TY3H_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TY3H_RAT
• UniProt AC: P04177
• Protein Name: Tyrosine 3-monooxygenase
• Gene Name: Th
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 498
• Protein Description: Plays an important role in the physiology of adrenergic neurons..
• Protein Sequence: MPTPSAPSPQPKGFRRAVSEQDAKQAEAVTSPRFIGRRQSLIEDARKEREAAAAAAAAAVASSEPGNPLEAVVFEERDGNAVLNLLFSLRGTKPSSLSRAVKVFETFEAKIHHLETRPAQRPLAGSPHLEYFVRFEVPSGDLAALLSSVRRVSDDVRSAREDKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYKHGEPIPHVEYTAEEIATWKEVYVTLKGLYATHACREHLEGFQLLERYCGYREDSIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTVYWFTVEFGLCKQNGELKAYGAGLLSSYGELLHSLSEEPEVRAFDPDTAAVQPYQDQTYQPVYFVSESFNDAKDKLRNYASRIQRPFSVKFDPYTLAIDVLDSPHTIQRSLEGVQDELHTLAHALSAIS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MPTPSAPSPQ -----CCCCCCCCCC	28.69	23984901
5	Phosphorylation	---MPTPSAPSPQPK ---CCCCCCCCCCCC	56.12	23984901
8	Phosphorylation	MPTPSAPSPQPKGFR CCCCCCCCCCCCCHH	35.56	23589303
19	Phosphorylation	KGFRRAVSEQDAKQA CCHHHCCCHHHHHHH	28.67	1672315
30	Phosphorylation	AKQAEAVTSPRFIGR HHHHHHCCCHHHHCH	39.81	25403869
31	Phosphorylation	KQAEAVTSPRFIGRR HHHHHCCCHHHHCHH	13.89	1672315
40	Phosphorylation	RFIGRRQSLIEDARK HHHCHHHHHHHHHHH	29.51	1672315
153	Phosphorylation	LSSVRRVSDDVRSAR HHHHHHHCHHHHHHH	26.70	22817900
177	Glutathionylation	KVSELDKCHHLVTKF CHHHHHHCCCHHHCC	2.14	22833525
249	Glutathionylation	GLYATHACREHLEGF HHHHHHHHHHHHHHH	3.99	22833525
263	Glutathionylation	FQLLERYCGYREDSI HHHHHHHCCCCCCCC	4.90	22833525
329	Glutathionylation	MHSPEPDCCHELLGH CCCCCCCHHHHHHCC	3.59	22833525
330	Glutathionylation	HSPEPDCCHELLGHV CCCCCCHHHHHHCCH	3.49	22833525
380	Glutathionylation	FTVEFGLCKQNGELK EEEEECEEECCCEEE	4.33	22833525
423	Nitration	DTAAVQPYQDQTYQP CCCCCCCCCCCCCCC	13.97	-
428	Nitration	QPYQDQTYQPVYFVS CCCCCCCCCCEEEEE	12.75	-
432	Nitration	DQTYQPVYFVSESFN CCCCCCEEEEECCCH	12.63	-
457	Phosphorylation	SRIQRPFSVKFDPYT HHCCCCCCCCCCCCE	27.38	23984901
463	Phosphorylation	FSVKFDPYTLAIDVL CCCCCCCCEEEEEEC	19.07	23984901
464	Phosphorylation	SVKFDPYTLAIDVLD CCCCCCCEEEEEECC	18.33	23984901
472	Phosphorylation	LAIDVLDSPHTIQRS EEEEECCCHHHHHHH	17.89	23589303

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
8	S	Phosphorylation	Kinase	CDK_GROUP	-	PhosphoELM
8	S	Phosphorylation	Kinase	CDK-FAMILY	-	GPS
8	S	Phosphorylation	Kinase	ERK2	P28482	PSP
8	S	Phosphorylation	Kinase	MAPK1	P63086	GPS
19	S	Phosphorylation	Kinase	CAM-KII_GROUP	-	PhosphoELM
19	S	Phosphorylation	Kinase	CAMK2	-	Uniprot
19	S	Phosphorylation	Kinase	CAMK1	Q14012	GPS
19	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
19	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
19	S	Phosphorylation	Kinase	CAMK2B	P08413	GPS
31	S	Phosphorylation	Kinase	MAPK3	P21708	GPS
31	S	Phosphorylation	Kinase	MAP2K1	Q01986	GPS
31	S	Phosphorylation	Kinase	CDK5	Q03114	PSP
31	S	Phosphorylation	Kinase	ERK1	Q63844	PSP
31	S	Phosphorylation	Kinase	ERK2	P63085	PSP
31	S	Phosphorylation	Kinase	ERK2	P28482	PSP
31	S	Phosphorylation	Kinase	PRKCB	P68403	GPS
31	S	Phosphorylation	Kinase	MAPK1	P63086	GPS
40	S	Phosphorylation	Kinase	PKACA	P17612	PSP
40	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
40	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
40	S	Phosphorylation	Kinase	CAM-KII_GROUP	-	PhosphoELM
40	S	Phosphorylation	Kinase	PKA	-	Uniprot
40	S	Phosphorylation	Kinase	CAMK2	-	Uniprot
40	S	Phosphorylation	Kinase	PRKACA	P27791	GPS
40	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
40	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
40	S	Phosphorylation	Kinase	CAMK1	Q14012	GPS
40	S	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
40	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
40	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
153	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
153	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
153	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
19	S	Phosphorylation	In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.	1672315
40	S	Phosphorylation	In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.	1672315

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TY3H_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SYUA_RAT	Snca	physical	11943812

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Phosphorylation of Ser(19) alters the conformation of tyrosinehydroxylase to increase the rate of phosphorylation of Ser(40).";
Bevilaqua L.R., Graham M.E., Dunkley P.R., von Nagy-Felsobuki E.I.,Dickson P.W.;
J. Biol. Chem. 276:40411-40416(2001).
Cited for: PHOSPHORYLATION AT SER-19 AND SER-40, AND MASS SPECTROMETRY.
PubMed: 11502746

"Tyrosine hydroxylase in rat brain dopaminergic nerve terminals.Multiple-site phosphorylation in vivo and in synaptosomes.";
Haycock J.W., Haycock D.A.;
J. Biol. Chem. 266:5650-5657(1991).
Cited for: PHOSPHORYLATION AT SER-19; SER-31 AND SER-40.
PubMed: 1672315