AKAP4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AKAP4_HUMAN
• UniProt AC: Q5JQC9
• Protein Name: A-kinase anchor protein 4
• Gene Name: AKAP4 {ECO:0000312|HGNC:HGNC:374}
• Organism: Homo sapiens (Human).
• Sequence Length: 854
• Subcellular Localization: Cell projection, cilium, flagellum . Localizes to the principle piece of the sperm flagellum.
• Protein Description: Major structural component of sperm fibrous sheath. Plays a role in sperm motility..
• Protein Sequence: MMAYSDTTMMSDDIDWLRSHRGVCKVDLYNPEGQQDQDRKVICFVDVSTLNVEDKDYKDAASSSSEGNLNLGSLEEKEIIVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQHALSPSTSTCKHKVGDTEGEYHRASSENCYSVYADQVNIDYLMNRPQNLRLEMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDGECSIDDLSFYVNRLSSLVIQMAHKEIKEKLEGKSKCLHHSICPSPGNKERISPRTPASKIASEMAYEAVELTAAEMRGTGEESREGGQKSFLYSELSNKSKSGDKQMSQRESKEFADSISKGLMVYANQVASDMMVSLMKTLKVHSSGKPIPASVVLKRVLLRHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNQWKQNATDIMEAMLKRLVSALIGEEKETKSQSLSYASLKAGSHDPKCRNQSLEFSTMKAEMKERDKGKMKSDPCKSLTSAEKVGEHILKEGLTIWNQKQGNSCKVATKACSNKDEKGEKINASTDSLAKDLIVSALKLIQYHLTQQTKGKDTCEEDCPGSTMGYMAQSTQYEKCGGGQSAKALSVKQLESHRAPGPSTCQKENQHLDSQKMDMSNIVLMLIQKLLNENPFKCEDPCEGENKCSEPRASKAASMSNRSDKAEEQCQEHQELDCTSGMKQANGQFIDKLVESVMKLCLIMAKYSNDGAALAELEEQAASANKPNFRGTRCIHSGAMPQNYQDSLGHEVIVNNQCSTNSLQKQLQAVLQWIAASQFNVPMLYFMGDKDGQLEKLPQVSAKAAEKGYSVGGLLQEVMKFAKERQPDEAVGKVARKQLLDWLLANL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MMAYSDTTMMS ----CCCCCCCCCCC	6.81	20068231
5	Phosphorylation	---MMAYSDTTMMSD ---CCCCCCCCCCCC	20.95	20068231
7	Phosphorylation	-MMAYSDTTMMSDDI -CCCCCCCCCCCCCH	15.62	20068231
8	Phosphorylation	MMAYSDTTMMSDDID CCCCCCCCCCCCCHH	18.83	20068231
11	Phosphorylation	YSDTTMMSDDIDWLR CCCCCCCCCCHHHHH	22.70	20068231
19	Phosphorylation	DDIDWLRSHRGVCKV CCHHHHHHCCCEEEE	18.50	20068231
29	Phosphorylation	GVCKVDLYNPEGQQD CEEEEECCCCCCCCC	24.98	30622161
48	Phosphorylation	VICFVDVSTLNVEDK EEEEEECCCCCCCCC	23.78	30622161
49	Phosphorylation	ICFVDVSTLNVEDKD EEEEECCCCCCCCCC	23.38	30622161
57	Phosphorylation	LNVEDKDYKDAASSS CCCCCCCHHHHHCCC	19.26	46161413
58	Acetylation	NVEDKDYKDAASSSS CCCCCCHHHHHCCCC	51.53	25038526
62	Phosphorylation	KDYKDAASSSSEGNL CCHHHHHCCCCCCCC	32.26	22817900
63	Phosphorylation	DYKDAASSSSEGNLN CHHHHHCCCCCCCCC	32.91	22817900
64	Phosphorylation	YKDAASSSSEGNLNL HHHHHCCCCCCCCCC	29.88	22817900
65	Phosphorylation	KDAASSSSEGNLNLG HHHHCCCCCCCCCCC	52.01	22817900
73	Phosphorylation	EGNLNLGSLEEKEII CCCCCCCCCCCCEEE	35.46	30622161
