ASB4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ASB4_MOUSE
• UniProt AC: Q9WV71
• Protein Name: Ankyrin repeat and SOCS box protein 4
• Gene Name: Asb4
• Organism: Mus musculus (Mouse).
• Sequence Length: 426
• Protein Description: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism..
• Protein Sequence: MDGITAPISKAGAAKLVKKDFLEALKTNDFGKLKAILIERQIDVDTVFEVEDENMILASYKQGYWLPSYKLKSSWATGLHLSVLLGHVECLMVLLDHNATINCRPNGKTPLHVACEIANLECVKILCDRGAKLNCYSLSGHTALHFCTTPSSILCAKQLVLRGANVNMKTNNQDEETPLHTAAHFGLSELVAFYVENGAIVDSMNAHMETPLAIATYWALRFKEQEYSREHHLICRTLLDNNAEVNARDDDFKSPLHKAAWNCDHVLMHMMLEAGAEANLMDINGCAAIQYVLKVTSVRPAAQPEICYQLLLNHGAARIYPPQFHKVIQACHSCPKAIEVVVNAYEHIRWNIKWRRAIPDDDLEKHWDFYHSLFKVCCNTPRTLMHLSRCAIRKALHNRCHKAIPMLSLPLPLKKYLLLEPEGIIY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
64	Phosphorylation	LASYKQGYWLPSYKL EEEEECCEECCCEEC	10.98	29514104
246	Hydroxylation	LDNNAEVNARDDDFK HHCCCCCCCCCCCCC	22.29	17636018
426	Phosphorylation	LEPEGIIY------- HCCCCCCC-------	15.58	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ASB4_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
246	N	Hydroxylation	Hydroxylation at Asn-246 by HIF1AN may provide an oxygen-dependent regulation mechanism for the function of ASB4 in promoting vascular differentiation.	17636018

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ASB4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IRS4_MOUSE	Irs4	physical	21955513
IRS4_HUMAN	IRS4	physical	21955513

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Hydroxylation

"ASB4 is a hydroxylation substrate of FIH and promotes vasculardifferentiation via an oxygen-dependent mechanism.";
Ferguson J.E. III, Wu Y., Smith K., Charles P., Powers K., Wang H.,Patterson C.;
Mol. Cell. Biol. 27:6407-6419(2007).
Cited for: PROTEIN SEQUENCE OF 237-248, FUNCTION, TISSUE SPECIFICITY,DEVELOPMENTAL STAGE, INTERACTION WITH TCEB2; CUL5; RBX1; CUL2 ANDHIF1AN, HYDROXYLATION AT ASN-246, AND MUTAGENESIS OF LEU-238; LEU-239;GLU-244; 244-GLU--ASN-246; VAL-245; ASN-246 AND LEU-256.
PubMed: 17636018