SP3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SP3_MOUSE
• UniProt AC: O70494
• Protein Name: Transcription factor Sp3
• Gene Name: Sp3
• Organism: Mus musculus (Mouse).
• Sequence Length: 783
• Subcellular Localization: Nucleus. Nucleus, PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies (By similarity)..
• Protein Description: Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping (By similarity)..
• Protein Sequence: MTAPEKPVKQEEMAALDVDGGGGGGGHGEYLQQQQQQQQQHGNGAAAAAAQDTQPSPLALLAATCSKIGPPSPGDDDEEAAVAAAAGVPAAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPGAATSSGQYVLPLQNLQNQQIFSVAPGSDSSNGTVSNVQYQVIPQIQSTDAQQVQIGFTGSSDNGGINQENSQIQIIPGSNQTLLASGTPPANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLSGSSQTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDVNETNADTDLFVPTSSSSQLPVTIDSTGILQQNTNSLTTTSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQDSQQPTSQAQIVQGITPQTIHGVQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQITWQTFQVQGVQNLQNLQIQNTAAQQITLTPVQTLTLGQVAAGGALTSTPVSLSTGQLPNLQTVTVNSIDSTGIQLHPGENADSPADIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKVIHSSSTVLASVEAGRDDALITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQLVTVSGNETME

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Acetylation	--MTAPEKPVKQEEM --CCCCCCCCCHHHC	54.79	22902405
72	Phosphorylation	CSKIGPPSPGDDDEE HHHCCCCCCCCCHHH	44.65	27087446
546	Phosphorylation	HPGENADSPADIRIK CCCCCCCCCCCEEEC	21.41	21082442
553	Acetylation	SPADIRIKEEEPDPE CCCCEEECCCCCCHH	49.58	15554904
565	Phosphorylation	DPEEWQLSGDSTLNT CHHHHCCCCCCCCCC	26.00	21149613
568	Phosphorylation	EWQLSGDSTLNTNDL HHCCCCCCCCCCCCC	38.02	26643407
569	Phosphorylation	WQLSGDSTLNTNDLT HCCCCCCCCCCCCCC	28.85	26643407
572	Phosphorylation	SGDSTLNTNDLTHLR CCCCCCCCCCCCEEE	32.97	26643407
576	Phosphorylation	TLNTNDLTHLRVQVV CCCCCCCCEEEEEEE	23.02	26643407
595	Acetylation	DQQHQEGKRLRRVAC HHHHHHCCEEEEEEE	47.72	7662983
648	Phosphorylation	RAHLRWHSGERPFIC HHHHHHCCCCCCEEE	35.08	26643407
667	Phosphorylation	CGKRFTRSDELQRHR ECCCCCCCHHHHHHH	32.17	29514104
675	Methylation	DELQRHRRTHTGEKK HHHHHHHHCCCCCCE	25.41	16287701
691	Methylation	VCPECSKRFMRSDHL ECCHHHHHHHCHHHH	17.82	16287709

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SP3_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
553	K	Acetylation	Alternate sumoylation and acetylation at Lys-553 also control transcriptional activity (By similarity).	-
553	K	Sumoylation	Alternate sumoylation and acetylation at Lys-553 also control transcriptional activity (By similarity).	-
553	K	Sumoylation	Sumoylated on 2 sites in longer isoforms with Lys-553 being the major site.	-
553	K	Sumoylation	Sumoylation on Lys-553 represses the transactivation activity, except for the largest isoform which has little effect on transactivation.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SP3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
REST_MOUSE	Rest	physical	17130167
SIN3A_MOUSE	Sin3a	physical	16109738
HDAC2_MOUSE	Hdac2	physical	16109738

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Sp3 is involved in the regulation of SOCS3 gene expression.";
Ehlting C., Haussinger D., Bode J.G.;
Biochem. J. 387:737-745(2005).
Cited for: ACETYLATION AT LYS-553, AND FUNCTION.
PubMed: 15554904