91	Phosphorylation	DTEKKDQSKTEGSVC CCCCCCCCCCCCCEE	52.24	30622161
93	Phosphorylation	EKKDQSKTEGSVCLF CCCCCCCCCCCEEEE	51.31	30622161
96	Phosphorylation	DQSKTEGSVCLFKQA CCCCCCCCEEEEECC	11.92	30622161
101	Acetylation	EGSVCLFKQAPSDPV CCCEEEEECCCCCHH	32.38	25038526
105	Phosphorylation	CLFKQAPSDPVSVLN EEEECCCCCHHHHHH	58.52	30622161
109	Phosphorylation	QAPSDPVSVLNWLLS CCCCCHHHHHHHHHH	26.69	30622161
116	Phosphorylation	SVLNWLLSDLQKYAL HHHHHHHHHHHHHHH	33.82	30622161
121	Phosphorylation	LLSDLQKYALGFQHA HHHHHHHHHHHCCCC	8.29	30622161
130	Phosphorylation	LGFQHALSPSTSTCK HHCCCCCCCCCCCCC	20.00	30622161
132	Phosphorylation	FQHALSPSTSTCKHK CCCCCCCCCCCCCCC	31.45	30622161
133	Phosphorylation	QHALSPSTSTCKHKV CCCCCCCCCCCCCCC	31.29	30622161
134	Phosphorylation	HALSPSTSTCKHKVG CCCCCCCCCCCCCCC	35.82	30622161
135	Phosphorylation	ALSPSTSTCKHKVGD CCCCCCCCCCCCCCC	26.18	30622161
143	Phosphorylation	CKHKVGDTEGEYHRA CCCCCCCCCCCEEEC	39.70	25693802
147	Phosphorylation	VGDTEGEYHRASSEN CCCCCCCEEECCCCC	13.89	30622161
151	Phosphorylation	EGEYHRASSENCYSV CCCEEECCCCCCEEE	37.83	22817900
152	Phosphorylation	GEYHRASSENCYSVY CCEEECCCCCCEEEE	31.66	30622161
156	Phosphorylation	RASSENCYSVYADQV ECCCCCCEEEECCCC	16.86	25693802
157	Phosphorylation	ASSENCYSVYADQVN CCCCCCEEEECCCCC	15.73	30622161
159	Phosphorylation	SENCYSVYADQVNID CCCCEEEECCCCCHH	9.87	25693802
190	Phosphorylation	KNTNNNQSPSAPPAK CCCCCCCCCCCCCCC	23.97	30622161
192	Phosphorylation	TNNNQSPSAPPAKPP CCCCCCCCCCCCCCC	59.67	30622161
200	Phosphorylation	APPAKPPSTQRAVIS CCCCCCCCCCCEEEC	45.78	30622161
201	Phosphorylation	PPAKPPSTQRAVISP CCCCCCCCCCEEECC	28.04	30622161
207	Phosphorylation	STQRAVISPDGECSI CCCCEEECCCCCCCH	15.44	30622161
213	Phosphorylation	ISPDGECSIDDLSFY ECCCCCCCHHHHHHH	25.42	30622161
218	Phosphorylation	ECSIDDLSFYVNRLS CCCHHHHHHHHHHHH	22.86	30622161
220	Phosphorylation	SIDDLSFYVNRLSSL CHHHHHHHHHHHHHH	8.08	30622161
225	Phosphorylation	SFYVNRLSSLVIQMA HHHHHHHHHHHHHHH	20.56	30622161
226	Phosphorylation	FYVNRLSSLVIQMAH HHHHHHHHHHHHHHH	31.11	30622161
234	Acetylation	LVIQMAHKEIKEKLE HHHHHHHHHHHHHHC	52.15	25038526
250	Phosphorylation	KSKCLHHSICPSPGN CCCCCCEECCCCCCC	18.35	46161407
254	Phosphorylation	LHHSICPSPGNKERI CCEECCCCCCCHHCC	40.82	30622161
262	Phosphorylation	PGNKERISPRTPASK CCCHHCCCCCCCHHH	17.98	25072903
265	Phosphorylation	KERISPRTPASKIAS HHCCCCCCCHHHHHH	26.96	22817900
268	Phosphorylation	ISPRTPASKIASEMA CCCCCCHHHHHHHHH	26.29	22817900
