RN146_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RN146_RAT
• UniProt AC: Q5XIK5
• Protein Name: E3 ubiquitin-protein ligase RNF146
• Gene Name: Rnf146
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 352
• Subcellular Localization: Cytoplasm, cytosol. Nucleus. Translocates to the nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding (By similarity)..
• Protein Description: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity)..
• Protein Sequence: MAGCGEIDHSLNMLPTNKKASETCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYVWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDSNTVNLARESSADGADSGSAHTGASVQLPVPSSTRPLTSVDGQLTSPVTPSPDAGASLEDSFAHLQLSGDSIAERSHRGEGEEDHESPSSGRVPDTSTEETESDASSDIEDAPVVVAQHSLTQQRLLVSSANQTVAERSDRPVAGGGTMSVNVRSRRPDGQCTVTEV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
86	Phosphorylation	EDFLDKPTLLSPEEL HHHCCCCCCCCHHHH	46.18	25403869
89	Phosphorylation	LDKPTLLSPEELKAA CCCCCCCCHHHHHHH	33.12	30240740
272	Phosphorylation	EGEEDHESPSSGRVP CCCCCCCCCCCCCCC	27.02	28432305
274	Phosphorylation	EEDHESPSSGRVPDT CCCCCCCCCCCCCCC	55.49	28432305
275	Phosphorylation	EDHESPSSGRVPDTS CCCCCCCCCCCCCCC	33.72	28432305
281	Phosphorylation	SSGRVPDTSTEETES CCCCCCCCCCCCCCC	31.32	27097102
282	Phosphorylation	SGRVPDTSTEETESD CCCCCCCCCCCCCCC	41.00	27097102
283	Phosphorylation	GRVPDTSTEETESDA CCCCCCCCCCCCCCC	39.68	27097102
286	Phosphorylation	PDTSTEETESDASSD CCCCCCCCCCCCCCC	34.26	27097102
288	Phosphorylation	TSTEETESDASSDIE CCCCCCCCCCCCCCC	45.99	27097102
291	Phosphorylation	EETESDASSDIEDAP CCCCCCCCCCCCCCC	33.27	27097102
292	Phosphorylation	ETESDASSDIEDAPV CCCCCCCCCCCCCCE	44.31	27097102
305	Phosphorylation	PVVVAQHSLTQQRLL CEEEEECCCCCCHHH	22.49	27097102
307	Phosphorylation	VVAQHSLTQQRLLVS EEEECCCCCCHHHHH	26.18	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RN146_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RN146_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RN146_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PARP1_HUMAN	PARP1	physical	24842055

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.