dbPTM

ULK1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ULK1_MOUSE
UniProt AC	O70405
Protein Name	Serine/threonine-protein kinase ULK1
Gene Name	Ulk1
Organism	Mus musculus (Mouse).
Sequence Length	1051
Subcellular Localization	Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome
Protein Description	Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. [PubMed: 25040165]
Protein Sequence	MEPGRGGVETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHTMRTLSEDTVRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPGGRRANPSNIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPAIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASTPIKKSPPVPVPSYPSSGSGSSSSSSSASHLASPPSLGEMPQLQKTLTSPADAAGFLQGSRDSGGSSKDSCDTDDFVMVPAQFPGDLVAEAASAKPPPDSLLCSGSSLVASAGLESHGRTPSPSPTCSSSPSPSGRPGPFSSNRYGASVPIPVPTQVHNYQRIEQNLQSPTQQQTARSSAIRRSGSTTPLGFGRASPSPPSHTDGAMLARKLSLGGGRPYTPSPQVGTIPERPSWSRVPSPQGADVRVGRSPRPGSSVPEHSPRTTGLGCRLHSAPNLSDFHVVRPKLPKPPTDPLGATFSPPQTSAPQPCPGLQSCRPLRGSPKLPDFLQRSPLPPILGSPTKAGPSFDFPKTPSSQNLLTLLARQGVVMTPPRNRTLPDLSEASPFHGQQLGSGLRPAEDTRGPFGRSFSTSRITDLLLKAAFGTQASDSGSTDSLQEKPMEIAPSAGFGGTLHPGARGGGASSPAPVVFTVGSPPSGATPPQSTRTRMFSVGSSSSLGSTGSSSARHLVPGACGEAPELSAPGHCCSLADPLAANLEGAVTFEAPDLPEETLMEQEHTETLHSLRFTLAFAQQVLEIAALKGSASEAAGGPEYQLQESVVADQISQLSREWGFAEQLVLYLKVAELLSSGLQTAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDGIHGVTAERLILSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHTLTDQADIENIAKCKLCIERRLSALLSGVYA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
87	Phosphorylation	DFQEMANSVYLVMEY EHHHHHHHHHHHHHH	11.55	19807128
147	Phosphorylation	KPQNILLSNPGGRRA CHHHEEECCCCCCCC	37.22	20139300
162	Acetylation	NPSNIRVKIADFGFA CCCCCEEEECHHHHH	23.48	22539723
180	Phosphorylation	QSNMMAATLCGSPMY HHCCHHHHHCCCCCC	17.03	21819378
195	Phosphorylation	MAPEVIMSQHYDGKA CCCHHHHCCCCCCCC	11.59	19807128
224	Phosphorylation	GKAPFQASSPQDLRL CCCCCCCCCHHHHHH	31.59	29472430
225	Phosphorylation	KAPFQASSPQDLRLF CCCCCCCCHHHHHHH	29.60	29472430
281	Phosphorylation	HHPFLDASTPIKKSP CCCCCCCCCCCCCCC	34.09	25521595
282	Phosphorylation	HPFLDASTPIKKSPP CCCCCCCCCCCCCCC	30.65	-
317	Phosphorylation	SHLASPPSLGEMPQL HHHCCCCCHHCCCHH	53.01	21258367
327	Phosphorylation	EMPQLQKTLTSPADA CCCHHHHHCCCHHHH	23.47	29899451
341	Phosphorylation	AAGFLQGSRDSGGSS HHHHHCCCCCCCCCC	21.69	19807128
347	Phosphorylation	GSRDSGGSSKDSCDT CCCCCCCCCCCCCCC	37.43	30352176
348	Phosphorylation	SRDSGGSSKDSCDTD CCCCCCCCCCCCCCC	43.70	30352176
403	Phosphorylation	ESHGRTPSPSPTCSS HHCCCCCCCCCCCCC	37.08	23684622
