BLNK_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: BLNK_MOUSE
• UniProt AC: Q9QUN3
• Protein Name: B-cell linker protein
• Gene Name: Blnk
• Organism: Mus musculus (Mouse).
• Sequence Length: 457
• Subcellular Localization: Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction..
• Protein Description: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis..
• Protein Sequence: MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKPSWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRSTKPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAERHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPRFPEAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYFGSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
12	Phosphorylation	NKITVPASQKLRQLQ CCCCCCHHHHHHHHH	23.06	27841257
37	Ubiquitination	GGIMDKIKKLKVKGP CCHHHHHHHCCCCCC	58.77	-
38	Ubiquitination	GIMDKIKKLKVKGPP CHHHHHHHCCCCCCC	57.95	-
42	Ubiquitination	KIKKLKVKGPPSVPR HHHHCCCCCCCCCCC	65.20	-
72	Phosphorylation	SDDFDSDYENPDEHS CCCCCCCCCCCCCCC	22.89	22817900
84	Phosphorylation	EHSDSEMYVMPAEET CCCCCCEEEEECHHH	6.91	22817900
96	Phosphorylation	EETGDDSYEPPPAEQ HHHCCCCCCCCCHHH	39.18	22334673
115	Phosphorylation	VHPALPFTRGEYVDN EECCCCCCCCCCCCC	35.51	25367039
119	Phosphorylation	LPFTRGEYVDNRSSQ CCCCCCCCCCCCCCC	18.87	25367039
124	Phosphorylation	GEYVDNRSSQRHSPP CCCCCCCCCCCCCCC	36.76	20531401
125	Phosphorylation	EYVDNRSSQRHSPPF CCCCCCCCCCCCCCC	28.17	27555448
129	Phosphorylation	NRSSQRHSPPFSKTL CCCCCCCCCCCCCCC	36.14	26824392
133	Phosphorylation	QRHSPPFSKTLPSKP CCCCCCCCCCCCCCC	30.42	20531401
135	Phosphorylation	HSPPFSKTLPSKPSW CCCCCCCCCCCCCCC	42.58	24704852
141	Phosphorylation	KTLPSKPSWPSAKAR CCCCCCCCCHHHHHH	56.19	24719451
144	Phosphorylation	PSKPSWPSAKARLAS CCCCCCHHHHHHHHH	35.82	24719451
152	Phosphorylation	AKARLASTLPAPNSL HHHHHHHCCCCCCCC	31.02	22334673
158	Phosphorylation	STLPAPNSLQKPQVP HCCCCCCCCCCCCCC	30.99	27841257
178	Phosphorylation	LLEDEADYVVPVEDN HCCCCCCEEEECCCC	15.47	25159016
189	Phosphorylation	VEDNDENYIHPRESS CCCCCCCCCCCCCCC	9.88	25159016
195	Phosphorylation	NYIHPRESSPPPAEK CCCCCCCCCCCCHHH	49.80	26239621
196	Phosphorylation	YIHPRESSPPPAEKA CCCCCCCCCCCHHHC	36.35	26239621
220	Phosphorylation	NSSSKHMSPPGTVAG CCCCCCCCCCCCCCC	27.88	22942356
224	Phosphorylation	KHMSPPGTVAGRNSG CCCCCCCCCCCCCCC	16.98	24719451
230	Phosphorylation	GTVAGRNSGVWDSKS CCCCCCCCCCCCCCC	33.10	27180971
235	Phosphorylation	RNSGVWDSKSSLPAA CCCCCCCCCCCCCCC	20.80	-
244	Phosphorylation	SSLPAAPSPLPRAGK CCCCCCCCCCCCCCC	34.05	27180971
294	Phosphorylation	HRQDTVQSPVFPPTQ CCCCCCCCCCCCCCC	20.77	27841257
376	Phosphorylation	SFLIRKSSGHDSKQP CEEEEECCCCCCCCC	43.09	26102028

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
72	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot
84	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot
96	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot
152	T	Phosphorylation	Kinase	MAP4K1	Q92918	GPS
178	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot
189	Y	Phosphorylation	Kinase	SYK	P48025	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of BLNK_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of BLNK_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
M4K1_MOUSE	Map4k1	physical	11487585
CKLF3_MOUSE	Cmtm3	physical	15087455
BTK_MOUSE	Btk	physical	15087455
MK01_MOUSE	Mapk1	physical	15087455

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.