TISD_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TISD_HUMAN
• UniProt AC: P47974
• Protein Name: mRNA decay activator protein ZFP36L2 {ECO:0000305}
• Gene Name: ZFP36L2 {ECO:0000312|HGNC:HGNC:1108}
• Organism: Homo sapiens (Human).
• Sequence Length: 494
• Subcellular Localization: Nucleus . Cytoplasm . Shuttles between the nucleus and the cytoplasm in a XPO1/CRM1-dependent manner.
• Protein Description: Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. [PubMed: 25106868]
• Protein Sequence: MSTTLLSAFYDVDFLCKTEKSLANLNLNNMLDKKAVGTPVAAAPSSGFAPGFLRRHSASNLHALAHPAPSPGSCSPKFPGAANGSSCGSAAAGGPTSYGTLKEPSGGGGTALLNKENKFRDRSFSENGDRSQHLLHLQQQQKGGGGSQINSTRYKTELCRPFEESGTCKYGEKCQFAHGFHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNADERRPAPSGGASGDLRAFGTRDALHLGFPREPRPKLHHSLSFSGFPSGHHQPPGGLESPLLLDSPTSRTPPPPSCSSASSCSSSASSCSSASAASTPSGAPTCCASAAAAAAAALLYGTGGAEDLLAPGAPCAACSSASCANNAFAFGPELSSLITPLAIQTHNFAAVAAAAYYRSQQQQQQQGLAPPAQPPAPPSATLPAGAAAPPSPPFSFQLPRRLSDSPVFDAPPSPPDSLSDRDSYLSGSLSSGSLSGSESPSLDPGRRLPIFSRLSISDD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSTTLLSAF ------CCHHHHHHH	30.05	28464451
3	Phosphorylation	-----MSTTLLSAFY -----CCHHHHHHHH	21.84	28464451
4	Phosphorylation	----MSTTLLSAFYD ----CCHHHHHHHHC	20.56	27732954
7	Phosphorylation	-MSTTLLSAFYDVDF -CCHHHHHHHHCHHH	21.31	28509920
10	Phosphorylation	TTLLSAFYDVDFLCK HHHHHHHHCHHHHHC	18.12	26552605
20	Ubiquitination	DFLCKTEKSLANLNL HHHHCCCHHHHCCCC	56.87	29967540
21	Phosphorylation	FLCKTEKSLANLNLN HHHCCCHHHHCCCCC	26.87	27251275
33	Ubiquitination	NLNNMLDKKAVGTPV CCCCCCCHHCCCCCE	38.37	29967540
34	Ubiquitination	LNNMLDKKAVGTPVA CCCCCCHHCCCCCEE	48.23	29967540
38	Phosphorylation	LDKKAVGTPVAAAPS CCHHCCCCCEECCCC	14.16	25159151
45	Phosphorylation	TPVAAAPSSGFAPGF CCEECCCCCCCCCCH	38.06	23882029
46	Phosphorylation	PVAAAPSSGFAPGFL CEECCCCCCCCCCHH	36.55	23882029
57	Phosphorylation	PGFLRRHSASNLHAL CCHHHHCCCCCCHHH	31.42	27273156
59	Phosphorylation	FLRRHSASNLHALAH HHHHCCCCCCHHHCC	42.83	29255136
70	Phosphorylation	ALAHPAPSPGSCSPK HHCCCCCCCCCCCCC	43.96	23927012
73	Phosphorylation	HPAPSPGSCSPKFPG CCCCCCCCCCCCCCC	18.14	23927012
75	Phosphorylation	APSPGSCSPKFPGAA CCCCCCCCCCCCCCC	32.59	23927012
96	Phosphorylation	SAAAGGPTSYGTLKE CCCCCCCCCCCCCCC	37.54	-
98	Phosphorylation	AAGGPTSYGTLKEPS CCCCCCCCCCCCCCC	19.33	24905233
100	Phosphorylation	GGPTSYGTLKEPSGG CCCCCCCCCCCCCCC	26.82	-
102	Ubiquitination	PTSYGTLKEPSGGGG CCCCCCCCCCCCCCC	68.37	-
105	Phosphorylation	YGTLKEPSGGGGTAL CCCCCCCCCCCCCCC	51.68	23532336
115	Ubiquitination	GGTALLNKENKFRDR CCCCCCCCCCCCCCC	63.73	29967540
118	Ubiquitination	ALLNKENKFRDRSFS CCCCCCCCCCCCCCC	42.75	29967540
123	Phosphorylation	ENKFRDRSFSENGDR CCCCCCCCCCCCCCH	37.10	23927012
125	Phosphorylation	KFRDRSFSENGDRSQ CCCCCCCCCCCCHHH	31.25	29255136
131	Phosphorylation	FSENGDRSQHLLHLQ CCCCCCHHHHHHHHH	27.05	23927012
142	Ubiquitination	LHLQQQQKGGGGSQI HHHHHHHCCCCCCCC	56.00	21963094
147	Phosphorylation	QQKGGGGSQINSTRY HHCCCCCCCCCCCCC	31.03	-
155	Ubiquitination	QINSTRYKTELCRPF CCCCCCCCEEECCCC	32.03	29967540
169	Ubiquitination	FEESGTCKYGEKCQF CCCCCCCCCCCCCCC	57.01	29967540
173	Ubiquitination	GTCKYGEKCQFAHGF CCCCCCCCCCCCCCH	29.04	29967540
193	Ubiquitination	LTRHPKYKTELCRTF HHCCHHHHHHHHHHH	41.47	-
199	Phosphorylation	YKTELCRTFHTIGFC HHHHHHHHHCCCCCC	20.83	-
202	Phosphorylation	ELCRTFHTIGFCPYG HHHHHHCCCCCCCCC	20.71	-
208	Phosphorylation	HTIGFCPYGPRCHFI CCCCCCCCCCCCEEE	40.73	28152594
