RAC1_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RAC1_RAT
• UniProt AC: Q6RUV5
• Protein Name: Ras-related C3 botulinum toxin substrate 1
• Gene Name: Rac1
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 192
• Subcellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Found in the ruffled border (a late endosomal-like compa
• Protein Description: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3..
• Protein Sequence: MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
64	Phosphorylation	DTAGQEDYDRLRPLS CCCCCCCHHHCCCCC	11.38	22276854
116	Ubiquitination	PIILVGTKLDLRDDK CEEEEEECCCCCCCH	33.79	-
123	Acetylation	KLDLRDDKDTIEKLK CCCCCCCHHHHHHHH	62.15	72600163
123	Ubiquitination	KLDLRDDKDTIEKLK CCCCCCCHHHHHHHH	62.15	-
128	Ubiquitination	DDKDTIEKLKEKKLT CCHHHHHHHHHCCCC	62.09	-
133	Ubiquitination	IEKLKEKKLTPITYP HHHHHHCCCCCCCCH	59.70	-
147	Acetylation	PQGLAMAKEIGAVKY HHHHHHHHHHCCEEE	36.46	22902405
147	Ubiquitination	PQGLAMAKEIGAVKY HHHHHHHHHHCCEEE	36.46	-
153	Acetylation	AKEIGAVKYLECSAL HHHHCCEEEEECHHH	43.51	22902405
166	Acetylation	ALTQRGLKTVFDEAI HHHHHCHHHHHHHHH	45.53	24217241
166	Ubiquitination	ALTQRGLKTVFDEAI HHHHHCHHHHHHHHH	45.53	-
167	Phosphorylation	LTQRGLKTVFDEAIR HHHHCHHHHHHHHHH	31.29	25403869
183	Ubiquitination	VLCPPPVKKRKRKCL HCCCCCCCCCCCCEE	53.27	-
184	Ubiquitination	LCPPPVKKRKRKCLL CCCCCCCCCCCCEEC	64.48	-
189	Methylation	VKKRKRKCLLL---- CCCCCCCEECC----	3.62	-
189	Geranylgeranylation	VKKRKRKCLLL---- CCCCCCCEECC----	3.62	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RAC1_RAT !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RAC1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RAC1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TBA1A_RAT	Tuba1a	physical	8631991
TBB2B_RAT	Tubb2b	physical	8631991
MK01_RAT	Mapk1	physical	19356729
KPCD_RAT	Prkcd	physical	19356729

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.