STRAA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: STRAA_HUMAN
• UniProt AC: Q7RTN6
• Protein Name: STE20-related kinase adapter protein alpha
• Gene Name: STRADA
• Organism: Homo sapiens (Human).
• Sequence Length: 431
• Subcellular Localization: Nucleus . Cytoplasm .
• Protein Description: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation..
• Protein Sequence: MSFLVSKPERIRRWVSEKFIVEGLRDLELFGEQPPGDTRRKTNDASSESIASFSKQEVMSSFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARPSASTLLNHSFFKQIKRRASEALPELLRPVTPITNFEGSQSQDHSGIFGLVTNLEELEVDDWEF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2 (in isoform 2)	Phosphorylation	-	27.56	25693802
2 (in isoform 3)	Phosphorylation	-	27.56	25693802
2	Phosphorylation	------MSFLVSKPE ------CCCCCCCHH	27.56	25850435
5 (in isoform 2)	Phosphorylation	-	6.96	25693802
5 (in isoform 3)	Phosphorylation	-	6.96	25693802
9 (in isoform 3)	Phosphorylation	-	65.56	28348404
9 (in isoform 2)	Phosphorylation	-	65.56	28348404
9	Phosphorylation	SFLVSKPERIRRWVS CCCCCCHHHHHHHHC	65.56	27251275
10 (in isoform 2)	Phosphorylation	-	35.92	28348404
10 (in isoform 3)	Phosphorylation	-	35.92	28348404
12 (in isoform 2)	Phosphorylation	-	32.41	25693802
12 (in isoform 3)	Phosphorylation	-	32.41	25693802
13 (in isoform 6)	Phosphorylation	-	35.46	29523821
15 (in isoform 2)	Phosphorylation	-	5.13	25693802
15 (in isoform 3)	Phosphorylation	-	5.13	25693802
17 (in isoform 2)	Phosphorylation	-	42.22	25693802
17 (in isoform 6)	Phosphorylation	-	42.22	25849741
17 (in isoform 3)	Phosphorylation	-	42.22	25693802
18 (in isoform 6)	Phosphorylation	-	32.65	25849741
20 (in isoform 6)	Phosphorylation	-	4.00	25849741
23 (in isoform 6)	Phosphorylation	-	18.34	25849741
25 (in isoform 6)	Phosphorylation	-	58.27	29523821
42	Phosphorylation	PGDTRRKTNDASSES CCCCCCCCCCCCHHH	36.20	23927012
46	Phosphorylation	RRKTNDASSESIASF CCCCCCCCHHHHHHC	37.19	23927012
47	Phosphorylation	RKTNDASSESIASFS CCCCCCCHHHHHHCC	36.76	28985074
49	Phosphorylation	TNDASSESIASFSKQ CCCCCHHHHHHCCHH	25.96	20363803
52	Phosphorylation	ASSESIASFSKQEVM CCHHHHHHCCHHHHH	28.51	23927012
54	Phosphorylation	SESIASFSKQEVMSS HHHHHHCCHHHHHHH	30.82	23927012
69	Phosphorylation	FLPEGGCYELLTVIG CCCCCCHHHHEEHHC	16.94	27050516
91	Ubiquitination	TVNLARYKPTGEYVT EEEECCCCCCCCEEE	30.46	-
202 (in isoform 3)	Ubiquitination	-	1.68	21906983
202 (in isoform 2)	Ubiquitination	-	1.68	21906983
212	Phosphorylation	VDGKVYLSGLRSNLS ECCEEEEHHHHHCCC	20.35	24719451
222	Phosphorylation	RSNLSMISHGQRQRV HHCCCEECCCCCCCE	16.96	22210691
239	Ubiquitination	DFPKYSVKVLPWLSP CCCCCCEEEHHCCCH	31.92	2190698
239 (in isoform 1)	Ubiquitination	-	31.92	21906983
302	Phosphorylation	TVPCLLDTSTIPAEE CCCEEEECCCCCHHH	27.95	28102081
303	Phosphorylation	VPCLLDTSTIPAEEL CCEEEECCCCCHHHC	24.81	28348404
304	Phosphorylation	PCLLDTSTIPAEELT CEEEECCCCCHHHCC	32.83	28348404
311	Phosphorylation	TIPAEELTMSPSRSV CCCHHHCCCCCCHHH	20.91	22199227
313	Phosphorylation	PAEELTMSPSRSVAN CHHHCCCCCCHHHHC	17.94	22199227
315	Phosphorylation	EELTMSPSRSVANSG HHCCCCCCHHHHCCC	29.85	28102081
324	Phosphorylation	SVANSGLSDSLTTST HHHCCCCCCCCCCCC	28.78	26471730
326	Phosphorylation	ANSGLSDSLTTSTPR HCCCCCCCCCCCCCC	25.00	26657352
328	Phosphorylation	SGLSDSLTTSTPRPS CCCCCCCCCCCCCCC	23.74	25850435
329	Phosphorylation	GLSDSLTTSTPRPSN CCCCCCCCCCCCCCC	35.58	22817900
330	Phosphorylation	LSDSLTTSTPRPSNG CCCCCCCCCCCCCCC	31.04	26471730
387	Phosphorylation	KQIKRRASEALPELL HHHHHHHHHHHHHHH	22.91	28111955
398	Phosphorylation	PELLRPVTPITNFEG HHHHCCCCCCCCCCC	16.20	24275569
419	Phosphorylation	SGIFGLVTNLEELEV CCEEEEECCHHHCCC	38.85	14702039

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
329	T	Phosphorylation	Kinase	STK11	Q15831	GPS
419	T	Phosphorylation	Kinase	STK11	Q15831	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of STRAA_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of STRAA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HS90A_HUMAN	HSP90AA1	physical	21860411
STK11_HUMAN	STK11	physical	21860411

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46, AND MASSSPECTROMETRY.
PubMed: 19369195

"Activation of the tumour suppressor kinase LKB1 by the STE20-likepseudokinase STRAD.";
Baas A.F., Boudeau J., Sapkota G.P., Smit L., Medema R., Morrice N.A.,Alessi D.R., Clevers H.C.;
EMBO J. 22:3062-3072(2003).
Cited for: IDENTIFICATION (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,INTERACTION WITH STK11/LKB1, MUTAGENESIS OF THR-329 AND THR-419, ANDPHOSPHORYLATION AT THR-329 AND THR-419.
PubMed: 12805220