ATG4C_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ATG4C_HUMAN
• UniProt AC: Q96DT6
• Protein Name: Cysteine protease ATG4C
• Gene Name: ATG4C
• Organism: Homo sapiens (Human).
• Sequence Length: 458
• Subcellular Localization: Cytoplasm.
• Protein Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms..
• Protein Sequence: MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDHVIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALNIENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLALFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFKRASEEITKMLKFSSKEKYPLFTFVNGHSRDYDFTSTTTNEEDLFSEDEKKQLKRFSTEEFVLL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEATGTDE -------CCCCCCHH	7.74	19413330
11	Ubiquitination	TGTDEVDKLKTKFIS CCCHHHHHHHHHHHH	57.99	-
11	Acetylation	TGTDEVDKLKTKFIS CCCHHHHHHHHHHHH	57.99	25953088
15	Ubiquitination	EVDKLKTKFISAWNN HHHHHHHHHHHHHHC	38.83	-
18	Phosphorylation	KLKTKFISAWNNMKY HHHHHHHHHHHCCCE	30.29	-
25	Phosphorylation	SAWNNMKYSWVLKTK HHHHCCCEEEEEECC	9.30	25072903
26	Phosphorylation	AWNNMKYSWVLKTKT HHHCCCEEEEEECCC	12.75	25072903
31	Phosphorylation	KYSWVLKTKTYFSRN CEEEEEECCCCCCCC	25.18	25072903
33	Phosphorylation	SWVLKTKTYFSRNSP EEEEECCCCCCCCCC	34.72	25072903
34	Phosphorylation	WVLKTKTYFSRNSPV EEEECCCCCCCCCCE	10.91	25072903
36	Phosphorylation	LKTKTYFSRNSPVLL EECCCCCCCCCCEEE	21.82	25072903
144	Phosphorylation	DALNIENSDSESWTS CCCCCCCCCCCCCCC	29.71	28348404
158	Phosphorylation	SHTVKKFTASFEASL CCEEEEEEEEEEHHH	30.21	24719451
160	Phosphorylation	TVKKFTASFEASLSG EEEEEEEEEEHHHCC	22.14	26471730
164	Phosphorylation	FTASFEASLSGEREF EEEEEEHHHCCCCCC	18.83	24719451
166	Phosphorylation	ASFEASLSGEREFKT EEEEHHHCCCCCCCC	35.79	24719451
173	Phosphorylation	SGEREFKTPTISLKE CCCCCCCCCEEEHHH	30.87	28555341
179	Ubiquitination	KTPTISLKETIGKYS CCCEEEHHHHHHCCC	46.12	-
181	Phosphorylation	PTISLKETIGKYSDD CEEEHHHHHHCCCCC	33.18	29449344
185	Phosphorylation	LKETIGKYSDDHEMR HHHHHHCCCCCHHHH	16.48	29449344
186	Phosphorylation	KETIGKYSDDHEMRN HHHHHCCCCCHHHHH	39.79	23898821
364	Phosphorylation	IKDFPLETFHCPSPK ECCCCCCEECCCCCC	26.77	30266825
369	Phosphorylation	LETFHCPSPKKMSFR CCEECCCCCCCCCCC	55.54	30266825
374	Phosphorylation	CPSPKKMSFRKMDPS CCCCCCCCCCCCCCC	30.68	24719451
381	Phosphorylation	SFRKMDPSCTIGFYC CCCCCCCCCEEEEEE	21.45	21214269
383	Phosphorylation	RKMDPSCTIGFYCRN CCCCCCCEEEEEECC	28.59	-
387	Phosphorylation	PSCTIGFYCRNVQDF CCCEEEEEECCHHHH	5.81	21214269
398	Phosphorylation	VQDFKRASEEITKML HHHHHHHHHHHHHHH	39.53	27251275
403	Ubiquitination	RASEEITKMLKFSSK HHHHHHHHHHHCCCC	48.15	-
413	Phosphorylation	KFSSKEKYPLFTFVN HCCCCCCCCEEEEEC	13.27	26074081
417	Phosphorylation	KEKYPLFTFVNGHSR CCCCCEEEEECCCCC	34.06	26074081
423	Phosphorylation	FTFVNGHSRDYDFTS EEEECCCCCCCCCCC	28.39	26074081
426	Phosphorylation	VNGHSRDYDFTSTTT ECCCCCCCCCCCCCC	16.31	26074081
429	Phosphorylation	HSRDYDFTSTTTNEE CCCCCCCCCCCCCHH	23.43	26074081
430	Phosphorylation	SRDYDFTSTTTNEED CCCCCCCCCCCCHHH	24.61	26074081
431	Phosphorylation	RDYDFTSTTTNEEDL CCCCCCCCCCCHHHC	34.28	26074081
432	Phosphorylation	DYDFTSTTTNEEDLF CCCCCCCCCCHHHCC	27.65	26074081
433	Phosphorylation	YDFTSTTTNEEDLFS CCCCCCCCCHHHCCC	40.50	26074081
440	Phosphorylation	TNEEDLFSEDEKKQL CCHHHCCCHHHHHHH	51.97	28450419
451	Phosphorylation	KKQLKRFSTEEFVLL HHHHHCCCCCCEECC	38.65	27273156
452	Phosphorylation	KQLKRFSTEEFVLL- HHHHCCCCCCEECC-	36.50	22617229

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ATG4C_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ATG4C_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ATG4C_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SPTC1_HUMAN	SPTLC1	physical	20562859
PSMD2_HUMAN	PSMD2	physical	20562859
PRS7_HUMAN	PSMC2	physical	20562859
PRS4_HUMAN	PSMC1	physical	20562859
UBP11_HUMAN	USP11	physical	20562859
PRKDC_HUMAN	PRKDC	physical	20562859
PRS8_HUMAN	PSMC5	physical	20562859
PRS6B_HUMAN	PSMC4	physical	20562859
SMC1A_HUMAN	SMC1A	physical	20562859

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
PubMed: 19413330