211	Methylation	GFCPYGPRCHFIHNA CCCCCCCCCEEECCC	23.37	-
226	Phosphorylation	DERRPAPSGGASGDL CCCCCCCCCCCCCCH	52.55	26657352
230	Phosphorylation	PAPSGGASGDLRAFG CCCCCCCCCCHHHHC	35.79	28450419
238	Phosphorylation	GDLRAFGTRDALHLG CCHHHHCCCCCHHCC	20.94	23186163
257	Phosphorylation	PRPKLHHSLSFSGFP CCCCCCCCEECCCCC	18.24	23401153
259	Phosphorylation	PKLHHSLSFSGFPSG CCCCCCEECCCCCCC	22.00	23401153
261	Phosphorylation	LHHSLSFSGFPSGHH CCCCEECCCCCCCCC	35.83	29255136
265	Phosphorylation	LSFSGFPSGHHQPPG EECCCCCCCCCCCCC	48.80	29255136
276	Phosphorylation	QPPGGLESPLLLDSP CCCCCCCCCEECCCC	26.37	28450419
282	Phosphorylation	ESPLLLDSPTSRTPP CCCEECCCCCCCCCC	30.01	29743597
284	Phosphorylation	PLLLDSPTSRTPPPP CEECCCCCCCCCCCC	34.49	23927012
285	Phosphorylation	LLLDSPTSRTPPPPS EECCCCCCCCCCCCC	37.23	23927012
394	Phosphorylation	AAAAYYRSQQQQQQQ HHHHHHHHHHHHHHC	19.07	26074081
414	Phosphorylation	AQPPAPPSATLPAGA CCCCCCCCCCCCCCC	32.59	25850435
416	Phosphorylation	PPAPPSATLPAGAAA CCCCCCCCCCCCCCC	37.30	25850435
426	Phosphorylation	AGAAAPPSPPFSFQL CCCCCCCCCCCCCCC	44.11	28348404
430	Phosphorylation	APPSPPFSFQLPRRL CCCCCCCCCCCCCCC	20.18	25850435
438	Phosphorylation	FQLPRRLSDSPVFDA CCCCCCCCCCCCCCC	33.94	23401153
440	Phosphorylation	LPRRLSDSPVFDAPP CCCCCCCCCCCCCCC	21.42	29255136
448	Phosphorylation	PVFDAPPSPPDSLSD CCCCCCCCCCCCCCC	48.26	29255136
452	Phosphorylation	APPSPPDSLSDRDSY CCCCCCCCCCCCCHH	35.23	23663014
454	Phosphorylation	PSPPDSLSDRDSYLS CCCCCCCCCCCHHHC	34.25	23663014
458	Phosphorylation	DSLSDRDSYLSGSLS CCCCCCCHHHCCCCC	28.94	26471730
461	Phosphorylation	SDRDSYLSGSLSSGS CCCCHHHCCCCCCCC	20.36	26471730
463	Phosphorylation	RDSYLSGSLSSGSLS CCHHHCCCCCCCCCC	23.00	30576142
465	Phosphorylation	SYLSGSLSSGSLSGS HHHCCCCCCCCCCCC	34.06	26471730
466	Phosphorylation	YLSGSLSSGSLSGSE HHCCCCCCCCCCCCC	37.38	26471730
468	Phosphorylation	SGSLSSGSLSGSESP CCCCCCCCCCCCCCC	22.70	26471730
470	Phosphorylation	SLSSGSLSGSESPSL CCCCCCCCCCCCCCC	41.80	25921289
472	Phosphorylation	SSGSLSGSESPSLDP CCCCCCCCCCCCCCC	31.37	24719451
474	Phosphorylation	GSLSGSESPSLDPGR CCCCCCCCCCCCCCC	22.83	26074081
476	Phosphorylation	LSGSESPSLDPGRRL CCCCCCCCCCCCCCC	55.47	26074081
487	Phosphorylation	GRRLPIFSRLSISDD CCCCCCCEEEECCCC	32.79	23927012
490	Phosphorylation	LPIFSRLSISDD--- CCCCEEEECCCC---	20.95	23927012
492	Phosphorylation	IFSRLSISDD----- CCEEEECCCC-----	32.14	30266825

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
490	S	Phosphorylation	Kinase	RPS6KA1	Q15418	Uniprot
492	S	Phosphorylation	Kinase	RPS6KA1	Q15418	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
490	S	Phosphorylation	Phosphorylated by RPS6KA1 at Ser-490 and Ser-492 upon phorbol 12-myristate 13-acetate (PMA) treatment; this phosphorylation results in dissociation of the CCR4-NOT-deadenylase complex and induces p38 MAPK-mediated stabilization of the low-density lipoprotein (LDL) receptor (LDLR) mRNA.	25106868
492	S	Phosphorylation	Phosphorylated by RPS6KA1 at Ser-490 and Ser-492 upon phorbol 12-myristate 13-acetate (PMA) treatment; this phosphorylation results in dissociation of the CCR4-NOT-deadenylase complex and induces p38 MAPK-mediated stabilization of the low-density lipoprotein (LDL) receptor (LDLR) mRNA.	25106868

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TISD_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CBY1_HUMAN	CBY1	physical	27173435

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.