TRPA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRPA1_HUMAN
• UniProt AC: O75762
• Protein Name: Transient receptor potential cation channel subfamily A member 1
• Gene Name: TRPA1
• Organism: Homo sapiens (Human).
• Sequence Length: 1119
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein .
• Protein Description: Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. [PubMed: 25389312]
• Protein Sequence: MKRSLRKMWRPGEKKEPQGVVYEDVPDDTEDFKESLKVVFEGSAYGLQNFNKQKKLKRCDDMDTFFLHYAAAEGQIELMEKITRDSSLEVLHEMDDYGNTPLHCAVEKNQIESVKFLLSRGANPNLRNFNMMAPLHIAVQGMNNEVMKVLLEHRTIDVNLEGENGNTAVIIACTTNNSEALQILLKKGAKPCKSNKWGCFPIHQAAFSGSKECMEIILRFGEEHGYSRQLHINFMNNGKATPLHLAVQNGDLEMIKMCLDNGAQIDPVEKGRCTAIHFAATQGATEIVKLMISSYSGSVDIVNTTDGCHETMLHRASLFDHHELADYLISVGADINKIDSEGRSPLILATASASWNIVNLLLSKGAQVDIKDNFGRNFLHLTVQQPYGLKNLRPEFMQMQQIKELVMDEDNDGCTPLHYACRQGGPGSVNNLLGFNVSIHSKSKDKKSPLHFAASYGRINTCQRLLQDISDTRLLNEGDLHGMTPLHLAAKNGHDKVVQLLLKKGALFLSDHNGWTALHHASMGGYTQTMKVILDTNLKCTDRLDEDGNTALHFAAREGHAKAVALLLSHNADIVLNKQQASFLHLALHNKRKEVVLTIIRSKRWDECLKIFSHNSPGNKCPITEMIEYLPECMKVLLDFCMLHSTEDKSCRDYYIEYNFKYLQCPLEFTKKTPTQDVIYEPLTALNAMVQNNRIELLNHPVCKEYLLMKWLAYGFRAHMMNLGSYCLGLIPMTILVVNIKPGMAFNSTGIINETSDHSEILDTTNSYLIKTCMILVFLSSIFGYCKEAGQIFQQKRNYFMDISNVLEWIIYTTGIIFVLPLFVEIPAHLQWQCGAIAVYFYWMNFLLYLQRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDPFSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTSLEKKLPLWFLRKVDQKSTIVYPNKPRSGGMLFHIFCFLFCTGEIRQEIPNADKSLEMEILKQKYRLKDLTFLLEKQHELIKLIIQKMEIISETEDDDSHCSFQDRFKKEQMEQRNSRWNTVLRAVKAKTHHLEP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
35	Phosphorylation	DTEDFKESLKVVFEG CHHHHHHHHCHHCCC	33.80	26657352
43	Phosphorylation	LKVVFEGSAYGLQNF HCHHCCCCHHHHHCC	15.89	28348404
86	Phosphorylation	MEKITRDSSLEVLHE HHHHHCCCCHHHHHC	33.27	28348404
87	Phosphorylation	EKITRDSSLEVLHEM HHHHCCCCHHHHHCC	32.60	28348404
113	Phosphorylation	VEKNQIESVKFLLSR EECCHHHHHHHHHHC	32.03	25278378
304	Phosphorylation	GSVDIVNTTDGCHET CCEEEEECCCCCCHH	18.50	-
305	Phosphorylation	SVDIVNTTDGCHETM CEEEEECCCCCCHHH	25.78	-
317	Phosphorylation	ETMLHRASLFDHHEL HHHCHHHHCCCHHHH	29.53	-
337	Methylation	SVGADINKIDSEGRS HCCCCHHHCCCCCCC	48.50	-
344	Phosphorylation	KIDSEGRSPLILATA HCCCCCCCCEEEEEC	34.61	30301811
350	Phosphorylation	RSPLILATASASWNI CCCEEEEECCCCHHH	20.07	30301811
352	Phosphorylation	PLILATASASWNIVN CEEEEECCCCHHHHH	20.62	30301811
354	Phosphorylation	ILATASASWNIVNLL EEEECCCCHHHHHHH	20.40	30301811
363	Phosphorylation	NIVNLLLSKGAQVDI HHHHHHHHCCCCEEC	29.07	-
394	Hydroxylation	YGLKNLRPEFMQMQQ CCHHHCCHHHHHHHH	42.20	21873995
403	Acetylation	FMQMQQIKELVMDED HHHHHHHHHHHCCCC	40.43	19826671
428	Phosphorylation	CRQGGPGSVNNLLGF HHCCCCCCHHHHHCC	25.46	-
438	Phosphorylation	NLLGFNVSIHSKSKD HHHCCEEEEECCCCC	18.71	27251275
448	Phosphorylation	SKSKDKKSPLHFAAS CCCCCCCCCHHHHHH	38.13	28348404
455	Phosphorylation	SPLHFAASYGRINTC CCHHHHHHHCCHHHH	25.37	24719451
470	Phosphorylation	QRLLQDISDTRLLNE HHHHHHCCCCCCCCC	41.02	20363803
503	Methylation	KVVQLLLKKGALFLS HHHHHHHHCCCEEEE	49.48	23644510
504	Methylation	VVQLLLKKGALFLSD HHHHHHHCCCEEEEC	49.76	23644510
633	Oxidation	MIEYLPECMKVLLDF HHHHHHHHHHHHHHH	2.78	21873995
658	Phosphorylation	CRDYYIEYNFKYLQC HHHEEEEEEEEEEEC	19.15	29759185
673	Phosphorylation	PLEFTKKTPTQDVIY CCCCCCCCCCCCCCC	32.88	-
747	N-linked_Glycosylation	IKPGMAFNSTGIINE ECCCCCCCCCCCCCC	29.17	UniProtKB CARBOHYD
753	N-linked_Glycosylation	FNSTGIINETSDHSE CCCCCCCCCCCCCHH	44.57	UniProtKB CARBOHYD
764	Phosphorylation	DHSEILDTTNSYLIK CCHHHHHHCHHHHHH	25.16	-
856	Oxidation	YLQRFENCGIFIVML HHHHHHCCCHHHHHH	3.30	21873995
972	Phosphorylation	AEVQKHASLKRIAMQ HHHHHHHHHHHHHHH	33.86	-
1076	Phosphorylation	IQKMEIISETEDDDS HHHHCHHCCCCCCCC	44.09	28348404
1078	Phosphorylation	KMEIISETEDDDSHC HHCHHCCCCCCCCCC	37.61	28348404
1083	Phosphorylation	SETEDDDSHCSFQDR CCCCCCCCCCCHHHH	33.13	23879269
1086	Phosphorylation	EDDDSHCSFQDRFKK CCCCCCCCHHHHHHH	22.10	23879269

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
86	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
317	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
428	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
972	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
633	C	Hydroxylation	Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.	21873995
633	C	Oxidation	Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.	21873995
856	C	Hydroxylation	Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.	21873995
856	C	Oxidation	Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.	21873995

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRPA1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AKAP5_HUMAN	AKAP5	physical	18701070

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 615040: Episodic pain syndrome, familial, 1 (FEPS1)
• Kegg Drug
• D00064: l-Menthol (JP16); Levomenthol; l-Menthol (TN)
• D00098: dl-Camphor (JP16); Camphor (USP)
• D04849: dl-Menthol (JP16)
• D04918: Menthol (USP); Fisherman's friend lozenges (TN)
• DrugBank
• There are no disease associations of PTM sites.