272	Phosphorylation	TPASKIASEMAYEAV CCHHHHHHHHHHHHH	31.23	22817900
276	Phosphorylation	KIASEMAYEAVELTA HHHHHHHHHHHHHHH	11.78	30622161
282	Phosphorylation	AYEAVELTAAEMRGT HHHHHHHHHHHHCCC	15.43	30622161
289	Phosphorylation	TAAEMRGTGEESREG HHHHHCCCCCCCCCC	30.93	68704795
293	Phosphorylation	MRGTGEESREGGQKS HCCCCCCCCCCCHHH	31.18	22817900
300	Phosphorylation	SREGGQKSFLYSELS CCCCCHHHHHHHHHC	16.76	22817900
303	Phosphorylation	GGQKSFLYSELSNKS CCHHHHHHHHHCCCC	9.70	22817900
304	Phosphorylation	GQKSFLYSELSNKSK CHHHHHHHHHCCCCC	33.92	22817900
307	Phosphorylation	SFLYSELSNKSKSGD HHHHHHHCCCCCCCC	37.85	68704801
310	Phosphorylation	YSELSNKSKSGDKQM HHHHCCCCCCCCHHH	35.98	30622161
312	Phosphorylation	ELSNKSKSGDKQMSQ HHCCCCCCCCHHHHH	60.35	30622161
318	Phosphorylation	KSGDKQMSQRESKEF CCCCHHHHHHHHHHH	24.48	101680507
322	Phosphorylation	KQMSQRESKEFADSI HHHHHHHHHHHHHHH	39.41	30622161
328	Phosphorylation	ESKEFADSISKGLMV HHHHHHHHHHHHHHH	25.90	23403867
330	Phosphorylation	KEFADSISKGLMVYA HHHHHHHHHHHHHHH	25.31	23403867
336	Phosphorylation	ISKGLMVYANQVASD HHHHHHHHHHHHHHH	5.95	25693802
342	Phosphorylation	VYANQVASDMMVSLM HHHHHHHHHHHHHHH	27.47	30622161
347	Phosphorylation	VASDMMVSLMKTLKV HHHHHHHHHHHHHCC	12.59	30622161
356	Phosphorylation	MKTLKVHSSGKPIPA HHHHCCCCCCCCCCH	43.72	30622161
357	Phosphorylation	KTLKVHSSGKPIPAS HHHCCCCCCCCCCHH	35.64	30622161
364	Phosphorylation	SGKPIPASVVLKRVL CCCCCCHHHHHHHHH	13.85	36013029
376	Acetylation	RVLLRHTKEIVSDLI HHHHHHHHHHHHHHH	39.04	25038526
380	Phosphorylation	RHTKEIVSDLIDSCM HHHHHHHHHHHHHHH	32.15	30622161
399	Phosphorylation	NITGVLMTDSDFVSA HCCCEECCCHHHHHH	28.34	30622161
401	Phosphorylation	TGVLMTDSDFVSAVK CCEECCCHHHHHHHH	24.63	30622161
405	Phosphorylation	MTDSDFVSAVKRNLF CCCHHHHHHHHHHHH	27.60	30622161
420	Phosphorylation	NQWKQNATDIMEAML HHHHHCHHHHHHHHH	33.34	20068231
428	Acetylation	DIMEAMLKRLVSALI HHHHHHHHHHHHHHH	30.51	25038526
432	Phosphorylation	AMLKRLVSALIGEEK HHHHHHHHHHHCCCC	23.19	20068231
439	Acetylation	SALIGEEKETKSQSL HHHHCCCCCCCCCCC	67.40	25038526
441	Phosphorylation	LIGEEKETKSQSLSY HHCCCCCCCCCCCCH	47.25	20068231
442	Acetylation	IGEEKETKSQSLSYA HCCCCCCCCCCCCHH	47.81	25038526
443	Phosphorylation	GEEKETKSQSLSYAS CCCCCCCCCCCCHHH	32.21	30622161
445	Phosphorylation	EKETKSQSLSYASLK CCCCCCCCCCHHHHH	26.77	30622161
447	Phosphorylation	ETKSQSLSYASLKAG CCCCCCCCHHHHHCC	24.73	22817900
448	Phosphorylation	TKSQSLSYASLKAGS CCCCCCCHHHHHCCC	12.90	22817900
450	Phosphorylation	SQSLSYASLKAGSHD CCCCCHHHHHCCCCC	23.36	22817900
