AT1A3_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AT1A3_RAT
• UniProt AC: P06687
• Protein Name: Sodium/potassium-transporting ATPase subunit alpha-3
• Gene Name: Atp1a3
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1013
• Subcellular Localization: Cell membrane; Multi-pass membrane protein.
• Protein Description: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients..
• Protein Sequence: MGDKKDDKSSPKKSKAKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHGCKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVATGDRTVMGRIATLASGLEVGKTPIAIEIEHFIQLITGVAVFLGVSFFILSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGAPERILDRCATILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLISMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQRKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILRRNPGGWVEKETYY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	GDKKDDKSSPKKSKA CCCCCCCCCCCHHHH	59.89	-
26	Ubiquitination	RRDLDDLKKEVAMTE HCCHHHHHHHHHHHH	54.61	-
26	Acetylation	RRDLDDLKKEVAMTE HCCHHHHHHHHHHHH	54.61	22902405
27	Ubiquitination	RDLDDLKKEVAMTEH CCHHHHHHHHHHHHH	65.79	-
37	Phosphorylation	AMTEHKMSVEEVCRK HHHHHCCCHHHHHHH	31.05	25403869
45	Phosphorylation	VEEVCRKYNTDCVQG HHHHHHHHCCCCCCC	12.16	-
49	S-nitrosylation	CRKYNTDCVQGLTHS HHHHCCCCCCCCCHH	2.01	16418269
49	S-nitrosocysteine	CRKYNTDCVQGLTHS HHHHCCCCCCCCCHH	2.01	-
54	Phosphorylation	TDCVQGLTHSKAQEI CCCCCCCCHHHHHHH	30.81	25403869
56	Phosphorylation	CVQGLTHSKAQEILA CCCCCCHHHHHHHHH	25.17	25403869
57	Acetylation	VQGLTHSKAQEILAR CCCCCHHHHHHHHHC	46.62	22902405
57	Ubiquitination	VQGLTHSKAQEILAR CCCCCHHHHHHHHHC	46.62	-
146	Acetylation	YQEAKSSKIMESFKN HHHHHHHHHHHHHHH	53.84	42499145
146	Ubiquitination	YQEAKSSKIMESFKN HHHHHHHHHHHHHHH	53.84	-
152	Acetylation	SKIMESFKNMVPQQA HHHHHHHHHCCCCCE	54.11	7767455
152	Ubiquitination	SKIMESFKNMVPQQA HHHHHHHHHCCCCCE	54.11	-
184	Ubiquitination	VGDLVEIKGGDRVPA EECEEEECCCCCCCC	43.25	-
197	Phosphorylation	PADLRIISAHGCKVD CCCEEEEEECCCEEC	16.31	25403869
202	Ubiquitination	IISAHGCKVDNSSLT EEEECCCEECCCCCC	58.16	-
206	Phosphorylation	HGCKVDNSSLTGESE CCCEECCCCCCCCCC	23.43	29779826
207	Phosphorylation	GCKVDNSSLTGESEP CCEECCCCCCCCCCC	35.88	30411139
209	Phosphorylation	KVDNSSLTGESEPQT EECCCCCCCCCCCCC	40.63	27097102
212	Phosphorylation	NSSLTGESEPQTRSP CCCCCCCCCCCCCCC	56.79	27097102
216	Phosphorylation	TGESEPQTRSPDCTH CCCCCCCCCCCCCCC	43.78	28551015
218	Phosphorylation	ESEPQTRSPDCTHDN CCCCCCCCCCCCCCC	28.60	30411139
265	Phosphorylation	GRIATLASGLEVGKT HHHHHHHHCCCCCCC	47.20	-
356	Phosphorylation	KNLEAVETLGSTSTI CCHHHHHHHCCCCCC	29.80	28551015
359	Phosphorylation	EAVETLGSTSTICSD HHHHHHCCCCCCCCC	23.46	28551015
360	Phosphorylation	AVETLGSTSTICSDK HHHHHCCCCCCCCCC	28.26	28551015
361	Phosphorylation	VETLGSTSTICSDKT HHHHCCCCCCCCCCC	19.92	28551015
362	Phosphorylation	ETLGSTSTICSDKTG HHHCCCCCCCCCCCC	26.54	28551015
364	S-nitrosylation	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	16418269
364	S-nitrosocysteine	LGSTSTICSDKTGTL HCCCCCCCCCCCCCC	4.33	-
365	Phosphorylation	GSTSTICSDKTGTLT CCCCCCCCCCCCCCC	37.99	28551015
368	Phosphorylation	STICSDKTGTLTQNR CCCCCCCCCCCCCCC	40.15	22108457
370	Phosphorylation	ICSDKTGTLTQNRMT CCCCCCCCCCCCCEE	31.68	28551015
372	Phosphorylation	SDKTGTLTQNRMTVA CCCCCCCCCCCEEEE	24.52	28551015
377	Phosphorylation	TLTQNRMTVAHMWFD CCCCCCEEEEHHEEC	16.00	25403869
424	Acetylation	LCNRAVFKGGQDNIP HHCCHHHCCCCCCCC	56.38	22902405
424	Ubiquitination	LCNRAVFKGGQDNIP HHCCHHHCCCCCCCC	56.38	-
434	Acetylation	QDNIPVLKRDVAGDA CCCCCEECCCCCCCC	46.95	22902405
434	Ubiquitination	QDNIPVLKRDVAGDA CCCCCEECCCCCCCC	46.95	-
