TRIPC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRIPC_MOUSE
• UniProt AC: G5E870
• Protein Name: E3 ubiquitin-protein ligase TRIP12
• Gene Name: Trip12
• Organism: Mus musculus (Mouse).
• Sequence Length: 2025
• Subcellular Localization: Nucleus, nucleoplasm.
• Protein Description: E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes..
• Protein Sequence: MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPRAFQHIESFSETNKPHSKSKKRHLDQEQQLKSAQLPSTSKAHTRKSVAAGSSRNQKRKRTESSCVKSGSGSESTGAEERSAKPIKLASKSATSAKAGCSTITDSSSAASTSSSSSAIASASSTVPAGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQETVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTPASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSNRPNNNP ------CCCCCCCCC		-
12	Phosphorylation	PNNNPGGSLRRSQRN CCCCCCCCCCHHHCC		28418008
20	Phosphorylation	LRRSQRNTAGAQPQD CCHHHCCCCCCCCCC		-
34	Phosphorylation	DDSIGGRSCSSSSAV CCCCCCCCCCCCCCE		30635358
36	Phosphorylation	SIGGRSCSSSSAVIV CCCCCCCCCCCCEEE		30635358
37	Phosphorylation	IGGRSCSSSSAVIVP CCCCCCCCCCCEEEC		30635358
38	Phosphorylation	GGRSCSSSSAVIVPQ CCCCCCCCCCEEECC		30635358
39	Phosphorylation	GRSCSSSSAVIVPQP CCCCCCCCCEEECCC		30635358
54	Phosphorylation	EDPDRANTSERQKTG CCCCCCCCCHHHHCC		22802335
75	Phosphorylation	NSRGVKRSASPDYNR CCCCCCCCCCCCCCC		27087446
77	Phosphorylation	RGVKRSASPDYNRTN CCCCCCCCCCCCCCC		27087446
80	Phosphorylation	KRSASPDYNRTNSPS CCCCCCCCCCCCCCC		22324799
83	Phosphorylation	ASPDYNRTNSPSSAK CCCCCCCCCCCCCCC		22324799
85	Phosphorylation	PDYNRTNSPSSAKKP CCCCCCCCCCCCCCC		23684622
87	Phosphorylation	YNRTNSPSSAKKPRA CCCCCCCCCCCCCCH		22324799
88	Phosphorylation	NRTNSPSSAKKPRAF CCCCCCCCCCCCCHH		29472430
100	Phosphorylation	RAFQHIESFSETNKP CHHHHHHHHHHCCCC		29899451
154	Phosphorylation	QKRKRTESSCVKSGS HHCCCCHHHCCCCCC		23684622
159	Phosphorylation	TESSCVKSGSGSEST CHHHCCCCCCCCCCC		25266776
161	Phosphorylation	SSCVKSGSGSESTGA HHCCCCCCCCCCCCC		23684622
163	Phosphorylation	CVKSGSGSESTGAEE CCCCCCCCCCCCCCH		28066266
165	Phosphorylation	KSGSGSESTGAEERS CCCCCCCCCCCCHHC		28066266
181	Acetylation	KPIKLASKSATSAKA CCCEECCCCCCCCCC		23806337
196	Phosphorylation	GCSTITDSSSAASTS CCCCCCCCCCCCCCC		-
197	Phosphorylation	CSTITDSSSAASTSS CCCCCCCCCCCCCCC		-
211	Phosphorylation	SSSSAIASASSTVPA CCCCCHHHCCCCCCC		-
234	Phosphorylation	DQNKARRSRSASSPS CCHHHHHHHCCCCCC		30635358
236	Phosphorylation	NKARRSRSASSPSPR HHHHHHHCCCCCCCC		30635358
238	Phosphorylation	ARRSRSASSPSPRRS HHHHHCCCCCCCCCC		29550500
239	Phosphorylation	RRSRSASSPSPRRSS HHHHCCCCCCCCCCH		27818261
241	Phosphorylation	SRSASSPSPRRSSRE HHCCCCCCCCCCHHH		29899451
245	Phosphorylation	SSPSPRRSSREKEQS CCCCCCCCHHHHHHH		-
