dbPTM

TOPRS_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TOPRS_MOUSE
UniProt AC	Q80Z37
Protein Name	E3 ubiquitin-protein ligase Topors
Gene Name	Topors
Organism	Mus musculus (Mouse).
Sequence Length	1033
Subcellular Localization	Nucleus . Nucleus, PML body . Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage.
Protein Description	Functions as an E3 ubiquitin-protein ligase and as a E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA-damage-induced cell death through IKBKE sumoylation..
Protein Sequence	MGSQPPPPGSPLSREEGEAPPLVPAEEGRRRSRRVRLRGSCRHRPSLLSRRELASNGPAVPATASSEIMASAAKEFKMDNFSPKAGTSKLQQTVPADASPDSKCPICLDRFDNVSYLDRCLHKFCFRCVQEWSKNKAECPLCKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFTNPEVRRFRYRTTMTRERSASLYSPSSTVSRRTTTPPDSGVLFEGLGISTRPRDVDIPQFMRQMALRGPTTTDERSLRKIQEQDIINFRRTLYRAGVRVRSIEDGGRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGAHGSLVNIVQHIIMSNVTRYDLESQAFVSDLRPFLLNRTEHFIHEFISFARSPFNMAAFDQHANYDCPPSSEEGSRSDSSVITISPDEAETQELDMNASTVRQAPWDDETPGPSYSSSEQVHVGVSSLLNSSDSSDEELVSGGTTSQIQGVQTNDDVNNDSDSSSDNCVIVGFVKPLAERTPELVELSSDSEELGPYEKVETVKTQEQEQSYSSGDSDVSRASSPRSVLGKDEQMSKSHCDSDTRISSKKEEKRSTSLPAPRDSSSTRGDRVCSPYNHRHRKGGRSRSSDSRSQSRSGHDPRNHRKHGKKRLRNKRSRSRESSSRPRARKDKKRSRTRDSSWSRRSQTLSLSSGSTSRSRSRSSDHGKRRSRSRNRDRYYLRNNYGSKYKWEYTYYSRNKDRDGYESSYRRRTLSRAHYSRQSSSPEFRIQSFSERTNARKKNHSERKYYYYERRRSRSVSSNRSRTTSAGPDRVRNEKPGGKRKYKTRHLEGTSEEAQPAREFTSKGKDSHYQKSKLDGSYKNESDSFSDSRSSDRETKHKRRRRRTRSLSVEIVYEGKATDTSKHHKKKKKKHKKKHKKHHGDNTSRSPVVITIDSDSDGESEVKAGIECSNGSLPQPIQDGAFETKDVVTIEDELGVLDKDCDVTALADDLSTSQTVENCDSPAVPVEQTLDVREESTFASDLESQSSNVSIQAEPSRPVPSPRTSLSSVSPGRDCDVS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MGSQPPPPGS -----CCCCCCCCCC	51.91	26643407
10	Phosphorylation	SQPPPPGSPLSREEG CCCCCCCCCCCCCCC	28.57	26824392
13	Phosphorylation	PPPGSPLSREEGEAP CCCCCCCCCCCCCCC	40.82	25619855
55	Phosphorylation	LSRRELASNGPAVPA HHHHHHHHCCCCCCC	55.75	22807455
63	Phosphorylation	NGPAVPATASSEIMA CCCCCCCCCCHHHHH	22.47	22807455
65	Phosphorylation	PAVPATASSEIMASA CCCCCCCCHHHHHHH	24.93	22807455
66	Phosphorylation	AVPATASSEIMASAA CCCCCCCHHHHHHHH	28.78	22807455
74	Ubiquitination	EIMASAAKEFKMDNF HHHHHHHHHHCCCCC	64.50	-
82	Phosphorylation	EFKMDNFSPKAGTSK HHCCCCCCCCCCCCC	31.57	25521595
93	Phosphorylation	GTSKLQQTVPADASP CCCCCCEECCCCCCC	18.59	25619855
99	Phosphorylation	QTVPADASPDSKCPI EECCCCCCCCCCCCC	29.77	25521595
102	Phosphorylation	PADASPDSKCPICLD CCCCCCCCCCCCCCC	39.27	26824392
168	Phosphorylation	EYVLRPSYNGSFTNP HHCCCCCCCCCCCCH	26.65	30635358
171	Phosphorylation	LRPSYNGSFTNPEVR CCCCCCCCCCCHHHH	25.84	30635358
173	Phosphorylation	PSYNGSFTNPEVRRF CCCCCCCCCHHHHHH	52.11	30635358
191	Phosphorylation	TTMTRERSASLYSPS ECCCCCCCCCCCCCC	19.91	25619855
193	Phosphorylation	MTRERSASLYSPSST CCCCCCCCCCCCCCC	29.14	25619855
195	Phosphorylation	RERSASLYSPSSTVS CCCCCCCCCCCCCCC	18.95	25619855
196	Phosphorylation	ERSASLYSPSSTVSR CCCCCCCCCCCCCCC	24.49	25521595
