RPA1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RPA1_MOUSE
• UniProt AC: O35134
• Protein Name: DNA-directed RNA polymerase I subunit RPA1
• Gene Name: Polr1a
• Organism: Mus musculus (Mouse).
• Sequence Length: 1717
• Subcellular Localization: Nucleus, nucleolus.
• Protein Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity)..
• Protein Sequence: MLASKHTPWRRLQGISFGMYSAEELKKLSVKSITNPRYVDYLGNPSANGLYDLALGPADSKEVCATCVQDFNNCSGHLGHIDLPLTVYNPFLFDKLYLLLRGSCLSCHMLTCPRAAIYLLISQLRVLEVGALQAVYELERILSRFLEETGDPSAFEIQEELEEYTSKILQNNLLGSQGTHVKNVCESRSKLVAQFWKTHMAAKQCPHCKTGRSVVRKEHNSKLIITYPATVHKKSDQEGTELPEGVPEAPGIDKAQMGKRGYLTPSSAQEHLFAIWKNEGFFLNYLFSGLDDIGPESSFNPSMFFLDFIVVPPSRYRPVNRLGDQMFTNGQTVNLQAVMKDAVLIRKLLALMAQEQKLPCEMTELTIDKENDSSVAIDRSFLGLLPGPSLTDKLYNIWIRLQSHVNIVFDSEMDKLMLEKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSSVDAAQREAVAKQLLTPATGAPKPQGTKVVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGANMTIRGCFFTREQYMELVYRGLTDKVGRVKLFPPAILKPFPLWTGKQVVSTLLINIIPEDYAPLNLSGKAKIGSKAWVKEKPRPIPDFDPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGRVLTCLARLFTAYLQLYRGFTLGVEDILVKPNADVVRQRIIEESTQCGPQAVKAALSLPETASCDEIQGKWQDAHLSKDQRDFNMIDMKFKEEVNHYSNEINKACMPLGLHRQFPENNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIRPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVIQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLAGNYEVIMKSKHLHEVLSRADPQKVLGHIKAIKKWHHKHSGALLRKGAFLSFSQKIQAAVKALNLKGSIQNGRSPETQQMLQMWYDLDEESRWKYQKRAAPCPDPSLSVWRPDIYFASVSETFEKKIDDFSQEWAAQAERSYKKSELSLDRLRTLLQLKWQRSLCDPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVFDTKKALKKVKSLKKRLTRVCLGEVLQKVDIQESFCMGEKRNKFQVYELRFQFLPHAYYQQEKCLRPEDILHFMETRFFKLLMEAIKKKKNKASAFRNVNSRRATQKDLNDTEDSGRSQREEERDEEEEGNIVDAEAEEGDADASDTKRKEKQEEEVDYESEEEGEEEEEEEVQEEGNIKGDGVHQGHEPDEEEHLGLEEEESSQKPPRRHSRPQGAEAIKRRIQAVRESYSFIEDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHKAIVYTTKGITRCLLNETTNSKNEKELVLNTEGINLPELFKYSEILDLRRLYSNDIHAMANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIQSSSSPLQQMTFETSFQFLKQATMMGSHDELKSPSACLVVGKVVKGGTGLFELKQPLR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
182	Ubiquitination	GSQGTHVKNVCESRS CCCCCCHHHHHHHHH	35.74	22790023
210	Phosphorylation	KQCPHCKTGRSVVRK HHCCCCCCCCCEECC	43.13	-
298	Phosphorylation	DDIGPESSFNPSMFF CCCCCHHHCCHHHEE	27.64	-
302	Phosphorylation	PESSFNPSMFFLDFI CHHHCCHHHEEEEEE	29.35	-
380	Phosphorylation	SSVAIDRSFLGLLPG CCEEECHHHHCCCCC	22.52	28066266
450	Phosphorylation	RVDYAARSVICPDMY CCCHHHHHCCCCCCE	15.88	28059163
471	Phosphorylation	GIPMVFATKLTYPQP CCCEEEEEECCCCCC	18.34	-
580	Ubiquitination	ARILPEEKVLRLHYA CCCCCHHHHHHHHHC	44.93	22790023
959	Phosphorylation	PRAGGFVTGRFLTGI CCCCCEECCCCCCCC	22.00	-
1077	Acetylation	QKVLGHIKAIKKWHH HHHHHHHHHHHHHHH	38.08	7616543
1080	Acetylation	LGHIKAIKKWHHKHS HHHHHHHHHHHHCCC	55.45	7616553
1113	Ubiquitination	AVKALNLKGSIQNGR HHHHHCCCCHHHCCC	49.92	22790023
1268	Phosphorylation	VASANIKTPMMSVPV HHCCCCCCCCCCCCC	16.78	25293948
1272	Phosphorylation	NIKTPMMSVPVFDTK CCCCCCCCCCCCCHH	19.60	25293948
1278	Phosphorylation	MSVPVFDTKKALKKV CCCCCCCHHHHHHHH	23.99	28576409
1287	Phosphorylation	KALKKVKSLKKRLTR HHHHHHHHHHHHHHH	48.70	28382018
1393	Phosphorylation	DTEDSGRSQREEERD CCCCCCHHHHHHHHH	36.80	-
1434	Phosphorylation	KQEEEVDYESEEEGE HHHHHCCCCCCCCCC	26.91	21149613
1436	Phosphorylation	EEEVDYESEEEGEEE HHHCCCCCCCCCCHH	44.04	25521595
1487	Phosphorylation	QKPPRRHSRPQGAEA CCCCCCCCCHHHHHH	43.79	28418008

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RPA1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RPA1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RPA1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TFE2_MOUSE	Tcf3	physical	20211142

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.