405	Phosphorylation	HGRTPSPSPTCSSSP CCCCCCCCCCCCCCC	36.86	23684622
410	Phosphorylation	SPSPTCSSSPSPSGR CCCCCCCCCCCCCCC	48.71	25338131
450	Phosphorylation	RIEQNLQSPTQQQTA HHHHHCCCHHHHHHH	32.83	25521595
452	Phosphorylation	EQNLQSPTQQQTARS HHHCCCHHHHHHHHH	44.23	25619855
456	Phosphorylation	QSPTQQQTARSSAIR CCHHHHHHHHHHHHH	21.50	25619855
459	Phosphorylation	TQQQTARSSAIRRSG HHHHHHHHHHHHHCC	22.97	20531401
460	Phosphorylation	QQQTARSSAIRRSGS HHHHHHHHHHHHCCC	23.35	20531401
465	Phosphorylation	RSSAIRRSGSTTPLG HHHHHHHCCCCCCCC	27.52	21659605
467	Phosphorylation	SAIRRSGSTTPLGFG HHHHHCCCCCCCCCC	30.29	21819378
468	Phosphorylation	AIRRSGSTTPLGFGR HHHHCCCCCCCCCCC	35.64	21659605
469	Phosphorylation	IRRSGSTTPLGFGRA HHHCCCCCCCCCCCC	20.43	21659605
477	Phosphorylation	PLGFGRASPSPPSHT CCCCCCCCCCCCCCC	25.26	27180971
479	Phosphorylation	GFGRASPSPPSHTDG CCCCCCCCCCCCCCH	47.12	27180971
482	Phosphorylation	RASPSPPSHTDGAML CCCCCCCCCCCHHHH	42.26	29472430
484	Phosphorylation	SPSPPSHTDGAMLAR CCCCCCCCCHHHHHH	40.23	29472430
494	Phosphorylation	AMLARKLSLGGGRPY HHHHHHHHCCCCCCC	28.18	24719451
502	Phosphorylation	LGGGRPYTPSPQVGT CCCCCCCCCCCCCCC	21.70	29899451
504	Phosphorylation	GGRPYTPSPQVGTIP CCCCCCCCCCCCCCC	21.84	-
521	Phosphorylation	PSWSRVPSPQGADVR CCCCCCCCCCCCCCE	27.31	23684622
532	Phosphorylation	ADVRVGRSPRPGSSV CCCEECCCCCCCCCC	21.23	23737553
537	Phosphorylation	GRSPRPGSSVPEHSP CCCCCCCCCCCCCCC	30.74	26060331
538	Phosphorylation	RSPRPGSSVPEHSPR CCCCCCCCCCCCCCC	47.83	24899341
543	Phosphorylation	GSSVPEHSPRTTGLG CCCCCCCCCCCCCCC	17.89	29895711
555	Phosphorylation	GLGCRLHSAPNLSDF CCCCCCCCCCCCCCC	50.44	25521595
560	Phosphorylation	LHSAPNLSDFHVVRP CCCCCCCCCCEEECC	44.89	18846507
574	Phosphorylation	PKLPKPPTDPLGATF CCCCCCCCCCCCCCC	59.79	21205641
597	Phosphorylation	QPCPGLQSCRPLRGS CCCCCCCCCCCCCCC	19.69	26060331
604	Phosphorylation	SCRPLRGSPKLPDFL CCCCCCCCCCCCHHH	16.66	26060331
606	Acetylation	RPLRGSPKLPDFLQR CCCCCCCCCCHHHHH	74.48	22539723
614	Phosphorylation	LPDFLQRSPLPPILG CCHHHHHCCCCCCCC	20.16	26824392
622	Phosphorylation	PLPPILGSPTKAGPS CCCCCCCCCCCCCCC	26.42	25521595
624	Phosphorylation	PPILGSPTKAGPSFD CCCCCCCCCCCCCCC	34.91	22942356
629	Phosphorylation	SPTKAGPSFDFPKTP CCCCCCCCCCCCCCC	36.21	28066266
635	Phosphorylation	PSFDFPKTPSSQNLL CCCCCCCCCCHHHHH	28.74	21082442
635	O-linked_Glycosylation	PSFDFPKTPSSQNLL CCCCCCCCCCHHHHH	28.74	9398551
637	Phosphorylation	FDFPKTPSSQNLLTL CCCCCCCCHHHHHHH	50.19	26824392
638	Phosphorylation	DFPKTPSSQNLLTLL CCCCCCCHHHHHHHH	25.04	22942356