198	Phosphorylation	SASLYSPSSTVSRRT CCCCCCCCCCCCCCC	32.47	25619855
199	Phosphorylation	ASLYSPSSTVSRRTT CCCCCCCCCCCCCCC	35.89	25619855
200	Phosphorylation	SLYSPSSTVSRRTTT CCCCCCCCCCCCCCC	27.17	25619855
202	Phosphorylation	YSPSSTVSRRTTTPP CCCCCCCCCCCCCCC	19.01	25619855
205	Phosphorylation	SSTVSRRTTTPPDSG CCCCCCCCCCCCCCC	33.63	23984901
206	Phosphorylation	STVSRRTTTPPDSGV CCCCCCCCCCCCCCC	35.18	28066266
207	Phosphorylation	TVSRRTTTPPDSGVL CCCCCCCCCCCCCCE	32.01	26643407
211	Phosphorylation	RTTTPPDSGVLFEGL CCCCCCCCCCEEECC	36.25	28066266
251	Ubiquitination	TDERSLRKIQEQDII CCHHHHHHHHHHHHH	54.77	-
335	Phosphorylation	VTRYDLESQAFVSDL CCCCCHHHHHHHHHH	33.82	-
340	Phosphorylation	LESQAFVSDLRPFLL HHHHHHHHHHHHHHH	25.19	-
492	Phosphorylation	VKPLAERTPELVELS EHHHHHCCHHHEECC	16.78	26643407
499	Phosphorylation	TPELVELSSDSEELG CHHHEECCCCCCCCC	20.70	26643407
500	Phosphorylation	PELVELSSDSEELGP HHHEECCCCCCCCCC	58.75	26643407
502	Phosphorylation	LVELSSDSEELGPYE HEECCCCCCCCCCCC	34.61	26643407
508	Phosphorylation	DSEELGPYEKVETVK CCCCCCCCCCEEECC	26.95	26643407
516	Phosphorylation	EKVETVKTQEQEQSY CCEEECCCHHHHHHH	32.76	-
538	Phosphorylation	SRASSPRSVLGKDEQ HHCCCCHHHCCCCHH	25.69	25263469
566	Phosphorylation	SKKEEKRSTSLPAPR CCHHHHHCCCCCCCC	33.16	27600695
567	Phosphorylation	KKEEKRSTSLPAPRD CHHHHHCCCCCCCCC	38.01	25521595
568	Phosphorylation	KEEKRSTSLPAPRDS HHHHHCCCCCCCCCC	33.33	22942356
576	Phosphorylation	LPAPRDSSSTRGDRV CCCCCCCCCCCCCCC	39.76	-
585	Phosphorylation	TRGDRVCSPYNHRHR CCCCCCCCCCCCCCC	27.80	25521595
587	Phosphorylation	GDRVCSPYNHRHRKG CCCCCCCCCCCCCCC	13.80	21149613
600	Phosphorylation	KGGRSRSSDSRSQSR CCCCCCCCCCCHHCC	38.20	21183079
667	Phosphorylation	LSLSSGSTSRSRSRS EECCCCCCCCCCCCC	31.52	-
668	Phosphorylation	SLSSGSTSRSRSRSS ECCCCCCCCCCCCCC	29.92	-
671	Methylation	SGSTSRSRSRSSDHG CCCCCCCCCCCCCCC	34.67	16289289
696	Phosphorylation	RYYLRNNYGSKYKWE HEEECCCCCCCCEEE	26.73	28576409
716	Phosphorylation	RNKDRDGYESSYRRR CCCCCCCCCCHHHHH	19.54	27149854
718	Phosphorylation	KDRDGYESSYRRRTL CCCCCCCCHHHHHHH	24.96	23684622
719	Phosphorylation	DRDGYESSYRRRTLS CCCCCCCHHHHHHHH	15.66	27149854
720	Phosphorylation	RDGYESSYRRRTLSR CCCCCCHHHHHHHHH	19.88	27149854
730	Phosphorylation	RTLSRAHYSRQSSSP HHHHHHHHCCCCCCC	12.45	22324799
731	Phosphorylation	TLSRAHYSRQSSSPE HHHHHHHCCCCCCCC	17.61	26643407
734	Phosphorylation	RAHYSRQSSSPEFRI HHHHCCCCCCCCHHH	31.50	26824392
735	Phosphorylation	AHYSRQSSSPEFRIQ HHHCCCCCCCCHHHH	41.39	24899341
736	Phosphorylation	HYSRQSSSPEFRIQS HHCCCCCCCCHHHHH	33.42	24899341
743	Phosphorylation	SPEFRIQSFSERTNA CCCHHHHHHHHHHCH	28.50	23984901
745	Phosphorylation	EFRIQSFSERTNARK CHHHHHHHHHHCHHH	31.26	23984901
759	Sumoylation	KKNHSERKYYYYERR HCCHHHHHHHHHHHH	33.29	28289178
759	Ubiquitination	KKNHSERKYYYYERR HCCHHHHHHHHHHHH	33.29	-
859	Phosphorylation	HKRRRRRTRSLSVEI HHHHHHHHCCCEEEE	23.85	22817900
861	Phosphorylation	RRRRRTRSLSVEIVY HHHHHHCCCEEEEEE	25.12	22817900
