [Abstract]