PTN5_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PTN5_MOUSE
• UniProt AC: P54830
• Protein Name: Tyrosine-protein phosphatase non-receptor type 5
• Gene Name: Ptpn5
• Organism: Mus musculus (Mouse).
• Sequence Length: 541
• Subcellular Localization: Isoform STEP61: Endoplasmic reticulum membrane; Multi-pass membrane protein.; Isoform STEP46: Cytoplasm.
• Protein Description: May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors..
• Protein Sequence: MCCSERLLGLPQPVEMEAPDEAEGLPSKQKEMPPPPPPSPPSEPAQKLPPQGAGSHSLTVRSSLCLFAASQFLLACGVLWLSGHGHSWLQNTTDLISSSLTVLNHLGPVAWLGSGTWGIPSLLLVSLTVSLVIVTTLVWHLLKAPPEPPAPLPPEDRRQSVSRQPSFTYSEWMEEKVEDDFLDLDAVPETPVFDCVMDIKPETDPASLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLVSPREESAHEYLLSASRVLRAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVRLTSPDPEDPLSSYINANYIRGYSGEEKVYIATQGPIVSTVADFWRMVWQERTPIIVMITNIEEMNEKCTEYWPEEQVVHDGVEITVQKVIHTEDYRLRLISLRRGTEERSLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCSPIIVHCSAGIGRTGCFIATSICCQQLRREGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHAMSLYEKQLSLQSSE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
160	Phosphorylation	PPEDRRQSVSRQPSF CHHHHCCCCCCCCCC	21.90	29899451
162	Phosphorylation	EDRRQSVSRQPSFTY HHHCCCCCCCCCCCH	30.37	29899451
166	Phosphorylation	QSVSRQPSFTYSEWM CCCCCCCCCCHHHHH	23.14	25521595
168	Phosphorylation	VSRQPSFTYSEWMEE CCCCCCCCHHHHHHH	30.40	29899451
169	Phosphorylation	SRQPSFTYSEWMEEK CCCCCCCHHHHHHHH	11.55	29899451
170	Phosphorylation	RQPSFTYSEWMEEKV CCCCCCHHHHHHHHH	23.02	29899451
190	Phosphorylation	DLDAVPETPVFDCVM CCCCCCCCCCCEEEE	20.77	29899451
221	Phosphorylation	GLQERRGSNVSLTLD CCCCCCCCCCEEEEE	32.04	7832766
224	Phosphorylation	ERRGSNVSLTLDMCT CCCCCCCEEEEEEEC	21.64	29899451
231	Phosphorylation	SLTLDMCTPGCNEEG EEEEEEECCCCCCCC	18.97	-
244	Phosphorylation	EGFGYLVSPREESAH CCCCEEECCCCCHHH	18.68	-
318	Phosphorylation	PHSRVRLTSPDPEDP CCCCCCCCCCCCCCC	27.87	29899451
319	Phosphorylation	HSRVRLTSPDPEDPL CCCCCCCCCCCCCCH	31.71	29899451
338	Phosphorylation	NANYIRGYSGEEKVY CHHEECCCCCCCEEE	12.03	26160508
339	Phosphorylation	ANYIRGYSGEEKVYI HHEECCCCCCCEEEE	42.42	26160508
345	Phosphorylation	YSGEEKVYIATQGPI CCCCCEEEEEECCCC	8.91	26160508
348	Phosphorylation	EEKVYIATQGPIVST CCEEEEEECCCCCHH	25.21	26160508
422	O-linked_Glycosylation	LISLRRGTEERSLKH EEEECCCCCCCCCCC	32.63	30059200
540	Phosphorylation	KQLSLQSSE------ HHHHHHCCC------	34.07	29899451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
221	S	Phosphorylation	Kinase	PKACA	P17612	PSP
221	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
221	S	Phosphorylation	Kinase	PKA	-	Uniprot
231	T	Phosphorylation	Kinase	MAPK	-	Uniprot
244	S	Phosphorylation	Kinase	MAPK	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
221	S	Phosphorylation	Phosphorylation at Ser-221 by PKA deactivates PTPN5.	-
231	T	Phosphorylation	Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase (By similarity).	-
231	T	ubiquitylation	Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase (By similarity).	-
244	S	Phosphorylation	Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase (By similarity).	-
244	S	ubiquitylation	Phosphorylation at Thr-231 and Ser-244 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PTN5_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DLG4_MOUSE	Dlg4	physical	27457929
NMDE1_MOUSE	Grin2a	physical	27457929
NMDE2_MOUSE	Grin2b	physical	27457929
FYN_MOUSE	Fyn	physical	27457929

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.