WWOX_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WWOX_MOUSE
• UniProt AC: Q91WL8
• Protein Name: WW domain-containing oxidoreductase
• Gene Name: Wwox
• Organism: Mus musculus (Mouse).
• Sequence Length: 414
• Subcellular Localization: Cytoplasm . Nucleus . Mitochondrion . Partially localizes to the mitochondria (By similarity). Translocates to the nucleus in response to TGFB1 (PubMed:19366691). Translocates to the nucleus upon genotoxic stress or TNF stimulation (PubMed:11058590).
• Protein Description: Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm (By similarity). May also play a role in tumor necrosis factor (TNF)-mediated cell death. Required for normal bone development..
• Protein Sequence: MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVLVTGANSGIGFETAKSFALHGAHVILACRNLSRASEAVSRILEEWHKAKVEAMTLDLAVLRSVQHFAEAFKAKNVSLHVLVCNAGTFALPWGLTKDGLETTFQVNHLGHFYLVQLLQDVLCRSSPARVIVVSSESHRFTDINDSSGKLDLSRLSPPRSDYWAMLAYNRSKLCNILFSNELHRRLSPRGVTSNAVHPGNMMYSAIHRNSWVYKLLFTLARPFTKSMQQGAATTVYCAVAPELEGLGGMYFNNCCRCLPSEEAQSEETARALWELSERLIQDRLGSPSS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MAALRYAGLDDTD --CCCCCCCCCCCCC	13.08	27087446
12	Phosphorylation	RYAGLDDTDSEDELP CCCCCCCCCCCCCCC	40.88	25521595
14	Phosphorylation	AGLDDTDSEDELPPG CCCCCCCCCCCCCCC	49.38	25521595
33	Phosphorylation	TTKDGWVYYANHTEE ECCCEEEEEEECCCC	7.14	22817900
278	Phosphorylation	SSGKLDLSRLSPPRS CCCCCCHHHCCCCCH	30.73	-
281	Phosphorylation	KLDLSRLSPPRSDYW CCCHHHCCCCCHHHH	31.57	24719451
287	Phosphorylation	LSPPRSDYWAMLAYN CCCCCHHHHHHHHHC	9.05	16288044
411	Phosphorylation	LIQDRLGSPSS---- HHHHHHCCCCC----	26.63	21454597
413	Phosphorylation	QDRLGSPSS------ HHHHCCCCC------	50.83	24759943
414	Phosphorylation	DRLGSPSS------- HHHCCCCC-------	47.85	24759943

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
287	Y	Phosphorylation	Kinase	TNK2	O54967	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WWOX_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WWOX_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RUNX2_MOUSE	Runx2	physical	18487609
P53_MOUSE	Trp53	physical	15580310

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphoproteomic analysis of the developing mouse brain.";
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
Mol. Cell. Proteomics 3:1093-1101(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, AND MASSSPECTROMETRY.
PubMed: 15345747

"Transforming growth factor beta1 signaling via interaction with cellsurface Hyal-2 and recruitment of WWOX/WOX1.";
Hsu L.-J., Schultz L., Hong Q., Van Moer K., Heath J., Li M.-Y.,Lai F.-J., Lin S.-R., Lee M.-H., Lo C.-P., Lin Y.-S., Chen S.-T.,Chang N.-S.;
J. Biol. Chem. 284:16049-16059(2009).
Cited for: FUNCTION, PHOSPHORYLATION AT TYR-33, SUBCELLULAR LOCATION, ANDINTERACTION WITH HYAL2.
PubMed: 19366691