442	Phosphorylation	RDVAGDASESALLKC CCCCCCCCHHHHHHH	36.50	25403869
444	Phosphorylation	VAGDASESALLKCIE CCCCCCHHHHHHHHH	23.01	22673903
448	Ubiquitination	ASESALLKCIELSSG CCHHHHHHHHHHCCC	33.73	-
456	Phosphorylation	CIELSSGSVKLMRER HHHHCCCCHHHHHHH	20.30	25403869
458	Ubiquitination	ELSSGSVKLMRERNK HHCCCCHHHHHHHCC	37.96	-
474	Phosphorylation	VAEIPFNSTNKYQLS EEECCCCCCCCEEEE	33.46	28551015
475	Phosphorylation	AEIPFNSTNKYQLSI EECCCCCCCCEEEEE	36.51	28551015
477	Acetylation	IPFNSTNKYQLSIHE CCCCCCCCEEEEEEE	34.17	72620225
532	Phosphorylation	KEAFQNAYLELGGLG HHHHHHHHHHHCCHH	14.30	-
548	Phosphorylation	RVLGFCHYYLPEEQF HHHHHHCEECCHHHC	14.10	-
549	Phosphorylation	VLGFCHYYLPEEQFP HHHHHCEECCHHHCC	7.66	-
580	Phosphorylation	LCFVGLMSMIDPPRA CEEEHHHHCCCCCHH	20.03	-
595	Ubiquitination	AVPDAVGKCRSAGIK CCCCHHHHHHHCCCE	21.63	-
602	Ubiquitination	KCRSAGIKVIMVTGD HHHHCCCEEEEEECC	25.45	-
602	Acetylation	KCRSAGIKVIMVTGD HHHHCCCEEEEEECC	25.45	-
615	Ubiquitination	GDHPITAKAIAKGVG CCCCCCHHHHHHCCE	31.51	-
615	Acetylation	GDHPITAKAIAKGVG CCCCCCHHHHHHCCE	31.51	42499149
619	Ubiquitination	ITAKAIAKGVGIISE CCHHHHHHCCEEEEC	48.27	-
625	Phosphorylation	AKGVGIISEGNETVE HHCCEEEECCCCCHH	37.21	25403869
630	Phosphorylation	IISEGNETVEDIAAR EEECCCCCHHHHHHH	32.94	28551015
643	Phosphorylation	ARLNIPVSQVNPRDA HHCCCCHHHCCHHHC	24.05	27097102
651	Ubiquitination	QVNPRDAKACVIHGT HCCHHHCCEEEEECC	46.75	-
661	Ubiquitination	VIHGTDLKDFTSEQI EEECCCHHHCCHHHH	54.31	-
665	Phosphorylation	TDLKDFTSEQIDEIL CCHHHCCHHHHHHHH	27.74	-
688	Ubiquitination	ARTSPQQKLIIVEGC EECCCCCEEEEEECC	36.08	-
695	S-nitrosocysteine	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	-
695	S-nitrosylation	KLIIVEGCQRQGAIV EEEEEECCCCCCCEE	1.58	16418269
712	Phosphorylation	TGDGVNDSPALKKAD ECCCCCCCHHHHHCC	13.58	30240740
716	"N6,N6-dimethyllysine"	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	-
716	Ubiquitination	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	-
716	Acetylation	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	4199085
716	Methylation	VNDSPALKKADIGVA CCCCHHHHHCCCEEE	48.28	-
717	"N6,N6-dimethyllysine"	NDSPALKKADIGVAM CCCHHHHHCCCEEEC	52.74	-
717	Acetylation	NDSPALKKADIGVAM CCCHHHHHCCCEEEC	52.74	4199083
717	Ubiquitination	NDSPALKKADIGVAM CCCHHHHHCCCEEEC	52.74	-
717	Methylation	NDSPALKKADIGVAM CCCHHHHHCCCEEEC	52.74	-
763	"N6,N6-dimethyllysine"	RLIFDNLKKSIAYTL EEEECCHHHHHHHHH	51.21	-
763	Methylation	RLIFDNLKKSIAYTL EEEECCHHHHHHHHH	51.21	-
763	Ubiquitination	RLIFDNLKKSIAYTL EEEECCHHHHHHHHH	51.21	-
764	"N6,N6-dimethyllysine"	LIFDNLKKSIAYTLT EEECCHHHHHHHHHH	50.70	-
764	Ubiquitination	LIFDNLKKSIAYTLT EEECCHHHHHHHHHH	50.70	-
764	Methylation	LIFDNLKKSIAYTLT EEECCHHHHHHHHHH	50.70	-
819	Phosphorylation	LAYEAAESDIMKRQP HHHHHHHHHHHHCCC	27.92	-
833	Ubiquitination	PRNPRTDKLVNERLI CCCCCCHHHHHHHHH	54.44	-
833	Acetylation	PRNPRTDKLVNERLI CCCCCCHHHHHHHHH	54.44	72585921
933	Phosphorylation	ICKTRRNSVFQQGMK EECCCCCHHHHHHCC	23.61	25403869
940	Ubiquitination	SVFQQGMKNKILIFG HHHHHHCCCCEEEEE	63.01	-
1009	Ubiquitination	NPGGWVEKETYY--- CCCCCCEEECCC---	45.82	-
1009	Acetylation	NPGGWVEKETYY--- CCCCCCEEECCC---	45.82	22902405
1012	Phosphorylation	GWVEKETYY------ CCCEEECCC------	14.31	-
1013	Phosphorylation	WVEKETYY------- CCEEECCC-------	21.35	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
933	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AT1A3_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AT1A3_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ITPR1_RAT	Itpr1	physical	14593108
NAC1_RAT	Slc8a1	physical	14593108
SPTN1_RAT	Sptan1	physical	14593108

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.