RNF12_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RNF12_MOUSE
• UniProt AC: Q9WTV7
• Protein Name: E3 ubiquitin-protein ligase RLIM
• Gene Name: Rlim
• Organism: Mus musculus (Mouse).
• Sequence Length: 600
• Subcellular Localization: Nucleus .
• Protein Description: E3 ubiquitin-protein ligase that acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals..
• Protein Sequence: MENSDSNDKGSDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQSPDENRAGESSDDVTNSDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQTSENESEPSTRRLSVENMESSSQRQMENSASESASARPSRAERNSAEAVTEVPTTRAQRRARSRSPEHRRTRARAERSRSPLQPTSEIPRRAPTLEQSSENEPEGSSRTRHHVTLRQQISGPELLGRGLFAASGSRNPSQGTSSSDTGSNSESSGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQAPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPSASVSSRNVERVESRNGRGSSGGGNSSGSSSSSSPSPSSSGESSESSSEMFEGSSEGGSSGPSRRDGRHRAPVTFDESGSLPFLSLAQFFLLNEDDEDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLSSGNRESVV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MENSDSND -------CCCCCCCC	11.25	-
78	Phosphorylation	KEGPPPQSPDENRAG HCCCCCCCCCCCCCC	39.84	25521595
87	Phosphorylation	DENRAGESSDDVTNS CCCCCCCCCCCCCCC	37.84	26745281
88	Phosphorylation	ENRAGESSDDVTNSD CCCCCCCCCCCCCCH	33.26	26745281
148	Phosphorylation	NVNRNNGSQTSENES EECCCCCCCCCCCCC	32.20	18779572
150	Phosphorylation	NRNNGSQTSENESEP CCCCCCCCCCCCCCC	39.44	18779572
163	Phosphorylation	EPSTRRLSVENMESS CCCHHCCCHHHHHHH	26.10	27087446
169	Phosphorylation	LSVENMESSSQRQME CCHHHHHHHHHHHHH	25.23	28833060
170	Phosphorylation	SVENMESSSQRQMEN CHHHHHHHHHHHHHH	19.72	28833060
171	Phosphorylation	VENMESSSQRQMENS HHHHHHHHHHHHHHH	38.37	28833060
194	Phosphorylation	PSRAERNSAEAVTEV CCHHHHHCCHHHHCC	33.44	28833060
203	Phosphorylation	EAVTEVPTTRAQRRA HHHHCCCCHHHHHHH	33.80	25195567
204	Phosphorylation	AVTEVPTTRAQRRAR HHHCCCCHHHHHHHH	20.24	25195567
212	Phosphorylation	RAQRRARSRSPEHRR HHHHHHHHCCHHHHH	35.80	27717184
214	Phosphorylation	QRRARSRSPEHRRTR HHHHHHCCHHHHHHH	35.57	25266776
227	Phosphorylation	TRARAERSRSPLQPT HHHHHHHHCCCCCCC	28.72	27087446
229	Phosphorylation	ARAERSRSPLQPTSE HHHHHHCCCCCCCCC	31.07	27087446
234	Phosphorylation	SRSPLQPTSEIPRRA HCCCCCCCCCCCCCC	26.48	25619855
235	Phosphorylation	RSPLQPTSEIPRRAP CCCCCCCCCCCCCCC	39.96	25619855
243	Phosphorylation	EIPRRAPTLEQSSEN CCCCCCCCCCCCCCC	41.78	29899451
269	Phosphorylation	VTLRQQISGPELLGR EEEEHCCCCHHHHHC	43.91	25266776
284	Phosphorylation	GLFAASGSRNPSQGT CEEECCCCCCCCCCC	26.79	18779572
311	Phosphorylation	GSGQRPPTIVLDLQV CCCCCCCEEEEEEEE	26.80	22817900
340	Phosphorylation	ASRTRSRSQAPNNTV HHCCCCCCCCCCCEE	31.76	-
346	Phosphorylation	RSQAPNNTVTYESER CCCCCCCEEEEEECC	22.36	18779572
357	Methylation	ESERGGFRRTFSRSE EECCCCCEECCCHHH	39.75	30989223
526	Ubiquitination	DQPRGLTKEQIDNLA CCCCCCCHHHHHHHH	53.30	-
553	Phosphorylation	CSVCITEYTEGNKLR CCEEEEECCCCCCEE	11.05	-
558	Ubiquitination	TEYTEGNKLRKLPCS EECCCCCCEEECCCC	61.60	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RNF12_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RNF12_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RNF12_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ZFP42_MOUSE	Zfp42	physical	22596162

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227 AND SER-229, ANDMASS SPECTROMETRY.
PubMed: 17242355