464	Phosphorylation	DPKCRNQSLEFSTMK CHHHCCCCCCHHHHH	33.15	25693802
468	Phosphorylation	RNQSLEFSTMKAEMK CCCCCCHHHHHHHHH	20.44	30622161
469	Phosphorylation	NQSLEFSTMKAEMKE CCCCCHHHHHHHHHH	28.19	30622161
471	Acetylation	SLEFSTMKAEMKERD CCCHHHHHHHHHHHH	41.08	25038526
484	Phosphorylation	RDKGKMKSDPCKSLT HHCCCCCCCCCCCCC	43.20	25693802
489	Phosphorylation	MKSDPCKSLTSAEKV CCCCCCCCCCCHHHH	43.53	25693802
491	Phosphorylation	SDPCKSLTSAEKVGE CCCCCCCCCHHHHHH	32.74	25693802
492	Phosphorylation	DPCKSLTSAEKVGEH CCCCCCCCHHHHHHH	39.46	50423
502	Acetylation	KVGEHILKEGLTIWN HHHHHHHHHCCEEEE	49.48	25038526
506	Phosphorylation	HILKEGLTIWNQKQG HHHHHCCEEEECCCC	34.50	30622161
536	Phosphorylation	KGEKINASTDSLAKD HCCCCCCCHHHHHHH	28.41	22817900
537	Phosphorylation	GEKINASTDSLAKDL CCCCCCCHHHHHHHH	27.48	30622161
539	Phosphorylation	KINASTDSLAKDLIV CCCCCHHHHHHHHHH	30.25	22817900
547	Phosphorylation	LAKDLIVSALKLIQY HHHHHHHHHHHHHHH	22.38	30622161
550	Acetylation	DLIVSALKLIQYHLT HHHHHHHHHHHHHHH	43.17	25038526
554	Phosphorylation	SALKLIQYHLTQQTK HHHHHHHHHHHCCCC	7.57	113136191
557	Phosphorylation	KLIQYHLTQQTKGKD HHHHHHHHCCCCCCC	12.81	113136199
565	Phosphorylation	QQTKGKDTCEEDCPG CCCCCCCCCCCCCCC	25.88	68737929
573	Phosphorylation	CEEDCPGSTMGYMAQ CCCCCCCCHHHHHHC	10.60	68737935
574	Phosphorylation	EEDCPGSTMGYMAQS CCCCCCCHHHHHHCC	22.11	25693802
577	Phosphorylation	CPGSTMGYMAQSTQY CCCCHHHHHHCCCCC	4.39	30622161
581	Phosphorylation	TMGYMAQSTQYEKCG HHHHHHCCCCCEECC	14.13	28985074
582	Phosphorylation	MGYMAQSTQYEKCGG HHHHHCCCCCEECCC	24.08	25693802
584	Phosphorylation	YMAQSTQYEKCGGGQ HHHCCCCCEECCCCH	19.90	30622161
597	Phosphorylation	GQSAKALSVKQLESH CHHCEEEEHHHHHHC	31.93	27174698
603	Phosphorylation	LSVKQLESHRAPGPS EEHHHHHHCCCCCCH	27.19	27174698
610	Phosphorylation	SHRAPGPSTCQKENQ HCCCCCCHHHHHHHC	47.97	30622161
611	Phosphorylation	HRAPGPSTCQKENQH CCCCCCHHHHHHHCC	23.30	30622161
621	Phosphorylation	KENQHLDSQKMDMSN HHHCCHHHCCCCHHH	37.75	30622161
623	Acetylation	NQHLDSQKMDMSNIV HCCHHHCCCCHHHHH	40.60	25038526
627	Phosphorylation	DSQKMDMSNIVLMLI HHCCCCHHHHHHHHH	21.09	30622161
644	Acetylation	LLNENPFKCEDPCEG HHHCCCCCCCCCCCC	37.66	25038526
656	Phosphorylation	CEGENKCSEPRASKA CCCCCCCCCCCHHHH	52.85	30622161
661	Phosphorylation	KCSEPRASKAASMSN CCCCCCHHHHHHHCC	24.89	30622161
665	Phosphorylation	PRASKAASMSNRSDK CCHHHHHHHCCCHHH	27.83	22817900
667	Phosphorylation	ASKAASMSNRSDKAE HHHHHHHCCCHHHHH	26.96	50439
670	Phosphorylation	AASMSNRSDKAEEQC HHHHCCCHHHHHHHH	47.95	30622161
