BUD5_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BUD5_YEAST
• UniProt AC: P25300
• Protein Name: Bud site selection protein 5
• Gene Name: BUD5
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 642
• Subcellular Localization: Bud neck. Cytoplasm, cell cortex. In haploid cells, forms double rings that encircle the mother-bud neck and split upon cytokinesis, each progeny cell inheriting BUD5 at the axial division remnant. In diploid cells, localizes to the poles of the cell
• Protein Description: GDP-GTP exchange factor (GEF) for the small GTPase BUD1/RSR1. Regulates the activity of BUD1 together with BUD2 which is a GTPase-activating protein (GAP) of BUD1. Required to produce both the axial and bipolar patterns of bud site selection. Determines the orientation of division axis. Overexpression can suppress mutations in PRP20 which is the GEF for GSP1. May be a cytoplasmic GEF for GSP1. Might also act on the Ras-like protein CDC42. Appears to bind to Ras proteins but not to activate them..
• Protein Sequence: MSPKNKYVYICVEYIYIYFAKIHKQSTLSSDTTKMFVLIDNVLAYLLEQDDLFVTARFAIQGQIVSRRVNKIHISNITDVLLQQFISHTLPYNDNIVPKKILDSMRTAVRQLLEATACVSRECPLVKRSQDIKRARKRLLSDWYRLGADANMDAVLLVVNSAWRFLAVWRPFVNSIQHATQELYQNIAHYLLHGNVNIQRVTALIQLVMGQDDLLFSMDDVLQEVFRIQLYLNKMLPHNSHKWQKPSPFDSANLLLNFRDWTTDNALLQELLLSYPTINKNKHKNHSVPRLIQIWVESYWQDSETTLKDILNFWYSHLAEYYEYQELFADIVQLFINKKRTRQLKIHYIGLTDKEIEENKPPLDYENLFLQYEIDKTNANDELCGATDLSDLLFQWKQGELLEVEAFALNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDTTIDSVFTLSNQLSSYVLETTLQQTHTISYWLQVALSCLYLRNLNSLASIITSLQNHSIERLSLPIDVKSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSQLPCVPFTSLLIRDITFIRDGNDTFTKDGNNVNMQKFNQITKIVAFAQYLQQKQYEDIHCSNTTARSLLGAMIKVHTLYNDNKDRAYQVSIAKVPRLT

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
507	Phosphorylation	NHSIERLSLPIDVKS CCCCEEECCCCCCCC	38.02	28889911
571	Ubiquitination	DGNDTFTKDGNNVNM CCCCCCCCCCCCCCH	60.49	23749301
637	Ubiquitination	AYQVSIAKVPRLT-- EEEEEEECCCCCC--	51.61	23749301

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of BUD5_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BUD5_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BUD5_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GSP1_YEAST	GSP1	physical	11589573
BUD8_YEAST	BUD8	physical	17581861
BUD9_YEAST	BUD9	physical	17581861
AXL2_YEAST	AXL2	physical	22553209
AXL1_YEAST	AXL1	physical	22553209
BUD4_YEAST	BUD4	physical	22553209
BUD3_YEAST	BUD3	physical	22553209
BUD4_YEAST	BUD4	physical	25002677
AXL1_YEAST	AXL1	physical	25002677

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.