TNNT2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TNNT2_HUMAN
• UniProt AC: P45379
• Protein Name: Troponin T, cardiac muscle
• Gene Name: TNNT2
• Organism: Homo sapiens (Human).
• Sequence Length: 298
• Protein Description: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity..
• Protein Sequence: MSDIEEVVEEYEEEEQEEAAVEEEEDWREDEDEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSDIEEVVE ------CCHHHHHHH	47.50	21639091
2	Acetylation	------MSDIEEVVE ------CCHHHHHHH	47.50	-
161 (in isoform 12)	Phosphorylation	-	27.26	12819028
165 (in isoform 12)	Phosphorylation	-	46.14	12819028
170 (in isoform 12)	Phosphorylation	-	65.29	2584239
189	Phosphorylation	ARKKKALSNMMHFGG HHHHHHHHHHHHHHH	27.78	-
191 (in isoform 11)	Phosphorylation	-	1.66	12819028
195 (in isoform 11)	Phosphorylation	-	17.20	12819028
197	Phosphorylation	NMMHFGGYIQKQAQT HHHHHHHHHHHHHHH	10.68	-
200 (in isoform 11)	Phosphorylation	-	37.73	2584239
201 (in isoform 10)	Phosphorylation	-	23.48	12819028
203 (in isoform 5)	Phosphorylation	-	41.21	12819028
204	Phosphorylation	YIQKQAQTERKSGKR HHHHHHHHHHHHCCC	40.55	12819028
205 (in isoform 10)	Phosphorylation	-	46.13	12819028
207 (in isoform 5)	Phosphorylation	-	58.28	12819028
208	Phosphorylation	QAQTERKSGKRQTER HHHHHHHHCCCHHHH	56.63	12819028
210 (in isoform 10)	Phosphorylation	-	48.72	2584239
213	Phosphorylation	RKSGKRQTEREKKKK HHHCCCHHHHHHHHH	40.83	2584239
249	Phosphorylation	KAKELWQSIYNLEAE HHHHHHHHHHHHHHH	18.56	-
251	Phosphorylation	KELWQSIYNLEAEKF HHHHHHHHHHHHHHC	20.95	-
290	Acetylation	KVSKTRGKAKVTGRW CCCCCCCCCCCCCCC	41.57	133991
292	Acetylation	SKTRGKAKVTGRWK- CCCCCCCCCCCCCC-	43.76	7695157
294	Phosphorylation	TRGKAKVTGRWK--- CCCCCCCCCCCC---	21.96	2584239

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
2	S	Phosphorylation	Kinase	CK2	-	Uniprot
189	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
204	T	Phosphorylation	Kinase	PRKCA	P17252	Uniprot
208	S	Phosphorylation	Kinase	PRKCA	P17252	Uniprot
213	T	Phosphorylation	Kinase	PRKCA	P17252	Uniprot
213	T	Phosphorylation	Kinase	RAF1	P04049	Uniprot
294	T	Phosphorylation	Kinase	PRKCA	P17252	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
213	T	Phosphorylation	Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TNNT2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
KDM1A_HUMAN	KDM1A	physical	23455924
MO4L2_HUMAN	MORF4L2	physical	25416956

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 115195: Cardiomyopathy, familial hypertrophic 2 (CMH2)
• 601494: Cardiomyopathy, dilated 1D (CMD1D)
• 612422: Cardiomyopathy, familial restrictive 3 (RCM3)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.