dbPTM

UBE2O_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	UBE2O_MOUSE
UniProt AC	Q6ZPJ3
Protein Name	(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O
Gene Name	Ube2o
Organism	Mus musculus (Mouse).
Sequence Length	1288
Subcellular Localization	Cytoplasm . Nucleus . Mainly localizes to the cytoplasm.
Protein Description	E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly..
Protein Sequence	MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESTEDK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
43	Phosphorylation	DSASGPSSDSGPEAG CCCCCCCCCCCCCCC	38.64	25338131
45	Phosphorylation	ASGPSSDSGPEAGSQ CCCCCCCCCCCCCCC	59.19	25338131
51	Phosphorylation	DSGPEAGSQRLLFSH CCCCCCCCCEEEEEE	21.12	25338131
68	Phosphorylation	VSGRYRGSVHFGLVR CCCCCCCEEEEEEEE	11.92	26824392
82	Phosphorylation	RLIHGEDSDSEGDDD EEECCCCCCCCCCCC	38.44	25521595
84	Phosphorylation	IHGEDSDSEGDDDGR ECCCCCCCCCCCCCC	47.64	25521595
93	Phosphorylation	GDDDGRGSSGCSEAG CCCCCCCCCCCCCCC	23.38	22802335
94	Phosphorylation	DDDGRGSSGCSEAGG CCCCCCCCCCCCCCC	46.79	21659605
97	Phosphorylation	GRGSSGCSEAGGAGH CCCCCCCCCCCCCCC	33.72	27087446
110	Phosphorylation	GHEEGRASPLRRGYV CCCCCCCCCCCCCEE	24.09	22817900
127	Ubiquitination	QWYPEGVKQHVKETK EECCHHHHHHHHHCC	45.61	22790023
131	Ubiquitination	EGVKQHVKETKLKLE HHHHHHHHHCCCCCC	58.50	-
136	Ubiquitination	HVKETKLKLEDRSVV HHHHCCCCCCCCCCC	51.92	27667366
171	Ubiquitination	VNIDCAVKLIGTNCI EECCCEEEEECCCEE	18.83	-
212	Ubiquitination	LGKVYDLKNQIILKL CCEEEECCCCEEEEE	43.86	22790023
255	Ubiquitination	FFDDSYGFYPGQVLI EECCCCCCCCCCEEE	5.16	27667366
269	Phosphorylation	IGPAKIFSSVQWLSG EECHHHHHCCHHHHC	32.83	-
298	Ubiquitination	VEEVQVVELKVTWIT EEEEEEEEEEEEEEE	43.97	27667366
383	Ubiquitination	SMAKKVKRLLKKQVV HHHHHHHHHHHHHCE	48.52	27667366
393	Ubiquitination	KKQVVRIMSCTPDTQ HHHCEEEEECCCCCC	1.56	27667366
394	Phosphorylation	KQVVRIMSCTPDTQC HHCEEEEECCCCCCC	16.68	25521595
396	Phosphorylation	VVRIMSCTPDTQCPR CEEEEECCCCCCCCC	19.52	25367039
399	Phosphorylation	IMSCTPDTQCPRDHS EEECCCCCCCCCCCC	33.02	25367039
406	Phosphorylation	TQCPRDHSMEDPDKK CCCCCCCCCCCCCCC	28.31	25521595
420	Phosphorylation	KGEARAGSEIGSASP CCCCCCCCCCCCCCC	25.67	25293948
424	Phosphorylation	RAGSEIGSASPEEQP CCCCCCCCCCCCCCC	31.10	19060867
426	Phosphorylation	GSEIGSASPEEQPDG CCCCCCCCCCCCCCC	34.24	22817900
430	Ubiquitination	GSASPEEQPDGSASP CCCCCCCCCCCCCCC	39.46	27667366
434	Phosphorylation	PEEQPDGSASPVEMQ CCCCCCCCCCCCCCC	32.71	25293948
436	Phosphorylation	EQPDGSASPVEMQDE CCCCCCCCCCCCCCC	31.04	22817900
445	Phosphorylation	VEMQDEGSEELQETC CCCCCCCCHHHHHHC	26.33	21183079
