ARC_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ARC_MOUSE
• UniProt AC: Q9WV31
• Protein Name: Activity-regulated cytoskeleton-associated protein {ECO:0000305}
• Gene Name: Arc {ECO:0000312|MGI:MGI:88067}
• Organism: Mus musculus (Mouse).
• Sequence Length: 396
• Subcellular Localization: Extracellular vesicle membrane ; Lipid-anchor . Cell junction, synapse, postsynaptic cell membrane ; Lipid-anchor . Cell junction, synapse . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Early endosome membrane . Cell projecti
• Protein Description: Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system (By similarity). ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation (By similarity). ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons (By similarity). Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. [PubMed: 29264923]
• Protein Sequence: MELDHMTTGGLHAYPAPRGGPAAKPNVILQIGKCRAEMLEHVRRTHRHLLTEVSKQVERELKGLHRSVGKLENNLDGYVPTGDSQRWKKSIKACLCRCQETIANLERWVKREMHVWREVFYRLERWADRLESMGGKYPVGSEPARHTVSVGVGGPEPYCQEADGYDYTVSPYAITPPPAAGELPEQESVEAQQYQSWGPGEDGQPSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELELPQKQGEPLDQFLWRKRDLYQTLYVDAEEEEIIQYVVGTLQPKLKRFLRHPLPKTLEQLIQRGMEVQDGLEQAAEPSGTPLPTEDETEALTPALTSESVASDRTQPE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
24	Ubiquitination	PRGGPAAKPNVILQI CCCCCCCCCCEEEEE	38.95	22790023
94	S-palmitoylation	WKKSIKACLCRCQET HHHHHHHHHHHHHHH	2.90	29264923
96	S-palmitoylation	KSIKACLCRCQETIA HHHHHHHHHHHHHHH	4.04	29264923
98	S-palmitoylation	IKACLCRCQETIANL HHHHHHHHHHHHHHH	4.12	29264923
252	Ubiquitination	HMNGPAKKWWEFKQG HCCCCCHHCHHHHCC	60.69	27667366
260	Phosphorylation	WWEFKQGSVKNWVEF CHHHHCCCCCCHHHH	27.68	31151856
269	Ubiquitination	KNWVEFKKEFLQYSE CCHHHHHHHHHHHCC	60.05	22790023
278	Phosphorylation	FLQYSEGTLSREAIQ HHHHCCCCCCHHHHH	19.94	31151856
280	Phosphorylation	QYSEGTLSREAIQRE HHCCCCCCHHHHHHH	28.10	25521595
293	Ubiquitination	RELELPQKQGEPLDQ HHHCCCHHCCCCHHH	59.33	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
260	S	Phosphorylation	Kinase	CAMK2	-	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Mdm2	P23804	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
260	S	Phosphorylation	Phosphorylation at Ser-260 by CaMK2 prevents homooligomerization into virion-like capsids by disrupting an interaction surface essential for high-order oligomerization.	31151856

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ARC_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DLG4_MOUSE	Dlg4	physical	28804447

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.