RN216_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RN216_MOUSE
• UniProt AC: P58283
• Protein Name: E3 ubiquitin-protein ligase RNF216
• Gene Name: Rnf216
• Organism: Mus musculus (Mouse).
• Sequence Length: 853
• Subcellular Localization: Cytoplasm.
• Protein Description: Acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Promotes TNF and RIP mediated apoptosis..
• Protein Sequence: MAEGNNKEEVIHLNNFPCHRGKEWMAVREGPITISDSSDEEGIPMLVTPATEQQEDDLDDDVILTEDDSEDEYGGFLDLESGKKEGEAKPGPSSKQTADDIVNPRLEQKVIILGENGLLFPESEPLEVQNQSSEDSETELLSNPGEPAASVDDQLIGEEYWLDHPYFQAPNPQPQERTNQVVPQERHSESEMGPMFFRHDFPEPAFPRPEPQQEGIPGPASPQPAHPLGELEDQQLAIDEDPGPAFPLSGPQEANLANMWEQEAAEVDQDLIPLLVKETEARFPDVASGYVEEIIHLKNYYDLNVLCNFLLENPDYPKREDRLIIHPSSSLLASQDDAKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKKWQELSPETSGKRKKRKEMNQYSFIDFKFEQGNIKIEKRMFFLENKRRHCRYYDHQALLPAVKQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKSELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPRCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPAEKVQRVEALPRPVPQNLHPQMPPYAFVHPPFPLPPVRPVFNNFPINMGPVPAPYVPPLPNVRVNYDFGHMHVPLEHNLPMHFGPQPRHRF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Acetylation	NFPCHRGKEWMAVRE CCCCCCCCCEEEEEE	48.43	8275667
221	Phosphorylation	EGIPGPASPQPAHPL CCCCCCCCCCCCCCC	27.48	25338131
348	Phosphorylation	PKIDFFDYSKLTPLD CCCCCCCHHHCCCHH	11.53	21454597
350 (in isoform 2)	Ubiquitination	-	50.59	22790023
350	Ubiquitination	IDFFDYSKLTPLDQR CCCCCHHHCCCHHHH	50.59	22790023
352	Phosphorylation	FFDYSKLTPLDQRCF CCCHHHCCCHHHHHH	26.07	21454597
407	Phosphorylation	IKKWQELSPETSGKR HHHHHHCCCCCCCCH	21.67	21082442
407 (in isoform 2)	Ubiquitination	-	21.67	-
410	Phosphorylation	WQELSPETSGKRKKR HHHCCCCCCCCHHCH	46.28	26643407
411	Phosphorylation	QELSPETSGKRKKRK HHCCCCCCCCHHCHH	40.43	23984901
413	Acetylation	LSPETSGKRKKRKEM CCCCCCCCHHCHHHH	61.89	134135
464	Ubiquitination	QALLPAVKQEQEFYE HHHHHHHHHHHHHHH	50.66	22790023
464 (in isoform 2)	Ubiquitination	-	50.66	22790023
521 (in isoform 2)	Ubiquitination	-	5.24	-
595	Phosphorylation	DELVRCPSCSFPALL HHHCCCCCCCCCCHH	26.47	26160508
597	Phosphorylation	LVRCPSCSFPALLDS HCCCCCCCCCCHHCC	38.31	26160508
604	Phosphorylation	SFPALLDSDVKRFSC CCCCHHCCCCHHCCC	44.25	26160508
707	Phosphorylation	HFCQHPRSPGAPCQE CCCCCCCCCCCCCCC	31.94	26824392

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Rnf216	P58283	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RN216_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RN216_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ARC_MOUSE	Arc	physical	24945773
UB2L3_MOUSE	Ube2l3	physical	24945773

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.