AFR1_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AFR1_YEAST
• UniProt AC: P33304
• Protein Name: Protein AFR1
• Gene Name: AFR1
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 620
• Protein Description: Acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation..
• Protein Sequence: MEGSYLSAQENQPIPERLIPRSNSTSNLFALSSTFSKLNVRNDADYNYSNPNKKRHIYSGEIDCRSVTAARKFPVRSCSMTAAQQRKRTALFTVRERNSYHEGFNNDQDYVSQYQKPQYTFGVYKELTPYQLQRSKMKRSFQFPNGEIYKPKLDGKCTHSLKKPELNSRDSSLFKFSEKKGRNLSKDFVGPHNGTSVIHIPPNDTGYGVNSLELNTSVPSTIKSSVSSTSPISAVNTLTSLPESQTDDDDGYENKTVTISYCFENTVNEKHGSHIEKLDLSTKEKTKPTTNSGLFDRKKKTILGTEKYRCIKSQSKLKLGSVLKKLWRTSGNSNTKHGKKDTKRRRIPIDDMVTHSDGNSEAENDIELMDANLDGIEFDDDETLMDTDSIFDDLLSKENDKYDLRRRQLEIRQKLHETSHNDDGKVSFRDTEKHNVNEGLIDKTIIEEFSKLGEYIIDTRNQPPPRSSKRPSLDDNESARYFYNISTDLRQSLSGPISLPMHVGNDMVNRLRNDWEYIRFEDRRNSLPDSSFDKVETPPKPIKKDVRFAKEVCLASTWSSNAYERANPEFIMNRHRLLWMMKVHPSMNSAMNEIKLELNSYKKNEMVVHENSKCFTHYLI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Phosphorylation	PERLIPRSNSTSNLF CCHHCCCCCCCCCEE	29.76	15665377
24	Phosphorylation	RLIPRSNSTSNLFAL HHCCCCCCCCCEEEE	34.16	17330950
25	Phosphorylation	LIPRSNSTSNLFALS HCCCCCCCCCEEEEE	26.36	19779198
26	Phosphorylation	IPRSNSTSNLFALSS CCCCCCCCCEEEEEH	31.21	29688323
66	Phosphorylation	SGEIDCRSVTAARKF ECCCCCCCCCHHCCC	30.10	30377154
79	Phosphorylation	KFPVRSCSMTAAQQR CCCCCCCCCCHHHHH	22.03	28889911
472	Phosphorylation	PRSSKRPSLDDNESA CCCCCCCCCCCCHHH	48.76	17330950
478	Phosphorylation	PSLDDNESARYFYNI CCCCCCHHHHHHHCC	25.05	28132839
526	Phosphorylation	RFEDRRNSLPDSSFD EEHHHCCCCCCCCCC	39.19	17330950
530	Phosphorylation	RRNSLPDSSFDKVET HCCCCCCCCCCCCCC	30.85	28132839
586	Phosphorylation	WMMKVHPSMNSAMNE HHHHHCHHHHHHHHH	18.73	27017623

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of AFR1_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AFR1_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AFR1_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDC12_YEAST	CDC12	physical	9201710
CDC12_YEAST	CDC12	physical	7823940
GCN5_YEAST	GCN5	genetic	7603436
ATG17_YEAST	ATG17	physical	18719252
PP12_YEAST	GLC7	physical	18552279
CDC10_YEAST	CDC10	physical	18552279

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of alpha-factor-arrestedSaccharomyces cerevisiae.";
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,Elias J.E., Gygi S.P.;
J. Proteome Res. 6:1190-1197(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472 AND SER-526, ANDMASS SPECTROMETRY.
PubMed: 17330950

"Quantitative phosphoproteomics applied to the yeast pheromonesignaling pathway.";
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,Mann M., Jensen O.N.;
Mol. Cell. Proteomics 4:310-327(2005).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, AND MASSSPECTROMETRY.
PubMed: 15665377