863	Phosphorylation	RRRTRSLSVEIVYEG HHHHCCCEEEEEECC	20.96	22817900
898	Phosphorylation	KKHHGDNTSRSPVVI HHHCCCCCCCCCEEE	30.20	22324799
899	Phosphorylation	KHHGDNTSRSPVVIT HHCCCCCCCCCEEEE	36.69	29899451
901	Phosphorylation	HGDNTSRSPVVITID CCCCCCCCCEEEEEC	23.09	23684622
906	Phosphorylation	SRSPVVITIDSDSDG CCCCEEEEECCCCCC	13.71	21149613
909	Phosphorylation	PVVITIDSDSDGESE CEEEEECCCCCCCCE	34.85	23684622
911	Phosphorylation	VITIDSDSDGESEVK EEEECCCCCCCCEEC	52.01	23684622
915	Phosphorylation	DSDSDGESEVKAGIE CCCCCCCCEECEEEE	53.90	22324799
924	Phosphorylation	VKAGIECSNGSLPQP ECEEEEECCCCCCCC	31.28	25293948
927	Phosphorylation	GIECSNGSLPQPIQD EEEECCCCCCCCCCC	40.92	25293948
976	Phosphorylation	QTVENCDSPAVPVEQ CCCCCCCCCCCCCEE	19.57	28973931
991	Phosphorylation	TLDVREESTFASDLE EECCCHHHCCCHHHH	24.87	25619855
992	Phosphorylation	LDVREESTFASDLES ECCCHHHCCCHHHHH	26.88	25619855
995	Phosphorylation	REESTFASDLESQSS CHHHCCCHHHHHCCC	38.24	25619855
999	Phosphorylation	TFASDLESQSSNVSI CCCHHHHHCCCCCEE	42.53	25619855
1001	Phosphorylation	ASDLESQSSNVSIQA CHHHHHCCCCCEEEE	32.91	25619855
1002	Phosphorylation	SDLESQSSNVSIQAE HHHHHCCCCCEEEEC	33.35	25619855
1005	Phosphorylation	ESQSSNVSIQAEPSR HHCCCCCEEEECCCC	17.15	25619855
1011	Phosphorylation	VSIQAEPSRPVPSPR CEEEECCCCCCCCCC	40.50	25619855
1016	Phosphorylation	EPSRPVPSPRTSLSS CCCCCCCCCCCCCCC	27.52	25521595
1019	Phosphorylation	RPVPSPRTSLSSVSP CCCCCCCCCCCCCCC	37.09	25619855
1020	Phosphorylation	PVPSPRTSLSSVSPG CCCCCCCCCCCCCCC	27.77	25619855
1022	Phosphorylation	PSPRTSLSSVSPGRD CCCCCCCCCCCCCCC	28.58	25619855
1022	O-linked_Glycosylation	PSPRTSLSSVSPGRD CCCCCCCCCCCCCCC	28.58	30059200
1023	Phosphorylation	SPRTSLSSVSPGRDC CCCCCCCCCCCCCCC	31.72	25619855
1025	Phosphorylation	RTSLSSVSPGRDCDV CCCCCCCCCCCCCCC	24.47	27087446
1033	Phosphorylation	PGRDCDVS------- CCCCCCCC-------	22.66	25619855

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
718	S	Phosphorylation	Kinase	PLK1	Q07832	Uniprot
1016	S	Phosphorylation	Kinase	MTOR	Q9JLN9	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
99	S	Phosphorylation	21183079
Phosphorylation at Ser-99 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity.
99	S	ubiquitylation	21183079
Phosphorylation at Ser-99 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity.
718	S	Phosphorylation	-
Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the E3 SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.
718	S	ubiquitylation	-
Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the E3 SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TOPRS_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
PRS4_MOUSE	Psmc1	physical	26872363

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TOPRS_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-911 AND SER-1025, ANDMASS SPECTROMETRY.	17242355 [Abstract]

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