dbPTM

RHG29_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RHG29_HUMAN
UniProt AC	Q52LW3
Protein Name	Rho GTPase-activating protein 29
Gene Name	ARHGAP29
Organism	Homo sapiens (Human).
Sequence Length	1261
Subcellular Localization
Protein Description	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis..
Protein Sequence	MIAHKQKKTKKKRAWASGQLSTDITTSEMGLKSLSSNSIFDPDYIKELVNDIRKFSHMLLYLKEAIFSDCFKEVIHIRLEELLRVLKSIMNKHQNLNSVDLQNAAEMLTAKVKAVNFTEVNEENKNDLFQEVFSSIETLAFTFGNILTNFLMGDVGNDSLLRLPVSRETKSFENVSVESVDSSSEKGNFSPLELDNVLLKNTDSIELALSYAKTWSKYTKNIVSWVEKKLNLELESTRNMVKLAEATRTNIGIQEFMPLQSLFTNALLNDIESSHLLQQTIAALQANKFVQPLLGRKNEMEKQRKEIKELWKQEQNKMLEAENALKKAKLLCMQRQDEYEKAKSSMFRAEEEHLSSSGGLAKNLNKQLEKKRRLEEEALQKVEEANELYKVCVTNVEERRNDLENTKREILAQLRTLVFQCDLTLKAVTVNLFHMQHLQAASLADSLQSLCDSAKLYDPGQEYSEFVKATNSTEEEKVDGNVNKHLNSSQPSGFGPANSLEDVVRLPDSSNKIEEDRCSNSADITGPSFIRSWTFGMFSDSESTGGSSESRSLDSESISPGDFHRKLPRTPSSGTMSSADDLDEREPPSPSETGPNSLGTFKKTLMSKAALTHKFRKLRSPTKCRDCEGIVVFQGVECEECLLVCHRKCLENLVIICGHQKLPGKIHLFGAEFTQVAKKEPDGIPFILKICASEIENRALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQETKKNSLEDKKWPNMCIEINRILLKSKDLLRQLPASNFNSLHFLIVHLKRVVDHAEENKMNSKNLGVIFGPSLIRPRPTTAPITISSLAEYSNQARLVEFLITYSQKIFDGSLQPQDVMCSIGVVDQGCFPKPLLSPEERDIERSMKSLFFSSKEDIHTSESESKIFERATSFEESERKQNALGKCDACLSDKAQLLLDQEAESASQKIEDGKTPKPLSLKSDRSTNNVERHTPRTKIRPVSLPVDRLLLASPPNERNGRNMGNVNLDKFCKNPAFEGVNRKDAATTVCSKFNGFDQQTLQKIQDKQYEQNSLTAKTTMIMPSALQEKGVTTSLQISGDHSINATQPSKPYAEPVRSVREASERRSSDSYPLAPVRAPRTLQPQHWTTFYKPHAPIISIRGNEEKPASPSAAVPPGTDHDPHGLVVKSMPDPDKASACPGQATGQPKEDSEELGLPDVNPMCQRPRLKRMQQFEDLEGEIPQFV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Acetylation	HKQKKTKKKRAWASG CCCCCCHHHHHHHCC	53.36	7482737
12	Acetylation	KQKKTKKKRAWASGQ CCCCCHHHHHHHCCC	49.24	7482747
17	Phosphorylation	KKKRAWASGQLSTDI HHHHHHHCCCCCCCC	19.17	28102081
21	Phosphorylation	AWASGQLSTDITTSE HHHCCCCCCCCCCCH	19.02	23403867
22	Phosphorylation	WASGQLSTDITTSEM HHCCCCCCCCCCCHH	39.76	22777824
23	Ubiquitination	ASGQLSTDITTSEMG HCCCCCCCCCCCHHH	32.63	27667366
25	Phosphorylation	GQLSTDITTSEMGLK CCCCCCCCCCHHHHH	27.38	28102081
26	Phosphorylation	QLSTDITTSEMGLKS CCCCCCCCCHHHHHH	24.03	28102081
27	Phosphorylation	LSTDITTSEMGLKSL CCCCCCCCHHHHHHC	19.55	28102081
33	Phosphorylation	TSEMGLKSLSSNSIF CCHHHHHHCCCCCCC	38.23	27251275
35	Phosphorylation	EMGLKSLSSNSIFDP HHHHHHCCCCCCCCH	34.27	23090842
36	Phosphorylation	MGLKSLSSNSIFDPD HHHHHCCCCCCCCHH	39.63	27251275
38	Phosphorylation	LKSLSSNSIFDPDYI HHHCCCCCCCCHHHH	26.95	24245541
