TEX14_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TEX14_HUMAN
• UniProt AC: Q8IWB6
• Protein Name: Inactive serine/threonine-protein kinase TEX14
• Gene Name: TEX14
• Organism: Homo sapiens (Human).
• Sequence Length: 1497
• Subcellular Localization: Cytoplasm. Midbody. Chromosome, centromere, kinetochore. Detected in the intercellular bridges that connect male germ cell daughter cells after cell division..
• Protein Description: Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity)..
• Protein Sequence: MSRAVRLPVPCPVQLGTLRNDSLEAQLHEYVKQGNYVKVKKILKKGIYVDAVNSLGQTALFVAALLGLRKFVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLDAGGDLRLHDERGQNPKTWALTAGKERSTQIVEFMQRCASHMQAIIQGFSYDLLKKIDSPQRLVYSPSWCGGLVQGNPNGSPNRLLKAGVISAQNIYSFGFGKAMPWFQFYLTGATQMAYLGSLPVIGEKEVIQADDEPTFSFFSGPYMVMTNLVWNGSRVTVKELNLPTHPHCSRLRLADLLIAEQEHSSKLRHPYLLQLMAVCLSQDLEKTRLVYERITIGTLFSVLHERRSQFPVLHMEVIVHLLLQISDALRYLHFQGFIHRSLSSYAVHIISPGEARLTNLEYMLESEDRGVQRDLTRVPLPTQLYNWAAPEVILQKAATVKSDIYSFSMIMQEILTDDIPWKGLDGSVVKKAVVSGNYLEADVRLPKPYYDIVKSGIHVKQKDRTMNLQDIRYILKNDLKDFTGAQRTQPTESPRVQRYGLHPDVNVYLGLTSEHPRETPDMEIIELKEMGSQPHSPRVHSLFTEGTLDPQAPDPCLMARETQNQDAPCPAPFMAEEASSPSTGQPSLCSFEINEIYSGCLILEDDIEEPPGAASSLEADGPNQVDELKSMEEELDKMEREACCFGSEDESSSKAETEYSFDDWDWQNGSLSSLSLPESTREAKSNLNNMSTTEEYLISKCVLDLKIMQTIMHENDDRLRNIEQILDEVEMKQKEQEERMSLWATSREFTNAYKLPLAVGPPSLNYIPPVLQLSGGQKPDTSGNYPTLPRFPRMLPTLCDPGKQNTDEQFQCTQGAKDSLETSRIQNTSSQGRPRESTAQAKATQFNSALFTLSSHRQGPSASPSCHWDSTRMSVEPVSSEIYNAESRNKDDGKVHLKWKMEVKEMAKKAATGQLTVPPWHPQSSLTLESEAENEPDALLQPPIRSPENTDWQRVIEYHRENDEPRGNGKFDKTGNNDCDSDQHGRQPRLGSFTSIRHPSPRQKEQPEHSEAFQASSDTLVAVEKSYSHQSMQSTCSPESSEDITDEFLTPDGEYFYSSTAQENLALETSSPIEEDFEGIQGAFAQPQVSGEEKFQMRKILGKNAEILPRSQFQPVRSTEDEQEETSKESPKELKEKDISLTDIQDLSSISYEPDSSFKEASCKTPKINHAPTSVSTPLSPGSVSSAASQYKDCLESITFQVKTEFASCWNSQEFIQTLSDDFISVRERAKKLDSLLTSSETPPSRLTGLKRLSSFIGAGSPSLVKACDSSPPHATQRRSLPKVEAFSQHHIDELPPPSQELLDDIELLKQQQGSSTVLHENTASDGGGTANDQRHLEEQETDSKKEDSSMLLSKETEDLGEDTERAHSTLDEDLERWLQPPEESVELQDLPKGSERETNIKDQKVGEEKRKREDSITPERRKSEGVLGTSEEDELKSCFWKRLGWSESSRIIVLDQSDLSD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
36	Phosphorylation	EYVKQGNYVKVKKIL HHHHCCCEEEHHHHH	14.42	-
168	Phosphorylation	DLLKKIDSPQRLVYS HHHHCCCCCCEEEEC	26.80	28555341
174	Phosphorylation	DSPQRLVYSPSWCGG CCCCEEEECCCCCCC	21.32	27307780
175	Phosphorylation	SPQRLVYSPSWCGGL CCCEEEECCCCCCCH	12.65	27307780
177	Phosphorylation	QRLVYSPSWCGGLVQ CEEEECCCCCCCHHC	29.11	27307780
206	Phosphorylation	VISAQNIYSFGFGKA EEEEEEEEECCCCCC	12.90	-
284	Phosphorylation	LPTHPHCSRLRLADL CCCCCCHHHHHHHHH	31.17	-
437	Phosphorylation	QKAATVKSDIYSFSM HHCCCCCHHHHHHHH	25.55	22405274
508	Phosphorylation	MNLQDIRYILKNDLK CCHHHHHHHHHHCHH	15.78	22817900
511	Acetylation	QDIRYILKNDLKDFT HHHHHHHHHCHHCCC	38.57	133703
515	Acetylation	YILKNDLKDFTGAQR HHHHHCHHCCCCCCC	54.37	133699
554	Phosphorylation	TSEHPRETPDMEIIE CCCCCCCCCCCEEEE	26.35	25690035
567	Phosphorylation	IELKEMGSQPHSPRV EEHHHCCCCCCCCCC	38.95	-
571	Phosphorylation	EMGSQPHSPRVHSLF HCCCCCCCCCCHHCC	22.53	23403867
618	Phosphorylation	EEASSPSTGQPSLCS CCCCCCCCCCCCCCC	43.13	22405274
665	Phosphorylation	NQVDELKSMEEELDK CHHHHHHHHHHHHHH	44.01	-
687	Phosphorylation	FGSEDESSSKAETEY CCCCCCCCCCCCCEE	33.53	22468782
688	Phosphorylation	GSEDESSSKAETEYS CCCCCCCCCCCCEEE	45.20	22468782
