RAD54_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RAD54_HUMAN
• UniProt AC: Q92698
• Protein Name: DNA repair and recombination protein RAD54-like
• Gene Name: RAD54L
• Organism: Homo sapiens (Human).
• Sequence Length: 747
• Subcellular Localization: Nucleus .
• Protein Description: Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells..
• Protein Sequence: MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQHEAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLPVHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRLHVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTAHEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQTELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKALEPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFNSPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEKIFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCTSDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MRRSLAPSQLA ----CCCCCCHHHHH	20.65	25159151
12	Acetylation	LAPSQLAKRKPEGRS CCHHHHHHCCCCCCC	70.33	25953088
19	Phosphorylation	KRKPEGRSCDDEDWQ HCCCCCCCCCCCCCC	32.52	28450419
31	Phosphorylation	DWQPGLVTPRKRKSS CCCCCCCCCCCCCCC	23.55	21815630
37	Phosphorylation	VTPRKRKSSSETQIQ CCCCCCCCCCHHHHH	43.03	28450419
38	Phosphorylation	TPRKRKSSSETQIQE CCCCCCCCCHHHHHH	34.92	25159151
39	Phosphorylation	PRKRKSSSETQIQEC CCCCCCCCHHHHHHH	52.32	28450419
41	Phosphorylation	KRKSSSETQIQECFL CCCCCCHHHHHHHHH	32.68	28450419
49	Phosphorylation	QIQECFLSPFRKPLS HHHHHHHHCCCCCHH	11.18	17081983
53	Ubiquitination	CFLSPFRKPLSQLTN HHHHCCCCCHHHHHC	51.17	-
76	Phosphorylation	QHEAFIRSILSKPFK HHHHHHHHHHCCCCC	24.06	24719451
80	Ubiquitination	FIRSILSKPFKVPIP HHHHHHCCCCCCCCC	51.53	-
83	Ubiquitination	SILSKPFKVPIPNYQ HHHCCCCCCCCCCCC	56.48	-
115	Ubiquitination	ALHDPLEKDALVLYE HHCCCCCCCCEEEEC	56.86	-
121	Phosphorylation	EKDALVLYEPPPLSA CCCCEEEECCCCCCH	20.93	27642862
133	Ubiquitination	LSAHDQLKLDKEKLP CCHHHHHCCCHHHCC	49.35	-
138	Ubiquitination	QLKLDKEKLPVHVVV HHCCCHHHCCCEEEE	64.92	-
176	Phosphorylation	TSRRIPGSHGCIMAD HCCCCCCCCCCEEEC	16.02	29507054
208	Ubiquitination	LRQSPECKPEIDKAV HHHCCCCCCCCCEEE	43.98	-
224	Ubiquitination	VSPSSLVKNWYNEVG ECHHHHHHHHHHHCH	47.24	-
232	Ubiquitination	NWYNEVGKWLGGRIQ HHHHHCHHHHCCCCE	44.41	-
247	Phosphorylation	PLAIDGGSKDEIDQK EEEECCCCHHHHHHH	42.32	-
248	Ubiquitination	LAIDGGSKDEIDQKL EEECCCCHHHHHHHH	63.76	-
254	Ubiquitination	SKDEIDQKLEGFMNQ CHHHHHHHHHHHHHH	44.77	-
302	Ubiquitination	CDEGHRLKNSENQTY CCCCCCCCCCCCHHH	59.38	2190698
308	Phosphorylation	LKNSENQTYQALDSL CCCCCCHHHHHHHHC	29.23	28796482
309	Phosphorylation	KNSENQTYQALDSLN CCCCCHHHHHHHHCC	5.09	28796482
314	Phosphorylation	QTYQALDSLNTSRRV HHHHHHHHCCCCCCE	25.11	21712546
355	Ubiquitination	GTAHEFKKHFELPIL CCHHHHHHHCCCCCC	59.87	-
363	Ubiquitination	HFELPILKGRDAAAS HCCCCCCCCHHHHHH	53.25	-
385	Phosphorylation	EERLRELTSIVNRCL HHHHHHHHHHHHHHH	16.20	22468782
386	Phosphorylation	ERLRELTSIVNRCLI HHHHHHHHHHHHHHH	36.84	22468782
396	Phosphorylation	NRCLIRRTSDILSKY HHHHHHCHHHHHHHH	22.39	24719451
402	Ubiquitination	RTSDILSKYLPVKIE CHHHHHHHHCCCEEE	47.57	-
407	Ubiquitination	LSKYLPVKIEQVVCC HHHHCCCEEEEEEEC	38.29	-
425	Ubiquitination	PLQTELYKRFLRQAK HHCHHHHHHHHHHCC	49.66	-
432	Ubiquitination	KRFLRQAKPAEELLE HHHHHHCCCHHHHHC	36.10	-
441	Ubiquitination	AEELLEGKMSVSSLS HHHHHCCCCCHHHHH	20.73	-
454	Ubiquitination	LSSITSLKKLCNHPA HHHHHHHHHHHCCCH	43.39	-
455	Ubiquitination	SSITSLKKLCNHPAL HHHHHHHHHHCCCHH	64.66	-
503	Phosphorylation	GKMLVLDYILAVTRS CCHHHHHHHHHHHCC	8.15	22817900
515	Acetylation	TRSRSSDKVVLVSNY HCCCCCCCEEEEECC	35.87	32457312
522	Phosphorylation	KVVLVSNYTQTLDLF CEEEEECCHHHHHHH	7.85	30631047
548	Phosphorylation	VRLDGTMSIKKRAKV EEECCCEEHHHHHHH	30.97	24719451
550	Ubiquitination	LDGTMSIKKRAKVVE ECCCEEHHHHHHHHH	28.97	-
572	Phosphorylation	PDFVFMLSSKAGGCG CCEEEEECCCCCCCC	20.29	27264870
623	Phosphorylation	CYIYRLLSAGTIEEK EEEEEHHHCCCHHHH	29.28	28555341
630	Ubiquitination	SAGTIEEKIFQRQSH HCCCHHHHHHHHHHH	35.71	-
639	Ubiquitination	FQRQSHKKALSSCVV HHHHHHHHHHHHCCC	49.49	-
662	Ubiquitination	HFSLGELKELFILDE HCCHHHHHHEEEEEC	48.33	-
671	Phosphorylation	LFILDEASLSDTHDR EEEEECCCCCCCCCH	26.76	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
31	T	Phosphorylation	Kinase	CDK2	P24941	PSP
49	S	Phosphorylation	Kinase	CDK2	P24941	PSP
572	S	Phosphorylation	Kinase	NEK1	Q96PY6	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
572	S	Phosphorylation	Phosphorylation at Ser-572 by NEK1 specifically in G2 phase allows efficient removal of RAD51 filaments from DNA.	27264870

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RAD54_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NTAQ1_HUMAN	WDYHV1	physical	25416956

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.