47	Ubiquitination	FDPDYIKELVNDIRK CCHHHHHHHHHHHHH	48.68	22817900
49	Ubiquitination	PDYIKELVNDIRKFS HHHHHHHHHHHHHHH	6.75	22817900
56	Phosphorylation	VNDIRKFSHMLLYLK HHHHHHHHHHHHHHH	15.83	23401153
87	Ubiquitination	EELLRVLKSIMNKHQ HHHHHHHHHHHHHCC	34.77	27667366
98	Phosphorylation	NKHQNLNSVDLQNAA HHCCCCCCCCHHHHH	21.85	30266825
106	Ubiquitination	VDLQNAAEMLTAKVK CCHHHHHHHHHHHHH	32.11	23000965
111	Ubiquitination	AAEMLTAKVKAVNFT HHHHHHHHHHCCCCC	38.75	22817900
113	Ubiquitination	EMLTAKVKAVNFTEV HHHHHHHHCCCCCCC	46.00	21906983
113 (in isoform 1)	Ubiquitination	-	46.00	21906983
113 (in isoform 2)	Ubiquitination	-	46.00	21906983
122	Ubiquitination	VNFTEVNEENKNDLF CCCCCCCHHHHCHHH	69.03	22817900
159	Phosphorylation	MGDVGNDSLLRLPVS HCCCCCCCCCCCCCC	33.05	24719451
169	Phosphorylation	RLPVSRETKSFENVS CCCCCCCCCCCCCCE	30.77	22777824
170	Ubiquitination	LPVSRETKSFENVSV CCCCCCCCCCCCCEE	48.68	23000965
170 (in isoform 1)	Ubiquitination	-	48.68	21906983
170 (in isoform 2)	Ubiquitination	-	48.68	21906983
171	Phosphorylation	PVSRETKSFENVSVE CCCCCCCCCCCCEEE	45.25	30266825
176	Phosphorylation	TKSFENVSVESVDSS CCCCCCCEEEEECCC	31.58	30266825
178	Ubiquitination	SFENVSVESVDSSSE CCCCCEEEEECCCCC	37.22	22817900
179	Phosphorylation	FENVSVESVDSSSEK CCCCEEEEECCCCCC	29.12	30266825
182	Phosphorylation	VSVESVDSSSEKGNF CEEEEECCCCCCCCC	33.34	30266825
183	Phosphorylation	SVESVDSSSEKGNFS EEEEECCCCCCCCCC	37.74	30266825
184	Phosphorylation	VESVDSSSEKGNFSP EEEECCCCCCCCCCC	47.48	23403867
186	Ubiquitination	SVDSSSEKGNFSPLE EECCCCCCCCCCCHH	62.19	22817900
186 (in isoform 1)	Ubiquitination	-	62.19	21906983
186 (in isoform 2)	Ubiquitination	-	62.19	21906983
190	Phosphorylation	SSEKGNFSPLELDNV CCCCCCCCCHHHHCE	32.35	25159151
214	Phosphorylation	LALSYAKTWSKYTKN HHHHHHHHHHHHHHH	27.14	-
220	Malonylation	KTWSKYTKNIVSWVE HHHHHHHHHHHHHHH	41.40	32601280
233	Ubiquitination	VEKKLNLELESTRNM HHHHHCCCCHHHHHH	49.79	30230243
242	Ubiquitination	ESTRNMVKLAEATRT HHHHHHHHHHHHHHC	31.07	21906983
242 (in isoform 1)	Ubiquitination	-	31.07	21906983
242 (in isoform 2)	Ubiquitination	-	31.07	21906983
247	Phosphorylation	MVKLAEATRTNIGIQ HHHHHHHHHCCCCCC	29.54	23532336
297	Ubiquitination	VQPLLGRKNEMEKQR HHHHHCCHHHHHHHH	56.30	30230243
305	Acetylation	NEMEKQRKEIKELWK HHHHHHHHHHHHHHH	62.39	19820965
355	Phosphorylation	RAEEEHLSSSGGLAK HHHHHHHHCCCHHHH	25.10	23927012
356	Phosphorylation	AEEEHLSSSGGLAKN HHHHHHHCCCHHHHH	39.09	30278072
357	Phosphorylation	EEEHLSSSGGLAKNL HHHHHHCCCHHHHHH	33.24	25159151
