SMO_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SMO_HUMAN
• UniProt AC: Q99835
• Protein Name: Smoothened homolog
• Gene Name: SMO
• Organism: Homo sapiens (Human).
• Sequence Length: 787
• Subcellular Localization: Membrane ; Multi-pass membrane protein . Cell projection, cilium .
• Protein Description: G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. [PubMed: 19592253 Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity]
• Protein Sequence: MAAARPARGPELPLLGLLLLLLLGDPGRGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRLGEPTSNETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKQPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGQSDDEPKRIKKSKMIAKAFSKRHELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPVPPEEQANLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
32	Phosphorylation	DPGRGAASSGNATGP CCCCCCCCCCCCCCC	37.94	-
33	Phosphorylation	PGRGAASSGNATGPG CCCCCCCCCCCCCCC	31.19	23532336
35	N-linked_Glycosylation	RGAASSGNATGPGPR CCCCCCCCCCCCCCC	35.96	UniProtKB CARBOHYD
43	Phosphorylation	ATGPGPRSAGGSARR CCCCCCCCCCCCCCC	34.11	23532336
47	Phosphorylation	GPRSAGGSARRSAAV CCCCCCCCCCCCCCC	20.06	-
55	Phosphorylation	ARRSAAVTGPPPPLS CCCCCCCCCCCCCCC	38.96	26503892
55	O-linked_Glycosylation	ARRSAAVTGPPPPLS CCCCCCCCCCCCCCC	38.96	OGP
62	Phosphorylation	TGPPPPLSHCGRAAP CCCCCCCCCCCCCCC	23.62	26503892
95	Cholesterylation	TSTLLAGDSDSQEEA HEEEECCCCCCHHHH	43.60	28344083
188	N-linked_Glycosylation	EVQNIKFNSSGQCEV CCEEEEECCCCCEEE	30.20	UniProtKB CARBOHYD
309	N-linked_Glycosylation	RLGEPTSNETLSCVI ECCCCCCCHHHHHHH	49.07	UniProtKB CARBOHYD
428	Phosphorylation	RGVMTLFSIKSNHPG HHHHHHHHHHCCCCC	32.02	24719451
438	Phosphorylation	SNHPGLLSEKAASKI CCCCCCCCHHHHHHH	41.93	24719451
500	O-linked_Glycosylation	NVTIGLPTKQPIPDC EEEECCCCCCCCCCC	47.56	OGP
662	Phosphorylation	WLVEAEISPELQKRL EEEEEECCHHHHHHH	12.11	28348404
674	Acetylation	KRLGRKKKRRKRKKE HHHCCHHHHHHHHCC	61.54	7851909
724	Phosphorylation	GAWGAGDSCRQGAWT CCCCCCCCHHCCCEE	15.41	29759185
771	Phosphorylation	QGLGPIHSRTNLMDT CCCCCCCCCCCCCCC	41.33	29759185
785	Phosphorylation	TELMDADSDF----- CHHCCCCCCC-----	42.51	22617229

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of SMO_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of SMO_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SMO_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PTC1_HUMAN	PTCH1	physical	11278759
NEDD4_HUMAN	NEDD4	physical	23106887

Drug and Disease Associations

• Kegg Disease
• H00039: Basal cell carcinoma
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• D09992: Vismodegib (USAN/INN); Erivedge (TN)
• D10119: Erismodegib (USAN); Sonidegib (INN)
• D10324: Saridegib (USAN)
• D10325: Saridegib hydrochloride (USAN)
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-785, AND MASSSPECTROMETRY.
PubMed: 19690332