ROBO4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ROBO4_HUMAN
• UniProt AC: Q8WZ75
• Protein Name: Roundabout homolog 4
• Gene Name: ROBO4
• Organism: Homo sapiens (Human).
• Sequence Length: 1007
• Protein Description: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity)..
• Protein Sequence: MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MGSGGDSLLG -----CCCCCCCCCC	52.00	29759185
7	Phosphorylation	-MGSGGDSLLGGRGS -CCCCCCCCCCCCCH	28.86	29759185
30	Phosphorylation	MGGMAQDSPPQILVH HCCCCCCCCCEEEEC	26.80	25332170
131	Phosphorylation	VSRGARLSVAVLRED HHHCCEEEEEEHHCC	11.50	-
204	Phosphorylation	AEKSDEGTYMCVATN EEECCCCEEEEEEEC	13.16	-
212	Phosphorylation	YMCVATNSAGHRESR EEEEEECCCCCCCCC	31.13	-
232	O-linked_Glycosylation	IQEPQDYTEPVELLA CCCCCCCCCCHHHEE	41.94	OGP
246	N-linked_Glycosylation	AVRIQLENVTLLNPD EEEEEEEEEEEECCC	40.44	16335952
307	Phosphorylation	ELLAGWQSAELGGLH HHHHHHHHHCCCCCC	19.73	29759185
360	N-linked_Glycosylation	EVTLKPGNGTVFVSW EEEECCCCCEEEEEE	52.03	UniProtKB CARBOHYD
389	N-linked_Glycosylation	YQVWSLGNTSLPPAN EEEEEECCCCCCCCC	31.61	UniProtKB CARBOHYD
396	N-linked_Glycosylation	NTSLPPANWTVVGEQ CCCCCCCCEEEEECE	40.50	UniProtKB CARBOHYD
504	Phosphorylation	VHLGPGLYRYTSEDA CCCCCCCCEECCHHH	13.90	27642862
506	Phosphorylation	LGPGLYRYTSEDAIL CCCCCCEECCHHHHH	10.89	23403867
507	Phosphorylation	GPGLYRYTSEDAILK CCCCCEECCHHHHHH	19.35	23403867
508	Phosphorylation	PGLYRYTSEDAILKH CCCCEECCHHHHHHH	25.25	28857561
539	Phosphorylation	TSGSRDLSSSSSLSS CCCCCCCCCCCCHHH	32.25	18452278
541	Phosphorylation	GSRDLSSSSSLSSRL CCCCCCCCCCHHHHC	22.14	24719451
542	Phosphorylation	SRDLSSSSSLSSRLG CCCCCCCCCHHHHCC	36.93	28857561
543	Phosphorylation	RDLSSSSSLSSRLGA CCCCCCCCHHHHCCC	33.93	28857561
545	Phosphorylation	LSSSSSLSSRLGADA CCCCCCHHHHCCCCC	18.50	24719451
561	Phosphorylation	DPLDCRRSLLSWDSR CHHHHHHHHHHCCCC	17.82	23403867
564	Phosphorylation	DCRRSLLSWDSRSPG HHHHHHHHCCCCCCC	33.84	27251275
616	Phosphorylation	PQLAQLSSPCSSSDS HHHHHCCCCCCCCHH	38.22	29507054
631	Phosphorylation	LCSRRGLSSPRLSLA HHHHCCCCCCCCCCC	40.91	17081983
632	Phosphorylation	CSRRGLSSPRLSLAP HHHCCCCCCCCCCCC	21.05	24719451
636	Phosphorylation	GLSSPRLSLAPAEAW CCCCCCCCCCCHHHH	24.62	24501219
656	Phosphorylation	QELQHANSSPLLRGS HHHHHCCCCCCCCCC	33.83	23403867
657	Phosphorylation	ELQHANSSPLLRGSH HHHHCCCCCCCCCCC	21.26	23403867
663	Phosphorylation	SSPLLRGSHSLELRA CCCCCCCCCHHHEEE	11.94	27251275
665	Phosphorylation	PLLRGSHSLELRACE CCCCCCCHHHEEEEC	25.82	23403867
680	N-linked_Glycosylation	LGNRGSKNLSQSPGA CCCCCCCCCCCCCCC	46.77	UniProtKB CARBOHYD
682	Phosphorylation	NRGSKNLSQSPGAVP CCCCCCCCCCCCCHH	37.70	27251275
684	Phosphorylation	GSKNLSQSPGAVPQA CCCCCCCCCCCHHHH	23.17	23403867
704	Phosphorylation	ALGPKLLSSSNELVT HHCHHHHCCCCCCHH	42.00	28857561
705	Phosphorylation	LGPKLLSSSNELVTR HCHHHHCCCCCCHHH	36.83	28857561
706	Phosphorylation	GPKLLSSSNELVTRH CHHHHCCCCCCHHHC	30.83	28857561
778	Phosphorylation	SPASSRLSSSSLSSL CCCHHHCCHHHHHHC	27.36	22468782
781	Phosphorylation	SSRLSSSSLSSLGED HHHCCHHHHHHCCCC	33.93	22468782
783	Phosphorylation	RLSSSSLSSLGEDQD HCCHHHHHHCCCCCC	26.01	22468782
784	Phosphorylation	LSSSSLSSLGEDQDS CCHHHHHHCCCCCCC	45.76	22468782
791	Phosphorylation	SLGEDQDSVLTPEEV HCCCCCCCCCCHHHH	17.25	22468782
794	Phosphorylation	EDQDSVLTPEEVALC CCCCCCCCHHHHHHH	26.88	22468782
805	Phosphorylation	VALCLELSEGEETPR HHHHEECCCCCCCCC	33.89	24275569
816	Phosphorylation	ETPRNSVSPMPRAPS CCCCCCCCCCCCCCC	18.46	28857561
823	Phosphorylation	SPMPRAPSPPTTYGY CCCCCCCCCCCCCEE	42.39	23403867
826	Phosphorylation	PRAPSPPTTYGYISV CCCCCCCCCCEEEEC	35.71	23403867
827	Phosphorylation	RAPSPPTTYGYISVP CCCCCCCCCEEEECC	22.26	23403867
828	Phosphorylation	APSPPTTYGYISVPT CCCCCCCCEEEECCC	15.48	23403867
830	Phosphorylation	SPPTTYGYISVPTAS CCCCCCEEEECCCHH	4.61	23403867
891	Phosphorylation	NAASARASLVSSSDG CHHHHHHHEEECCCC	24.61	23403867
894	Phosphorylation	SARASLVSSSDGSFL HHHHHEEECCCCCHH	29.44	23403867
895	Phosphorylation	ARASLVSSSDGSFLA HHHHEEECCCCCHHH	25.60	23403867
899	Phosphorylation	LVSSSDGSFLADAHF EEECCCCCHHHHHHH	23.29	23403867
938	Phosphorylation	IDASSPPSPRDEIFL EECCCCCCCCHHHEE	35.63	24501219
1002	Phosphorylation	RMPKAGASPVDYS-- CCCCCCCCCCCCC--	25.25	23403867

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ROBO4_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ROBO4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ROBO4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SLIT2_HUMAN	SLIT2	physical	12941633
RXRA_HUMAN	RXRA	physical	14761960
PPARG_HUMAN	PPARG	physical	14761960
THB_HUMAN	THRB	physical	14761960

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction,hydrazide chemistry, and mass spectrometry.";
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E.,Moore R.J., Smith R.D.;
J. Proteome Res. 4:2070-2080(2005).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-246, AND MASSSPECTROMETRY.
PubMed: 16335952