707	Phosphorylation	DWQNGSLSSLSLPES CCCCCCCCCCCCCHH	30.81	22468782
727	Phosphorylation	SNLNNMSTTEEYLIS HHHCCCCCHHHHHHH	27.71	22405274
728	Phosphorylation	NLNNMSTTEEYLISK HHCCCCCHHHHHHHH	21.36	22405274
776	Phosphorylation	KEQEERMSLWATSRE HHHHHHHHHHHHCHH	27.29	29083192
780	Phosphorylation	ERMSLWATSREFTNA HHHHHHHHCHHHHHH	19.41	29083192
781	Phosphorylation	RMSLWATSREFTNAY HHHHHHHCHHHHHHC	23.28	29083192
785	Phosphorylation	WATSREFTNAYKLPL HHHCHHHHHHCCCCC	17.74	29083192
788	Phosphorylation	SREFTNAYKLPLAVG CHHHHHHCCCCCCCC	18.35	29083192
863	Phosphorylation	ETSRIQNTSSQGRPR HHHCCCCCCCCCCCC	17.24	29978859
864	Phosphorylation	TSRIQNTSSQGRPRE HHCCCCCCCCCCCCC	28.37	29978859
865	Phosphorylation	SRIQNTSSQGRPRES HCCCCCCCCCCCCCC	34.32	29978859
873	Phosphorylation	QGRPRESTAQAKATQ CCCCCCCHHHHHHHH	20.31	22210691
877	Acetylation	RESTAQAKATQFNSA CCCHHHHHHHHHHHH	39.63	7925333
915	Phosphorylation	MSVEPVSSEIYNAES CCEECCCHHHCCCCC	28.40	-
923	Ubiquitination	EIYNAESRNKDDGKV HHCCCCCCCCCCCCE	45.80	30230243
925	Acetylation	YNAESRNKDDGKVHL CCCCCCCCCCCCEEE	56.96	19815641
929	Ubiquitination	SRNKDDGKVHLKWKM CCCCCCCCEEEEEEH	33.21	30230243
935	Sumoylation	GKVHLKWKMEVKEMA CCEEEEEEHHHHHHH	24.48	-
935	Acetylation	GKVHLKWKMEVKEMA CCEEEEEEHHHHHHH	24.48	7429667
935	Sumoylation	GKVHLKWKMEVKEMA CCEEEEEEHHHHHHH	24.48	-
1027	Phosphorylation	GRQPRLGSFTSIRHP CCCCCCCCCCCCCCC	29.91	29449344
1029	Phosphorylation	QPRLGSFTSIRHPSP CCCCCCCCCCCCCCC	25.58	29449344
1030	Phosphorylation	PRLGSFTSIRHPSPR CCCCCCCCCCCCCCC	18.67	29496963
1035	Phosphorylation	FTSIRHPSPRQKEQP CCCCCCCCCCCCCCC	26.96	17081983
1106	Phosphorylation	NLALETSSPIEEDFE CEEECCCCCCHHHCC	37.00	-
1187	Phosphorylation	QDLSSISYEPDSSFK HHHHHCCCCCCCCCC	29.79	-
1215	Phosphorylation	TSVSTPLSPGSVSSA CCCCCCCCCCCHHHH	28.55	-
1226	Phosphorylation	VSSAASQYKDCLESI HHHHHHHHHHHHHHC	13.29	-
1277	Phosphorylation	SLLTSSETPPSRLTG HHHHCCCCCHHHHHH	42.31	22817900
1280	Phosphorylation	TSSETPPSRLTGLKR HCCCCCHHHHHHHHH	40.92	22817900
1289	Phosphorylation	LTGLKRLSSFIGAGS HHHHHHHHHHHCCCC	27.51	28857561
1290	Phosphorylation	TGLKRLSSFIGAGSP HHHHHHHHHHCCCCH	26.02	28857561
1305	Phosphorylation	SLVKACDSSPPHATQ HHHHHCCCCCCCHHC	44.63	-
1384	Phosphorylation	TDSKKEDSSMLLSKE CCCHHHHHHHHHCHH	20.81	-
1385	Phosphorylation	DSKKEDSSMLLSKET CCHHHHHHHHHCHHH	26.27	-
1389	Phosphorylation	EDSSMLLSKETEDLG HHHHHHHCHHHHCCC	25.20	29457462
1404	Phosphorylation	EDTERAHSTLDEDLE CCCHHHHHCCCHHHH	29.67	-
1459	Phosphorylation	ITPERRKSEGVLGTS CCHHHHHCCCCCCCC	37.46	-
1496	Phosphorylation	VLDQSDLSD------ EEEHHHCCC------	46.38	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
437	S	Phosphorylation	Kinase	PLK1	P53350	Uniprot
618	T	Phosphorylation	Kinase	CDK1	P06493	PSP
727	T	Phosphorylation	Kinase	CDK1	P06493	PSP
728	T	Phosphorylation	Kinase	CDK1	P06493	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
437	S	Phosphorylation	Phosphorylated on Ser-437 by PLK1 during late prometaphase promotes the rapid depletion from kinetochores and its subsequent degradation by the APC/C complex.	22405274

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TEX14_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CEP55_HUMAN	CEP55	physical	20176808
TEX14_HUMAN	TEX14	physical	20176808

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.