246	Phosphorylation	SPSPRRSSREKEQSK CCCCCCCHHHHHHHC		-
267	Phosphorylation	FDWAARFSPKVSLPK CCHHHHCCCCCCCCC		26643407
276	Ubiquitination	KVSLPKTKLSLPGSS CCCCCCCCCCCCCCC		27667366
278	Phosphorylation	SLPKTKLSLPGSSKS CCCCCCCCCCCCCCC		29176673
301	Phosphorylation	GLQAKLASLRKSTKK HHHHHHHHHHHHCCC		29514104
305	Phosphorylation	KLASLRKSTKKRSES HHHHHHHHCCCCCCC		29514104
310	Phosphorylation	RKSTKKRSESPPAEL HHHCCCCCCCCCCCC		27087446
312	Phosphorylation	STKKRSESPPAELPS HCCCCCCCCCCCCCH		27087446
318	Ubiquitination	ESPPAELPSLRRSTR CCCCCCCCHHHHCCC		27667366
319	Phosphorylation	SPPAELPSLRRSTRQ CCCCCCCHHHHCCCC		25619855
340	Phosphorylation	ASTSRRGSGLGKRGA CCCCCCCCCCCHHHH		23737553
398	Phosphorylation	GMTTSGESESDDSEM CCCCCCCCCCCCCHH		24899341
430	Phosphorylation	GPLGPRMSQLFHRTI CCCCHHHHHHHHHHH		29472430
754	Phosphorylation	EGIFAVDTMLKKGNA CCEEEHHHHHHCCCC		-
781	Phosphorylation	DRGLWHPYNRIDSRI CCCCCCCCCCHHHHH		29514104
839	Phosphorylation	KPNPLANSNTSGYSE CCCCCCCCCCCCCHH		30635358
841	Phosphorylation	NPLANSNTSGYSELK CCCCCCCCCCCHHHC		30635358
842	Phosphorylation	PLANSNTSGYSELKK CCCCCCCCCCHHHCH		30635358
844	Phosphorylation	ANSNTSGYSELKKDD CCCCCCCCHHHCHHH		30635358
845	Phosphorylation	NSNTSGYSELKKDDA CCCCCCCHHHCHHHH		30635358
876	Phosphorylation	KTLFGVLYEVYSSSA HHHHHHHHHHHHCCC		21183079
882	Phosphorylation	LYEVYSSSAGPAVRH HHHHHHCCCCHHHHH		22817900
918	Phosphorylation	LKNHAVSSHIASMLS HHHHHHHHHHHHHHC		-
969	Phosphorylation	QVKHLAESESLLTSP HHHHHHHCHHHCCCC		25159016
974	Phosphorylation	AESESLLTSPPKACT HHCHHHCCCCCCCCC		24068923
975	Phosphorylation	ESESLLTSPPKACTN HCHHHCCCCCCCCCC		26824392
981	Phosphorylation	TSPPKACTNGSGSLG CCCCCCCCCCCCCCC		25293948
984	Phosphorylation	PKACTNGSGSLGSTT CCCCCCCCCCCCCCC		25293948
986	Phosphorylation	ACTNGSGSLGSTTPA CCCCCCCCCCCCCCC		25293948
989	Phosphorylation	NGSGSLGSTTPASSG CCCCCCCCCCCCCCC		25293948
990	Phosphorylation	GSGSLGSTTPASSGT CCCCCCCCCCCCCCC		25293948
991	Phosphorylation	SGSLGSTTPASSGTA CCCCCCCCCCCCCCC		25293948
994	Phosphorylation	LGSTTPASSGTATAA CCCCCCCCCCCCCCC		25293948
995	Phosphorylation	GSTTPASSGTATAAT CCCCCCCCCCCCCCC		25293948
997	Phosphorylation	TTPASSGTATAATNA CCCCCCCCCCCCCCC		25293948
999	Phosphorylation	PASSGTATAATNASA CCCCCCCCCCCCCCC		25293948
1002	Phosphorylation	SGTATAATNASADLG CCCCCCCCCCCCCCC		25293948
1005	Phosphorylation	ATAATNASADLGSPS CCCCCCCCCCCCCCC		25293948
1010	Phosphorylation	NASADLGSPSLQHSR CCCCCCCCCCCCCCC		25293948
1012	Phosphorylation	SADLGSPSLQHSRDD CCCCCCCCCCCCCCC		25293948
1016	Phosphorylation	GSPSLQHSRDDSLDL CCCCCCCCCCCCCCC		25293948
1020	Phosphorylation	LQHSRDDSLDLSPQG CCCCCCCCCCCCCCC		25619855
1024	Phosphorylation	RDDSLDLSPQGRLSD CCCCCCCCCCCCHHH		26824392
