dbPTM

TGT_DROME - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TGT_DROME
UniProt AC	Q9VPY8
Protein Name	Queuine tRNA-ribosyltransferase catalytic subunit {ECO:0000255\|HAMAP-Rule:MF_03218}
Gene Name	Tgt
Organism	Drosophila melanogaster (Fruit fly).
Sequence Length	427
Subcellular Localization	Cytoplasm .
Protein Description	Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product..
Protein Sequence	MGPSHIPPLTYKVVAECSVSKARAGLMTLRHSEVNTPVFMPVGTQGTLKGIVPDQLIELNCQILLGNTYHLGLRPGIETLKKAGGLHKFMGWPRAILTDSGGFQMVSLLQLAEIDEHGVNFRSPFDNSQCMLTPEHSIEIQNAIGGDIMMQLDDVVKTTTTGPRVEEAMERTIRWVDRCIEAHARDDDQSLFPIVQGGLDVPLRQRCVSALMERQVRGFAVGGLSGGESKHDFWRMVDVCTGYLPKDKPRYLMGVGFAADLVVCVALGIDMFDCVFPTRTARFGCALVDSGQLNLKQPKYKLDMEPIDKDCDCSTCRRYTRSYLHHIATNESVSSSLLSIHNVAYQLRLMRSMREAIQRDEFPQFVADFMARHFKAEPVPAWIREALSAVNIQLPADPERIDEQDQKPKTEKRRETEDVAEEQVASS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference	Orthologous Protein Cluster
416	Phosphorylation	KTEKRRETEDVAEEQ CCHHHHHHHHHHHHH	35.36	19429919

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of TGT_DROME !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of TGT_DROME !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of TGT_DROME !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
QTRT2_DROME	CG3434	physical	22036573

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TGT_DROME

Related Literatures of Post-Translational Modification