ASM_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ASM_HUMAN
• UniProt AC: P17405
• Protein Name: Sphingomyelin phosphodiesterase
• Gene Name: SMPD1
• Organism: Homo sapiens (Human).
• Sequence Length: 629
• Subcellular Localization: Lysosome . Secreted .
• Protein Description: Converts sphingomyelin to ceramide. [PubMed: 1840600]
• Protein Sequence: MPRYGASLRQSCPRSGREQGQDGTAGAPGLLWMGLVLALALALALALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPYPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLAAQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETYILNLTQANIPGAIPHWQLLYRARETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPDGSLPEAQSLWPRPLFC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MPRYGASLRQSCPR -CCCCCCCHHHHCCC	21.52	30631047
86	N-linked_Glycosylation	RDVFGWGNLTCPICK HHHHCCCCCCCHHHH	26.18	27349982
88	N-linked_Glycosylation	VFGWGNLTCPICKGL HHCCCCCCCHHHHHH	21.89	27349982
88	N-linked_Glycosylation	VFGWGNLTCPICKGL HHCCCCCCCHHHHHH	21.89	27349982
114	Phosphorylation	PNVARVGSVAIKLCN CCCHHHHHHHHHHHH	12.91	17322306
116	Phosphorylation	VARVGSVAIKLCNLL CHHHHHHHHHHHHHH	8.56	17322306
120	S-nitrosylation	GSVAIKLCNLLKIAP HHHHHHHHHHHHHCC	2.61	19483679
120	S-nitrosocysteine	GSVAIKLCNLLKIAP HHHHHHHHHHHHHCC	2.61	-
175	N-linked_Glycosylation	WDIFSSWNISLPTVP HHHHHCCEECCCCCC	18.87	27349982
177	N-linked_Glycosylation	IFSSWNISLPTVPKP HHHCCEECCCCCCCC	25.69	27349982
231	Phosphorylation	PLCCRRGSGLPPASR CCHHCCCCCCCCCCC	35.03	27251275
237	Phosphorylation	GSGLPPASRPGAGYW CCCCCCCCCCCCCCC	44.79	27251275
239	Phosphorylation	GLPPASRPGAGYWGE CCCCCCCCCCCCCCC	34.19	27251275
335	N-linked_Glycosylation	PPPFIEGNHSSRWLY CCCCCCCCCCHHHHH	21.05	27349982
337	N-linked_Glycosylation	PFIEGNHSSRWLYEA CCCCCCCCHHHHHHH	26.12	27349982
337	N-linked_Glycosylation	PFIEGNHSSRWLYEA CCCCCCCCHHHHHHH	26.12	27349982
395	N-linked_Glycosylation	ENFWLLINSTDPAGQ CCEEEEEECCCCHHH	38.53	27349982
397	N-linked_Glycosylation	FWLLINSTDPAGQLQ EEEEEECCCCHHHHH	41.32	27349982
503	N-linked_Glycosylation	RVYQIDGNYSGSSHV EEEEECCCCCCCEEE	24.85	27349982
505	N-linked_Glycosylation	YQIDGNYSGSSHVVL EEECCCCCCCEEEEE	35.88	27349982
508	Phosphorylation	DGNYSGSSHVVLDHE CCCCCCCEEEEECCC	24.67	17698617
510	Phosphorylation	NYSGSSHVVLDHETY CCCCCEEEEECCCEE	5.03	17303575
520	N-linked_Glycosylation	DHETYILNLTQANIP CCCEEEEEHHHCCCC	31.33	27349982
522	N-linked_Glycosylation	ETYILNLTQANIPGA CEEEEEHHHCCCCCC	25.27	27349982

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
510	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
510	S	Phosphorylation	Phosphorylated at Ser-510 by PRKCD upon stress stimuli.	20807762

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ASM_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
OS9_HUMAN	OS9	physical	26186194
AL3A2_HUMAN	ALDH3A2	physical	26186194
ALG11_HUMAN	ALG11	physical	26186194
S27A2_HUMAN	SLC27A2	physical	26186194
CALX_HUMAN	CANX	physical	28514442
SE1L1_HUMAN	SEL1L	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 257200: Niemann-Pick disease A (NPDA)
• 607616: Niemann-Pick disease B (NPDB)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Functional characterization of the N-glycosylation sites of humanacid sphingomyelinase by site-directed mutagenesis.";
Ferlinz K., Hurwitz R., Moczall H., Lansmann S., Schuchman E.H.,Sandhoff K.;
Eur. J. Biochem. 243:511-517(1997).
Cited for: GLYCOSYLATION AT ASN-86; ASN-175; ASN-335; ASN-395 AND ASN-520.
PubMed: 9030779