672	Acetylation	SMSNRSDKAEEQCQE HHCCCHHHHHHHHHH	59.97	25038526
686	Phosphorylation	EHQELDCTSGMKQAN HHHHHHCCCCCHHHC	28.16	30622161
687	Phosphorylation	HQELDCTSGMKQANG HHHHHCCCCCHHHCC	43.13	30622161
699	Acetylation	ANGQFIDKLVESVMK HCCHHHHHHHHHHHH	50.01	25038526
703	Phosphorylation	FIDKLVESVMKLCLI HHHHHHHHHHHHHHH	21.74	30622161
713	Acetylation	KLCLIMAKYSNDGAA HHHHHHHHHCCCHHH	31.65	25038526
714	Phosphorylation	LCLIMAKYSNDGAAL HHHHHHHHCCCHHHH	11.81	30622161
715	Phosphorylation	CLIMAKYSNDGAALA HHHHHHHCCCHHHHH	27.95	30622161
730	Phosphorylation	ELEEQAASANKPNFR HHHHHHHHCCCCCCC	35.08	30622161
744	Phosphorylation	RGTRCIHSGAMPQNY CCCCCCCCCCCCCCC	14.34	30622161
751	Phosphorylation	SGAMPQNYQDSLGHE CCCCCCCCHHCCCCE	14.40	30622161
754	Phosphorylation	MPQNYQDSLGHEVIV CCCCCHHCCCCEEEE	22.26	30622161
766	Phosphorylation	VIVNNQCSTNSLQKQ EEECCCCCCHHHHHH	22.36	30622161
767	Phosphorylation	IVNNQCSTNSLQKQL EECCCCCCHHHHHHH	36.13	30622161
769	Phosphorylation	NNQCSTNSLQKQLQA CCCCCCHHHHHHHHH	32.06	30622161
784	Phosphorylation	VLQWIAASQFNVPML HHHHHHHHHCCCCEE	26.70	30622161
792	Phosphorylation	QFNVPMLYFMGDKDG HCCCCEEEEEECCCC	5.81	7452227
808	Phosphorylation	LEKLPQVSAKAAEKG CCCCCCCHHHHHHCC	20.85	20068231
816	Phosphorylation	AKAAEKGYSVGGLLQ HHHHHCCCCHHHHHH	15.96	25693802
817	Phosphorylation	KAAEKGYSVGGLLQE HHHHCCCCHHHHHHH	23.97	20068231
827	Acetylation	GLLQEVMKFAKERQP HHHHHHHHHHHHHCC	47.55	25038526

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
265	T	Phosphorylation	Kinase	MAPK1	P28482	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AKAP4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AKAP4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
FSIP1_HUMAN	FSIP1	physical	12606363
AKAP3_HUMAN	AKAP3	physical	12606363
KAP0_HUMAN	PRKAR1A	physical	12606363
KAP0_HUMAN	PRKAR1A	physical	9852104
KAP2_HUMAN	PRKAR2A	physical	9852104

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteome analysis of capacitated human sperm. Evidence oftyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.";
Ficarro S., Chertihin O., Westbrook V.A., White F., Jayes F.,Kalab P., Marto J.A., Shabanowitz J., Herr J.C., Hunt D.F.,Visconti P.E.;
J. Biol. Chem. 278:11579-11589(2003).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62; SER-63; SER-64;SER-65; SER-151; THR-265; SER-268; SER-272; SER-293; SER-300; TYR-303;SER-304; SER-447; TYR-448; SER-450; SER-492; SER-536; SER-539; SER-665AND SER-667, AND MASS SPECTROMETRY.
PubMed: 12509440