451	Phosphorylation	GSEELQETCEPLPPF CCHHHHHHCCCCCCE	14.81	25293948
470	Phosphorylation	GGDDGLHSAEQDADD CCCCCCCCCCCCCCC	38.25	26525534
483	Phosphorylation	DDEAADDTDDTSSVT CCCCCCCCCCCCCEE	36.00	22817900
486	Phosphorylation	AADDTDDTSSVTSSA CCCCCCCCCCEECCC	26.22	-
487	Phosphorylation	ADDTDDTSSVTSSAS CCCCCCCCCEECCCC	29.79	22817900
488	Phosphorylation	DDTDDTSSVTSSASS CCCCCCCCEECCCCC	31.35	-
491	Ubiquitination	DDTSSVTSSASSTTS CCCCCEECCCCCCCC	23.04	27667366
492	Phosphorylation	DTSSVTSSASSTTSS CCCCEECCCCCCCCC	23.86	27087446
494	Phosphorylation	SSVTSSASSTTSSQS CCEECCCCCCCCCCC	30.11	-
501	Phosphorylation	SSTTSSQSGSGTGRK CCCCCCCCCCCCCCC	36.55	-
510	Phosphorylation	SGTGRKKSIPLSIKN CCCCCCCCCCCHHHH	32.26	25521595
514	Phosphorylation	RKKSIPLSIKNLKRK CCCCCCCHHHHHHHH	26.60	25619855
516	Ubiquitination	KSIPLSIKNLKRKHK CCCCCHHHHHHHHHH	53.50	22790023
588	Ubiquitination	PGDFVVDKRVQSCPD CCCEEEECCCCCCCC	42.74	22790023
638	Phosphorylation	EEEDVSVYDIADHPD CCCCCEEEECCCCCC	8.30	22817900
667	Ubiquitination	TEDGALPKEDEPSVG CCCCCCCCCCCCCCC	77.85	22790023
706	Ubiquitination	PQHLYNIESEIEESD CHHHCCCCCCCCCCC	38.39	27667366
787	Ubiquitination	AAIQGAVAMAAPVAG HHHHHHHHHHHHHHH	4.95	27667366
812	Ubiquitination	PKSFRELKEAIKILE CHHHHHHHHHHHHHH	40.11	22790023
816	Ubiquitination	RELKEAIKILESLKN HHHHHHHHHHHHHCC	48.57	22790023
822	Ubiquitination	IKILESLKNMTVEQL HHHHHHHCCCCHHHH	54.69	22790023
824	Oxidation	ILESLKNMTVEQLLT HHHHHCCCCHHHHHH	4.20	17242355
825	Phosphorylation	LESLKNMTVEQLLTG HHHHCCCCHHHHHHC	30.22	24925903
830	Ubiquitination	NMTVEQLLTGSPTSP CCCHHHHHHCCCCCC	5.21	27667366
831	Phosphorylation	MTVEQLLTGSPTSPT CCHHHHHHCCCCCCC	44.18	24925903
833	Phosphorylation	VEQLLTGSPTSPTVE HHHHHHCCCCCCCCC	21.55	25521595
835	Phosphorylation	QLLTGSPTSPTVEPE HHHHCCCCCCCCCCC	49.38	25521595
836	Phosphorylation	LLTGSPTSPTVEPEK HHHCCCCCCCCCCCC	23.20	25521595
838	Phosphorylation	TGSPTSPTVEPEKPT HCCCCCCCCCCCCCC	36.95	25521595
843	Ubiquitination	SPTVEPEKPTREKKF CCCCCCCCCCCHHHH	63.50	22790023
845	Phosphorylation	TVEPEKPTREKKFLD CCCCCCCCCHHHHHH	63.63	25619855
849	Ubiquitination	EKPTREKKFLDDIKK CCCCCHHHHHHHHHH	46.54	22790023
855	Ubiquitination	KKFLDDIKKLQENLK HHHHHHHHHHHHHHH	55.19	27667366
863	Ubiquitination	KLQENLKKTLDNVAI HHHHHHHHHHHHHHH	58.44	22790023
864	Phosphorylation	LQENLKKTLDNVAIA HHHHHHHHHHHHHHH	37.44	29899451
882	Phosphorylation	KMEAVPDTERKEEKP HHHCCCCCCCCCCCC	31.45	29899451
893	Phosphorylation	EEKPEVQSPVKAEWP CCCCCCCCCCCCCCC	36.51	25521595
901	Phosphorylation	PVKAEWPSETPVLCQ CCCCCCCCCCCEEHH	55.99	25777480
903	Phosphorylation	KAEWPSETPVLCQQC CCCCCCCCCEEHHHC	24.04	25777480