404	Ubiquitination	EERRNDLENTKREIL HHHHHHHHHHHHHHH	66.76	22817900
413	Ubiquitination	TKREILAQLRTLVFQ HHHHHHHHHHHHHHH	28.21	22817900
457	Phosphorylation	LCDSAKLYDPGQEYS HHHHCCCCCCCHHHH	21.27	27642862
459	Ubiquitination	DSAKLYDPGQEYSEF HHCCCCCCCHHHHHH	32.58	22817900
463	Phosphorylation	LYDPGQEYSEFVKAT CCCCCHHHHHHHHHC	12.95	27642862
468	Ubiquitination	QEYSEFVKATNSTEE HHHHHHHHHCCCCCC	55.61	21906983
468 (in isoform 1)	Ubiquitination	-	55.61	21906983
477	Ubiquitination	TNSTEEEKVDGNVNK CCCCCCCCCCCCHHH	49.24	22817900
477 (in isoform 1)	Ubiquitination	-	49.24	21906983
488	Phosphorylation	NVNKHLNSSQPSGFG CHHHCCCCCCCCCCC	36.39	23663014
489	Phosphorylation	VNKHLNSSQPSGFGP HHHCCCCCCCCCCCC	45.07	23663014
492	Phosphorylation	HLNSSQPSGFGPANS CCCCCCCCCCCCCCC	38.83	26329039
499	Phosphorylation	SGFGPANSLEDVVRL CCCCCCCCHHHHHCC	34.93	30266825
509	Phosphorylation	DVVRLPDSSNKIEED HHHCCCCCCCCCCCC	33.59	23403867
510	Phosphorylation	VVRLPDSSNKIEEDR HHCCCCCCCCCCCCC	49.90	23403867
512	Ubiquitination	RLPDSSNKIEEDRCS CCCCCCCCCCCCCCC	54.14	-
519	Phosphorylation	KIEEDRCSNSADITG CCCCCCCCCCCCCCC	34.41	19664994
521	Phosphorylation	EEDRCSNSADITGPS CCCCCCCCCCCCCCH	15.83	30266825
525	Phosphorylation	CSNSADITGPSFIRS CCCCCCCCCCHHHHH	43.52	23403867
528	Phosphorylation	SADITGPSFIRSWTF CCCCCCCHHHHHEEE	34.35	23403867
532	Phosphorylation	TGPSFIRSWTFGMFS CCCHHHHHEEEEECC	26.17	20068231
534	Phosphorylation	PSFIRSWTFGMFSDS CHHHHHEEEEECCCC	16.14	20068231
539	Phosphorylation	SWTFGMFSDSESTGG HEEEEECCCCCCCCC	31.19	25841592
541	Phosphorylation	TFGMFSDSESTGGSS EEEECCCCCCCCCCC	32.04	30576142
543	Phosphorylation	GMFSDSESTGGSSES EECCCCCCCCCCCCC	35.98	20068231
544	Phosphorylation	MFSDSESTGGSSESR ECCCCCCCCCCCCCC	41.32	30576142
547	Phosphorylation	DSESTGGSSESRSLD CCCCCCCCCCCCCCC	32.07	20068231
548	Phosphorylation	SESTGGSSESRSLDS CCCCCCCCCCCCCCC	42.75	20068231
550	Phosphorylation	STGGSSESRSLDSES CCCCCCCCCCCCCCC	29.17	20068231
552	Phosphorylation	GGSSESRSLDSESIS CCCCCCCCCCCCCCC	45.89	23927012
555	Phosphorylation	SESRSLDSESISPGD CCCCCCCCCCCCCCC	38.24	23927012
557	Phosphorylation	SRSLDSESISPGDFH CCCCCCCCCCCCCHH	32.11	30266825
559	Phosphorylation	SLDSESISPGDFHRK CCCCCCCCCCCHHHC	31.91	30266825
570	Phosphorylation	FHRKLPRTPSSGTMS HHHCCCCCCCCCCCC	26.11	28985074
572	Phosphorylation	RKLPRTPSSGTMSSA HCCCCCCCCCCCCCC	40.56	27050516
573	Phosphorylation	KLPRTPSSGTMSSAD CCCCCCCCCCCCCCH	39.21	23090842
575	Phosphorylation	PRTPSSGTMSSADDL CCCCCCCCCCCCHHC	18.81	28985074
577	Phosphorylation	TPSSGTMSSADDLDE CCCCCCCCCCHHCCC	23.39	23090842