643	Phosphorylation	PSSQNLLTLLARQGV CCHHHHHHHHHHCCC	23.92	28833060
659	Phosphorylation	MTPPRNRTLPDLSEA CCCCCCCCCCCHHHC	46.31	26824392
691	Phosphorylation	TRGPFGRSFSTSRIT CCCCCCCCCCHHHHH	24.75	26745281
693	Phosphorylation	GPFGRSFSTSRITDL CCCCCCCCHHHHHHH	27.07	26745281
694	Phosphorylation	PFGRSFSTSRITDLL CCCCCCCHHHHHHHH	21.73	23984901
695	Phosphorylation	FGRSFSTSRITDLLL CCCCCCHHHHHHHHH	22.08	26745281
708	Phosphorylation	LLKAAFGTQASDSGS HHHHHHCCCCCCCCC	17.95	25293948
711	Phosphorylation	AAFGTQASDSGSTDS HHHCCCCCCCCCCCC	23.61	26643407
713	Phosphorylation	FGTQASDSGSTDSLQ HCCCCCCCCCCCCCC	31.95	26643407
715	Phosphorylation	TQASDSGSTDSLQEK CCCCCCCCCCCCCCC	32.75	26643407
716	Phosphorylation	QASDSGSTDSLQEKP CCCCCCCCCCCCCCC	32.88	21659605
718	Phosphorylation	SDSGSTDSLQEKPME CCCCCCCCCCCCCCC	31.85	21659605
729	Phosphorylation	KPMEIAPSAGFGGTL CCCCCCCCCCCCCCC	31.81	25293948
735	Phosphorylation	PSAGFGGTLHPGARG CCCCCCCCCCCCCCC	23.44	25293948
746	Phosphorylation	GARGGGASSPAPVVF CCCCCCCCCCCCEEE	39.88	26643407
747	Phosphorylation	ARGGGASSPAPVVFT CCCCCCCCCCCEEEE	25.25	21659605
754	Phosphorylation	SPAPVVFTVGSPPSG CCCCEEEECCCCCCC	16.61	26239621
754	O-linked_Glycosylation	SPAPVVFTVGSPPSG CCCCEEEECCCCCCC	16.61	462333211
757	Phosphorylation	PVVFTVGSPPSGATP CEEEECCCCCCCCCC	29.33	26824392
760	Phosphorylation	FTVGSPPSGATPPQS EECCCCCCCCCCCCC	44.43	26745281
763	Phosphorylation	GSPPSGATPPQSTRT CCCCCCCCCCCCCCE	38.74	23684622
767	Phosphorylation	SGATPPQSTRTRMFS CCCCCCCCCCEEEEE	26.15	26643407
768	Phosphorylation	GATPPQSTRTRMFSV CCCCCCCCCEEEEEE	30.71	26643407
774	Phosphorylation	STRTRMFSVGSSSSL CCCEEEEEECCCCCC	19.07	21819378
777	Phosphorylation	TRMFSVGSSSSLGST EEEEEECCCCCCCCC	25.54	21258367
778	Phosphorylation	RMFSVGSSSSLGSTG EEEEECCCCCCCCCC	20.30	29472430
779	Phosphorylation	MFSVGSSSSLGSTGS EEEECCCCCCCCCCC	31.14	29472430
780	Phosphorylation	FSVGSSSSLGSTGSS EEECCCCCCCCCCCC	38.40	25521595
867	Phosphorylation	EIAALKGSASEAAGG HHHHHHCCHHHHCCC	27.34	19807128
913	Phosphorylation	KVAELLSSGLQTAID HHHHHHHCHHHHHHH	43.42	19807128
950	Phosphorylation	LYKASVVSCQGLSLR HHHHHHEECCHHHHH	9.89	-
1043	Phosphorylation	LCIERRLSALLSGVY HHHHHHHHHHHHCCC	18.30	29472430
1047	Phosphorylation	RRLSALLSGVYA--- HHHHHHHHCCCC---	27.36	21855797

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
180	T	Phosphorylation	Kinase	ULK1	O75385	PSP
224	S	Phosphorylation	Kinase	CDK1	P06493	PSP
317	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
317	S	Phosphorylation	Kinase	AMPK	-	Uniprot
