UBC12_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBC12_MOUSE
• UniProt AC: P61082
• Protein Name: NEDD8-conjugating enzyme Ubc12
• Gene Name: Ube2m
• Organism: Mus musculus (Mouse).
• Sequence Length: 183
• Protein Description: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (By similarity)..
• Protein Sequence: MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MIKLFSLK -------CCCCCCCC	5.71	-
3	Acetylation	-----MIKLFSLKQQ -----CCCCCCCCHH	42.03	22826441
3	Ubiquitination	-----MIKLFSLKQQ -----CCCCCCCCHH	42.03	22790023
6	Phosphorylation	--MIKLFSLKQQKKE --CCCCCCCCHHHHH	45.13	22942356
8	Ubiquitination	MIKLFSLKQQKKEEE CCCCCCCCHHHHHHH	49.86	22790023
8	Acetylation	MIKLFSLKQQKKEEE CCCCCCCCHHHHHHH	49.86	22826441
28	Phosphorylation	KGSSKKASAAQLRIQ CCCCHHHHHHHHHHH	32.77	30352176
36	Ubiquitination	AAQLRIQKDINELNL HHHHHHHHCHHHCCC	59.20	22790023
36	Acetylation	AAQLRIQKDINELNL HHHHHHHHCHHHCCC	59.20	22826441
45	Ubiquitination	INELNLPKTCDISFS HHHCCCCCCCCCCCC	66.02	-
50	Phosphorylation	LPKTCDISFSDPDDL CCCCCCCCCCCHHHH	12.87	26745281
52	Phosphorylation	KTCDISFSDPDDLLN CCCCCCCCCHHHHCC	41.25	26745281
72	Ubiquitination	CPDEGFYKSGKFVFS CCCCCCCCCCEEEEE	50.93	22790023
75	Ubiquitination	EGFYKSGKFVFSFKV CCCCCCCEEEEEEEE	45.50	22790023
92	Ubiquitination	GYPHDPPKVKCETMV CCCCCCCCCEEEEEE	60.77	-
97	Phosphorylation	PPKVKCETMVYHPNI CCCCEEEEEEECCCC	22.77	30635358
100	Phosphorylation	VKCETMVYHPNIDLE CEEEEEEECCCCCCC	10.75	30635358
169	Methylation	QNVQRSMRGGYIGST HHHHHHHCCCCCCHH	36.31	24129315
169	Asymmetric dimethylarginine	QNVQRSMRGGYIGST HHHHHHHCCCCCCHH	36.31	-
172	Phosphorylation	QRSMRGGYIGSTYFE HHHHCCCCCCHHHHH	12.63	25367039
175	Phosphorylation	MRGGYIGSTYFERCL HCCCCCCHHHHHHHC	15.46	25367039
177	Phosphorylation	GGYIGSTYFERCLK- CCCCCHHHHHHHCC-	12.73	29514104
181	Glutathionylation	GSTYFERCLK----- CHHHHHHHCC-----	4.47	24333276

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of UBC12_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
1	M	Acetylation	The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBC12_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CPNE1_HUMAN	CPNE1	physical	12522145
CPNE4_HUMAN	CPNE4	physical	12522145

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.