578	Phosphorylation	PSSGTMSSADDLDER CCCCCCCCCHHCCCC	25.54	23090842
589	Phosphorylation	LDEREPPSPSETGPN CCCCCCCCCCCCCCC	52.34	30266825
591	Phosphorylation	EREPPSPSETGPNSL CCCCCCCCCCCCCCH	52.72	30266825
593	Phosphorylation	EPPSPSETGPNSLGT CCCCCCCCCCCCHHH	63.38	30266825
597	Phosphorylation	PSETGPNSLGTFKKT CCCCCCCCHHHHHHH	31.17	23090842
600	Phosphorylation	TGPNSLGTFKKTLMS CCCCCHHHHHHHHHH	37.26	23090842
620	Phosphorylation	HKFRKLRSPTKCRDC HHHHHCCCCCCCCCC	47.56	26329039
697	Ubiquitination	ICASEIENRALCLQG HHHHHHHHHHHHHHH	39.94	22817900
752	Ubiquitination	LYLRQLPEPFILFRL HHHHCCCCCCHHHHH	62.53	22817900
761	Ubiquitination	FILFRLYKEFIDLAK CHHHHHHHHHHHHHH	51.22	22817900
761 (in isoform 1)	Ubiquitination	-	51.22	21906983
803	Phosphorylation	INRILLKSKDLLRQL HHHHHHHCHHHHHCC	31.22	24825855
861	Phosphorylation	RPTTAPITISSLAEY CCCCCCEEHHHHHHH	17.47	27050516
868	Phosphorylation	TISSLAEYSNQARLV EHHHHHHHCCHHHHH	13.81	27642862
885	Phosphorylation	LITYSQKIFDGSLQP HHHHCHHHCCCCCCC	2.70	32645325
889	Phosphorylation	SQKIFDGSLQPQDVM CHHHCCCCCCCCCEE	26.19	28348404
898	Phosphorylation	QPQDVMCSIGVVDQG CCCCEEEEEEEECCC	12.32	28102081
913	Phosphorylation	CFPKPLLSPEERDIE CCCCCCCCHHHHHHH	37.64	19664994
922	Phosphorylation	EERDIERSMKSLFFS HHHHHHHHHHHHHHC	20.13	29514088
925	Phosphorylation	DIERSMKSLFFSSKE HHHHHHHHHHHCCHH	23.05	23403867
929	Phosphorylation	SMKSLFFSSKEDIHT HHHHHHHCCHHHHCC	32.92	23927012
930	Phosphorylation	MKSLFFSSKEDIHTS HHHHHHCCHHHHCCC	34.60	28355574
936	Phosphorylation	SSKEDIHTSESESKI CCHHHHCCCHHHHHH	34.23	23403867
937	Phosphorylation	SKEDIHTSESESKIF CHHHHCCCHHHHHHH	25.59	23403867
939	Phosphorylation	EDIHTSESESKIFER HHHCCCHHHHHHHHH	47.11	23403867
940	Phosphorylation	DIHTSESESKIFERA HHCCCHHHHHHHHHH	52.58	32645325
941	Phosphorylation	IHTSESESKIFERAT HCCCHHHHHHHHHHH	40.09	23403867
948	Phosphorylation	SKIFERATSFEESER HHHHHHHHCHHHHHH	39.92	30266825
949	Phosphorylation	KIFERATSFEESERK HHHHHHHCHHHHHHH	29.67	30266825
953	Phosphorylation	RATSFEESERKQNAL HHHCHHHHHHHHHHH	35.19	30266825
982	Ubiquitination	LDQEAESASQKIEDG HHHHHHHHHHHHHCC	13.90	30230243
991	Phosphorylation	QKIEDGKTPKPLSLK HHHHCCCCCCCCCCC	41.91	28555341
996	Phosphorylation	GKTPKPLSLKSDRST CCCCCCCCCCCCCCC	42.13	24501219
1010	Phosphorylation	TNNVERHTPRTKIRP CCCCCCCCCCCCCCC	22.23	28348404
1015	Ubiquitination	RHTPRTKIRPVSLPV CCCCCCCCCCCCCCC	6.26	29967540
1019	Phosphorylation	RTKIRPVSLPVDRLL CCCCCCCCCCCCCEE	29.64	30266825
1029	Phosphorylation	VDRLLLASPPNERNG CCCEEECCCCCCCCC	40.64	19664994