317	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
467	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
504	S	Phosphorylation	Kinase	MAPK14	Q16539	GPS
555	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
555	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
555	S	Phosphorylation	Kinase	AMPK	-	Uniprot
622	S	Phosphorylation	Kinase	CDK1	P06493	PSP
629	S	Phosphorylation	Kinase	CDK1	P06493	PSP
635	T	Phosphorylation	Kinase	CDK1	P06493	PSP
637	S	Phosphorylation	Kinase	CDK1	P06493	PSP
637	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
637	S	Phosphorylation	Kinase	AMPK	-	Uniprot
638	S	Phosphorylation	Kinase	CDK1	P06493	PSP
653	T	Phosphorylation	Kinase	CDK1	P06493	PSP
757	S	Phosphorylation	Kinase	P38A	Q16539	PSP
757	S	Phosphorylation	Kinase	MTOR	Q9JLN9	Uniprot
757	S	Phosphorylation	Kinase	MTOR	P42345	PSP
757	S	Phosphorylation	Kinase	MAPK14	P47811	GPS
774	S	Phosphorylation	Kinase	AKT1	P31750	PSP
774	S	Phosphorylation	Kinase	AKT1	P31749	PSP
777	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
777	S	Phosphorylation	Kinase	PRKAA1	Q13131	GPS
777	S	Phosphorylation	Kinase	AMPK	-	Uniprot
983	S	Phosphorylation	Kinase	CDK1	P06493	PSP
991	S	Phosphorylation	Kinase	CDK1	P06493	PSP
1047	S	Phosphorylation	Kinase	ULK1	O75385	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ULK1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ULK1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
SQSTM_MOUSE	Sqstm1	physical	17389358
ATGA1_HUMAN	ATG101	physical	19597335

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ULK1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinaseconnects energy sensing to mitophagy."; Egan D.F., Shackelford D.B., Mihaylova M.M., Gelino S., Kohnz R.A.,Mair W., Vasquez D.S., Joshi A., Gwinn D.M., Taylor R., Asara J.M.,Fitzpatrick J., Dillin A., Viollet B., Kundu M., Hansen M., Shaw R.J.; Science 331:456-461(2011). Cited for: FUNCTION, AUTOPHOSPHORYLATION, PHOSPHORYLATION AT SER-467; SER-555;THR-574 AND SER-637, AND MUTAGENESIS OF LYS-46.	21205641 [Abstract]
"AMPK and mTOR regulate autophagy through direct phosphorylation ofUlk1."; Kim J., Kundu M., Viollet B., Guan K.L.; Nat. Cell Biol. 13:132-141(2011). Cited for: FUNCTION, AUTOPHOSPHORYLATION, PHOSPHORYLATION AT SER-317; SER-757 ANDSER-777, AND MUTAGENESIS OF LYS-46; SER-317; SER-494; SER-555;THR-574; SER-622; THR-624; SER-693; SER-757; SER-777 AND SER-811.	21258367 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-624 AND SER-637, ANDMASS SPECTROMETRY.	17242355 [Abstract]