1030	Phosphorylation	LSPQGRLSDVLKRKR CCCCCCHHHHHHHCC		26824392
1048	Phosphorylation	RGPRRPKYSPPRDDD CCCCCCCCCCCCCCC		27742792
1049	Phosphorylation	GPRRPKYSPPRDDDK CCCCCCCCCCCCCCC		26824392
1063	Phosphorylation	KVDNQAKSPTTTQSP CCCCCCCCCCCCCCC		27087446
1065	Phosphorylation	DNQAKSPTTTQSPKS CCCCCCCCCCCCCCH		22324799
1066	Phosphorylation	NQAKSPTTTQSPKSS CCCCCCCCCCCCCHH		23684622
1067	Phosphorylation	QAKSPTTTQSPKSSF CCCCCCCCCCCCHHH		23684622
1069	Phosphorylation	KSPTTTQSPKSSFLA CCCCCCCCCCHHHHH		27087446
1071	Ubiquitination	PTTTQSPKSSFLASL CCCCCCCCHHHHHHC		22790023
1077	Phosphorylation	PKSSFLASLNPKTWG CCHHHHHHCCCCCHH		29514104
1081	Ubiquitination	FLASLNPKTWGRLSA HHHHCCCCCHHHCCC		22790023
1087	Phosphorylation	PKTWGRLSAQSNSNN CCCHHHCCCCCCCCC		28066266
1090	Phosphorylation	WGRLSAQSNSNNIEP HHHCCCCCCCCCCCH		28066266
1092	Phosphorylation	RLSAQSNSNNIEPAR HCCCCCCCCCCCHHH		26026062
1111	Phosphorylation	SGLARAASKDTISNN HHHHHHHCCCCHHCC		25159016
1114	Phosphorylation	ARAASKDTISNNREK HHHHCCCCHHCCHHH		25159016
1146	Phosphorylation	SSENMDGSNPALNVL CCCCCCCCCHHHHHH		29514104
1203	Phosphorylation	FVKQLLLYLTSKNEK HHHHHHHHHHCCCCC		-
1260	Ubiquitination	HKMNNCLSQMEQFPV HHHHHHHHHHHHCCC		27667366
1274	Phosphorylation	VKVHDFPSGNGAGGS CEEECCCCCCCCCCC		25293948
1281	Phosphorylation	SGNGAGGSFSLNRGS CCCCCCCCCCCCCCC		25266776
1283	Phosphorylation	NGAGGSFSLNRGSQA CCCCCCCCCCCCCHH		26370283
1292	Ubiquitination	NRGSQALKFFNTHQL CCCCHHHHHCCHHHH		22790023
1321	Ubiquitination	QWKGGPVKIDPLALV CCCCCCCCCCHHHHH		22790023
1350	Phosphorylation	VREDDEDSDDDGSDE CCCCCCCCCCCCCHH		27087446
1355	Phosphorylation	EDSDDDGSDEEIDES CCCCCCCCHHHHHHH		27087446
1362	Phosphorylation	SDEEIDESLAAQFLN CHHHHHHHHHHHHHH		22802335
1370	Phosphorylation	LAAQFLNSGNVRHRL HHHHHHHCCCHHHEE		25619855
1400	Phosphorylation	YQAVRQFSVQAEDER HHHHHHHCCCCCCCC		21149613
1409	Phosphorylation	QAEDERESTDDESNP CCCCCCCCCCCCCCC		27087446
1410	Phosphorylation	AEDERESTDDESNPL CCCCCCCCCCCCCCC		27087446
1414	Phosphorylation	RESTDDESNPLGRAG CCCCCCCCCCCCCCC		25159016
1454	Phosphorylation	GKRGRAQTAPTKTSP CCCCCCCCCCCCCCC		26824392
1457	Phosphorylation	GRAQTAPTKTSPRNA CCCCCCCCCCCCCCH		26824392
1458	Acetylation	RAQTAPTKTSPRNAK CCCCCCCCCCCCCHH		23806337
1459	Phosphorylation	AQTAPTKTSPRNAKK CCCCCCCCCCCCHHC		26824392
1460	Phosphorylation	QTAPTKTSPRNAKKH CCCCCCCCCCCHHCC		26824392
1513	Ubiquitination	LLLRVLHAISRYWYY HHHHHHHHHHHHHHH		27667366
1540	Ubiquitination	TSEFINSKLTAKANR HHHHHHHHHHHHHHH		22790023
1543	Ubiquitination	FINSKLTAKANRQLQ HHHHHHHHHHHHHCC		27667366
1544	Ubiquitination	INSKLTAKANRQLQD HHHHHHHHHHHHCCC		27667366
1547	Ubiquitination	KLTAKANRQLQDPLV HHHHHHHHHCCCCEE		27667366
