RIFK_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RIFK_HUMAN
• UniProt AC: Q969G6
• Protein Name: Riboflavin kinase
• Gene Name: RFK
• Organism: Homo sapiens (Human).
• Sequence Length: 155
• Subcellular Localization: Cytoplasm.
• Protein Description: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase..
• Protein Sequence: MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
60	Acetylation	VGSGDVHKMVVSIGW ECCCCEEEEEEEECC	32.99	20167786
70	Phosphorylation	VSIGWNPYYKNTKKS EEECCCCCCCCCHHH	24.69	-
71	Phosphorylation	SIGWNPYYKNTKKSM EECCCCCCCCCHHHH	10.06	-
76	Acetylation	PYYKNTKKSMETHIM CCCCCCHHHHHHHHH	53.66	7493363
85	Phosphorylation	METHIMHTFKEDFYG HHHHHHHHHHHHHHH	21.01	-
127	Ubiquitination	QGDIEEAKKRLELPE HCCHHHHHHHCCCCH	41.53	-
137	Ubiquitination	LELPEHLKIKEDNFF CCCCHHHCCCCCCCE	54.35	-
139	Ubiquitination	LPEHLKIKEDNFFQV CCHHHCCCCCCCEEE	58.38	-
148	Ubiquitination	DNFFQVSKSKIMNGH CCCEEECHHHHCCCC	57.84	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RIFK_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RIFK_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RIFK_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GRHPR_HUMAN	GRHPR	physical	26344197
MEMO1_HUMAN	MEMO1	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• DB00140: Riboflavin