915	Ubiquitination	QQCGGRPGVTFTSAK HHCCCCCCEEEECCC	29.72	27667366
917	Phosphorylation	CGGRPGVTFTSAKGE CCCCCCEEEECCCCC	27.41	25777480
919	Phosphorylation	GRPGVTFTSAKGEVF CCCCEEEECCCCCEE	20.57	25777480
920	Phosphorylation	RPGVTFTSAKGEVFS CCCEEEECCCCCEEE	24.64	25777480
925	Ubiquitination	FTSAKGEVFSVLEFA EECCCCCEEEEEEEC	6.21	27667366
940	Ubiquitination	PSNHSFKKIEFQPPE CCCCCCCEEEECCHH	45.52	22790023
950	Ubiquitination	FQPPEAKKFFSTVRK ECCHHHHHHHHHHHH	59.70	27667366
962	Ubiquitination	VRKEMALLATSLPDG HHHHHHHHHHCCCCC	3.38	27667366
987	Ubiquitination	DLFSALIKGPTRTPY HHHHHHHCCCCCCCC	60.74	22790023
1108	Phosphorylation	ALIRVVQSMTQLVRR HHHHHHHHHHHHHHC	16.52	20531401
1110	Phosphorylation	IRVVQSMTQLVRRPP HHHHHHHHHHHHCCH	24.81	20531401
1163	Phosphorylation	PNGALKDSSSLEPMA CCCCCCCCCCCCHHH	21.90	24759943
1164	Phosphorylation	NGALKDSSSLEPMAA CCCCCCCCCCCHHHH	49.42	24759943
1165	Phosphorylation	GALKDSSSLEPMAAA CCCCCCCCCCHHHHH	40.48	25521595
1175	Phosphorylation	PMAAAELSDSGREEP HHHHHHCCCCCCCCC	22.64	25619855
1177	Phosphorylation	AAAELSDSGREEPED HHHHCCCCCCCCCCC	36.05	25619855
1193	Phosphorylation	GMAPGEASQGSDSEG CCCCCCCCCCCCCCC	30.67	21082442
1196	Phosphorylation	PGEASQGSDSEGGAQ CCCCCCCCCCCCCCC	30.26	25521595
1198	Phosphorylation	EASQGSDSEGGAQGP CCCCCCCCCCCCCCC	39.58	25619855
1207	Phosphorylation	GGAQGPASASRDHTE CCCCCCCCCCCCCCC	29.88	25619855
1209	Phosphorylation	AQGPASASRDHTEQT CCCCCCCCCCCCCCC	35.43	25619855
1223	Phosphorylation	TETAPDASAPPSVRP CCCCCCCCCCCCCCC	49.00	28066266
1227	Phosphorylation	PDASAPPSVRPKRRR CCCCCCCCCCCCCCH	29.69	23684622
1236	Phosphorylation	RPKRRRKSYRSFLPE CCCCCHHHHHHHCCC	24.78	22324799
1237	Phosphorylation	PKRRRKSYRSFLPEK CCCCHHHHHHHCCCC	17.20	29472430
1238	Ubiquitination	KRRRKSYRSFLPEKS CCCHHHHHHHCCCCC	28.20	27667366
1239	Phosphorylation	RRRKSYRSFLPEKSG CCHHHHHHHCCCCCC	23.93	26643407
1259	Ubiquitination	FPLFPLSKGFIKSIR CCCCCCCHHHHHHHH	66.57	-
1263	Ubiquitination	PLSKGFIKSIRGVLT CCCHHHHHHHHHHHH	37.25	27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of UBE2O_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of UBE2O_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of UBE2O_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
CPNE1_HUMAN	CPNE1	physical	12522145
CPNE4_HUMAN	CPNE4	physical	12522145

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of UBE2O_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82; SER-84 AND SER-893,AND MASS SPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82; SER-84; SER-833 ANDSER-836, AND MASS SPECTROMETRY.	17242355 [Abstract]