1554	Ubiquitination	RQLQDPLVIMTGNIP HHCCCCEEEEECCCH		27667366
1555	Ubiquitination	QLQDPLVIMTGNIPT HCCCCEEEEECCCHH		27667366
1560	Ubiquitination	LVIMTGNIPTWLTEL EEEEECCCHHHHHHH		27667366
1561	Ubiquitination	VIMTGNIPTWLTELG EEEECCCHHHHHHHH		27667366
1562	Ubiquitination	IMTGNIPTWLTELGK EEECCCHHHHHHHHC		27667366
1581	Ubiquitination	FFPFDTRQMLFYVTA ECCCCHHHHEEEEEE		27667366
1582	Ubiquitination	FPFDTRQMLFYVTAF CCCCHHHHEEEEEEC		27667366
1584	Ubiquitination	FDTRQMLFYVTAFDR CCHHHHEEEEEECCH		27667366
1585	Ubiquitination	DTRQMLFYVTAFDRD CHHHHEEEEEECCHH		27667366
1586	Ubiquitination	TRQMLFYVTAFDRDR HHHHEEEEEECCHHH		27667366
1588	Ubiquitination	QMLFYVTAFDRDRAM HHEEEEEECCHHHHH		27667366
1589	Ubiquitination	MLFYVTAFDRDRAMQ HEEEEEECCHHHHHH		27667366
1608	Phosphorylation	TNPEINQSDSQDSRV CCCCCCCCCCCCCCH		28066266
1610	Phosphorylation	PEINQSDSQDSRVAP CCCCCCCCCCCCHHH		21149613
1613	Phosphorylation	NQSDSQDSRVAPRLD CCCCCCCCCHHHHHC		28066266
1692	Ubiquitination	GEEVTLSNPKGSQEG CCEEECCCCCCCHHH		27667366
1694	Ubiquitination	EVTLSNPKGSQEGTK EEECCCCCCCHHHHH		22790023
1696	Phosphorylation	TLSNPKGSQEGTKYI ECCCCCCCHHHHHHH		27149854
1701	Ubiquitination	KGSQEGTKYIQNLQG CCCHHHHHHHHHHHH		22790023
1719	Acetylation	LPFGRTAKPAHIAKV CCCCCCCCCHHHHHH		7713221
1719	Ubiquitination	LPFGRTAKPAHIAKV CCCCCCCCCHHHHHH		27667366
1722	Ubiquitination	GRTAKPAHIAKVKMK CCCCCCHHHHHHHHH		27667366
1723	Ubiquitination	RTAKPAHIAKVKMKF CCCCCHHHHHHHHHH		27667366
1726	Ubiquitination	KPAHIAKVKMKFRFL CCHHHHHHHHHHHHH		27667366
1733	Ubiquitination	VKMKFRFLGKLMAKA HHHHHHHHHHHHHHH		27667366
1734	Ubiquitination	KMKFRFLGKLMAKAI HHHHHHHHHHHHHHH		27667366
1739	Ubiquitination	FLGKLMAKAIMDFRL HHHHHHHHHHHHHCC		27667366
1740	Ubiquitination	LGKLMAKAIMDFRLV HHHHHHHHHHHHCCC		27667366
1741	Ubiquitination	GKLMAKAIMDFRLVD HHHHHHHHHHHCCCC		27667366
1757	Ubiquitination	PLGLPFYKWMLRQET CCCCCHHHHHHHCCC		-
1760	Ubiquitination	LPFYKWMLRQETSLT CCHHHHHHHCCCCCC		27667366
1761	Ubiquitination	PFYKWMLRQETSLTS CHHHHHHHCCCCCCC		27667366
1763	Ubiquitination	YKWMLRQETSLTSHD HHHHHHCCCCCCCCC		27667366
1764	Ubiquitination	KWMLRQETSLTSHDL HHHHHCCCCCCCCCC		27667366
1765	Ubiquitination	WMLRQETSLTSHDLF HHHHCCCCCCCCCCC		27667366
1767	Ubiquitination	LRQETSLTSHDLFDI HHCCCCCCCCCCCCC		27667366
1768	Ubiquitination	RQETSLTSHDLFDID HCCCCCCCCCCCCCC		27667366
2010	Ubiquitination	SIDIMRDKLLIAARE CHHHHHHHHHHHHHC		22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TRIPC_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TRIPC_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRIPC_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SOX6_MOUSE	Sox6	physical	23663701

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.