1037	Ubiquitination	PPNERNGRNMGNVNL CCCCCCCCCCCCCCH	33.25	30230243
1046	Ubiquitination	MGNVNLDKFCKNPAF CCCCCHHHHCCCCCC	56.69	30230243
1070	Ubiquitination	TTVCSKFNGFDQQTL HHHHHHHCCCCHHHH	54.69	29967540
1079	Ubiquitination	FDQQTLQKIQDKQYE CCHHHHHHHHHHHHH	45.51	29967540
1085	Phosphorylation	QKIQDKQYEQNSLTA HHHHHHHHHHCCCCH	25.81	28152594
1089	Phosphorylation	DKQYEQNSLTAKTTM HHHHHHCCCCHHEEE	27.13	28152594
1091	Phosphorylation	QYEQNSLTAKTTMIM HHHHCCCCHHEEEEC	26.40	28442448
1095	Phosphorylation	NSLTAKTTMIMPSAL CCCCHHEEEECCHHH	12.18	29759185
1100	Phosphorylation	KTTMIMPSALQEKGV HEEEECCHHHHHCCC	25.00	29759185
1108	Phosphorylation	ALQEKGVTTSLQISG HHHHCCCCEEEEECC	21.24	28348404
1109	Phosphorylation	LQEKGVTTSLQISGD HHHCCCCEEEEECCC	25.65	28348404
1110	Phosphorylation	QEKGVTTSLQISGDH HHCCCCEEEEECCCC	14.65	28348404
1114	Phosphorylation	VTTSLQISGDHSINA CCEEEEECCCCCCCC	25.80	28442448
1118	Phosphorylation	LQISGDHSINATQPS EEECCCCCCCCCCCC	23.44	28442448
1122	Phosphorylation	GDHSINATQPSKPYA CCCCCCCCCCCCCCC	36.20	28442448
1125	Phosphorylation	SINATQPSKPYAEPV CCCCCCCCCCCCCCC	36.12	28442448
1128	Phosphorylation	ATQPSKPYAEPVRSV CCCCCCCCCCCCCCH	27.60	28442448
1139	Phosphorylation	VRSVREASERRSSDS CCCHHHHHHHCCCCC	27.21	28555341
1143	Phosphorylation	REASERRSSDSYPLA HHHHHHCCCCCCCCC	44.72	30266825
1144	Phosphorylation	EASERRSSDSYPLAP HHHHHCCCCCCCCCC	28.85	30266825
1146	Phosphorylation	SERRSSDSYPLAPVR HHHCCCCCCCCCCCC	30.51	30266825
1147	Phosphorylation	ERRSSDSYPLAPVRA HHCCCCCCCCCCCCC	13.78	30266825
1185	Phosphorylation	GNEEKPASPSAAVPP CCCCCCCCCCCCCCC	28.10	25159151
1187	Phosphorylation	EEKPASPSAAVPPGT CCCCCCCCCCCCCCC	26.77	25159151
1194	Phosphorylation	SAAVPPGTDHDPHGL CCCCCCCCCCCCCCC	35.61	23403867
1220	Phosphorylation	SACPGQATGQPKEDS CCCCCCCCCCCCCCH	28.58	27251275
1227	Phosphorylation	TGQPKEDSEELGLPD CCCCCCCHHHHCCCC	33.78	28348404

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of RHG29_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RHG29_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RHG29_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
KDM1A_HUMAN	KDM1A	physical	23455924
HERC1_HUMAN	HERC1	physical	26186194

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RHG29_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-930, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; SER-176; SER-179;SER-190; SER-949; SER-1029 AND SER-1146, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-913 AND SER-1019, ANDMASS SPECTROMETRY.	17081983 [Abstract]